Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(R473A)

Retroviral expression vector of human KLF4, mutant R473A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19012_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(R473A) 5') Sequence file: RDB19012_B1Eca.seq
>D07716A9_B1Ec_1_MMLV-pol_F_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG NATCGNAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC    61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA   121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG   181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT   241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA   301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC   361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG   421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG   481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG   541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC   601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG   661 GCGTGGCGCC GGGCGGCACG GGCGGAGGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC   721 CGACGGCTCC CTTCAACCTG GCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG   781 CCGANCTCCT GCGGCCAGAA TTGGACCCGG NGTACATTCC GCCGCANCAG CCGCAGCCGC   841 CAGGTGNCGG GCTGATGGGC AAGTTCGTGC TGAAGGCGTC GCTGANCGCC CNNNGCAGCN   901 NNTACNGCAG CCCGNCNGNC ATCAGCGTCA GCAAANGNAG CCNNGANNNN NNCCNNNNNG 961 NGGNNGNNNN NNNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4(R473A) 3') Sequence file: RDB19012_B1Ecb.seq
>D07716A9_B1Ec_1_pMXs-3LTRup_R2_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTN NTCGTCGNNN NCTGTGCTGG CGGCCGCTTA AAAATGCCTC    61 TTCATGTGTA AGGCGAGGTG GTCCGACCTG GAAAATGCTC GGTCGCATTT TTGGCACTGG   121 AACGGGCGGT GCCCCGTGTG TTTACGGTAG TGCCTGGTCA GTTCATCTGA GGCGGCGAAT   181 TTCCATCCAC AGCCGTCCCA GTCACAGTGG TAAGGTTTCT CACCTGTGTG GGTTCGCAGG   241 TGTGCCTTGA GATGGGAACT CTTTGTGTAG GTTTTGCCGC AGCCCGCGTA ATCACAAGTG   301 TGGGTGGCGG TCCTTTTCCG GGGCCACGAT CGTCTTCCCC TCTTTGGCTT GGGCTCCTCT   361 GGCATGCAGG AACCGGGTGG CATGAGCTCT TGGTAATGGA GCGGCGGGAC TTGCGGCTGC   421 ATCTGATCGG GCAGGAAGGA TGGGTAATTG GGCCCCGGGT GGGGATGGAA GCCGGGAGGA   481 AGCGGCAGGG CAGGGTGACA GTCCCTGCTG CTCAGCACTT CCTCAAGACC CAGGGTCGGG   541 GTAGTCCTGC TGGGGAGCTG CCGCCCCAGG GGGAAGTCGT GTGCAGCCGG CCGGTGGCCA   601 TTGCTGAGAG GGGGTCCAGC GCCCAAGTGG GTGCACGAAG AGACCGCCTC CTGCTTGATC   661 TTGGGGCACG TGCGCGGCGG CCCGCCGTTG TAGGGCGCCA CCACCACCGG GTGGCTGCCG   721 TCAGGGCTGC CTTTGCTGAC GCTGATGANC GACGGGCTGC CGTACTCGCT GCCANGGGCG   781 CTCAGCGACG CCTTCANCAC GAACTTGCCC ATCAGCCCGC CACCTGGCGG CTGCGGCTGC   841 TGCGNCGGAN NGTACACCGG GTCCNATNCT GGNCGCANGA NCTCGGCCNC NANNNNGCCC   901 NANGGGCTCN CGTCNTNNAT GTNCNCNNGG TTNAANGNNN NNGTCGGANG GGGNNCGGAC 961 TNCCTNNNNN ANNNGANNNC NNNNNNNNNG NNNNNNNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19012_B1Ecc.seq
>D07716A9_B1Ec_1_gag-N_R_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTTTTNNGAC AAATACAGAA ACACAGTCAG    61 ACAGAGACAA CACAGAACGA TGCTGCAGCA GACAAGACGC GCGGCGCGGC TTCGGTCCCA   121 AACCGAAAGC AAAAATTCAG ACGGAGGCGG GAACTGTTTT AGGTTCTCGT CTCCTACCAG   181 AACCACATAT CCCTCCTATT AGGGGGGTGC ACCAAAGAGT CCAAAACGAT CGGGATTTTT   241 GGACTCAGGT CGGGCCACAA AAACGGCCCC CGAAGTCCCT GGGACGTCTC CCAGGGTTGC   301 GGCCGGGTGT TCCGAACTCG TCAGTTCCAC CACGGGTCCG CCAGATACAG AGCTAGTTAG   361 CTAACTAGTA CCGACGCAGG CGCATAAAAT CAGTCATAGA CACTAGACAA TCGGACAGAC   421 ACAGATAAGT TGCTGGCCAG CTTACCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG   481 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCG CTGACGGGTA GTCAATCACT   541 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT   601 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC GCCGAGTGAG   661 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG ANCAGAAGCG CGCGAACAGA AGCGAGAAGC   721 GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC ANGTCCTTGG GGCACCCTGG   781 AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAN CGCATCTGGG GANCATCTGT   841 TCTTGGNCCT GANCCNGGGC ANGAACTGCT TACCACAGAT ATCCTGTTNN GCCCNTATTC   901 AGCNNNTNCA TCNGNTCTNN GNCCNNANNC NGGGGNNNNN NNNGNNNNNN NNANNNATNN 961 NGTTTNGNCN NNANNNNGCN GNTNNNNNNN NNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19012_B1Ecd.seq
>D07716A9_B1Ec_1_lacIpro-B_F_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CNNNNNNTNN GTGANCGANG AANCGGAAGA NCGCCCAATA    61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG   121 TAAATACTTA AGAAAAAAAA CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC   181 GCTGACGGGT AGTCAATCAC TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC   241 GGATGCAACT GCAAGAGGGT TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG   301 AGGACTGGCG CCCCGAGTGA GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC   361 GCGCGAACAG AAGCGAGAAG CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT   421 CAGGTCCTTG GGGCACCCTG GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA   481 CCGCATCTGG GGACCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA   541 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA   601 ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT   661 TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTTCCAT GCCTTGCAAA ATGGCGTTAC   721 TTAAGCTAGC TTGCCNAACC TACAGGTGGG GTCTTTCATT CCCCCCTTTT TCTGGAGACT   781 NAANTAAAAT CTTTTATTTT ATCGTCNANC ACTGTGCTGG CGGCCGCTTA AAANTGCCTC   841 TTCATGTGTA ANGNGAGGTG GNCCGACCTG GAAAATGNTC GGTCGCNTTT TTGGCACNNG   901 AACGGGNNGN GCCCCGNGTG TTTACNGNAG TGNCTGGNCA NTTCATCTGN NNNGCGAANT 961 TTCCNTCCNC AGCCNNCCNN NNCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

GNP_filter3_RDBDEP_html_230702.pl