Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(D475A)

Retroviral expression vector of human KLF4, mutant D475A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19013_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(D475A) 5') Sequence file: RDB19013_B1Eca.seq
>D07716B1_B1Ec_2_MMLV-pol_F_H04_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCGCAN CTTGGNNACN CGCCGCCCAC GTGNAGGCTG    61 CCGACCCCGG GGGTGGACCA TCCTCTAGAC TGCCGGATCT AGCTAGTTAA TTAAGGATCC   121 CAGTGTGGTG GTACGGGAAT TCATGGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA   181 TGACATCGAC TACAAGGATG ACGATGACAA GGCTGTCAGC GACGCGCTGC TCCCATCTTT   241 CTCCACGTTC GCGTCTGGCC CGGCGGGAAG GGAGAAGACA CTGCGTCAAG CAGGTGCCCC   301 GAATAACCGC TGGCGGGAGG AGCTCTCCCA CATGAAGCGA CTTCCCCCAG TGCTTCCCGG   361 CCGCCCCTAT GACCTGGCGG CGGCGACCGT GGCCACAGAC CTGGAGAGCG GCGGAGCCGG   421 TGCGGCTTGC GGCGGTAGCA ACCTGGCGCC CCTACCTCGG AGAGAGACCG AGGAGTTCAA   481 CGATCTCCTG GACCTGGACT TTATTCTCTC CAATTCGCTG ACCCATCCTC CGGAGTCAGT   541 GGCCGCCACC GTGTCCTCGT CAGCGTCAGC CTCCTCTTCG TCGTCGCCGT CGAGCAGCGG   601 CCCTGCCAGC GCGCCCTCCA CCTGCAGCTT CACCTATCCG ATCCGGGCCG GGAACGACCC   661 GGGCGTGGCG CCGGGCGGCA CGGGCGGANG CCTCCTCTAT GGCAGGGAGT CCGCTCCCCC   721 TCCGACGGCT CCCTTCAACC TGGCGGACAT CAACGACGTG AGCCCCTCGG GCGGCTTCGT   781 GGCCGANCTC CTGCGGCCAG AATTGGACCC NGTGTACATT CCGCCGNANC ANCCGCANCC   841 GCCAGGTGGC GGGCTGATGG GCAAGTTCGT GCTGNANGNG TCGCTNANCG CCCCTGGCAN   901 CGANTANGGN ANCCCGTCNG TCATCNNCGN NNNCNNANGN NANNCNNGNN NGGNAGCCAN 961 CCNNNNGGNN NNGGNGCCNN ANNNCNGNNG GNNNNNNNNN N //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4(D475A) 3') Sequence file: RDB19013_B1Ecb.seq
>D07716B1_B1Ec_2_pMXs-3LTRup_R2_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNCGTCGNN NNCTGTGCTG GCGGCCGCTT AAAAATGCCT    61 CTTCATGTGT AAGGCGAGGT GGTCCGACCT GGAAAATGCT CGGTCGCATT TTTGGCACTG   121 GAACGGGCGG TGCCCCGTGT GTTTACGGTA GTGCCTGGTC AGTTCAGCTG AGCGGGCGAA   181 TTTCCATCCA CAGCCGTCCC AGTCACAGTG GTAAGGTTTC TCACCTGTGT GGGTTCGCAG   241 GTGTGCCTTG AGATGGGAAC TCTTTGTGTA GGTTTTGCCG CAGCCCGCGT AATCACAAGT   301 GTGGGTGGCG GTCCTTTTCC GGGGCCACGA TCGTCTTCCC CTCTTTGGCT TGGGCTCCTC   361 TGGCATGCAG GAACCGGGTG GCATGAGCTC TTGGTAATGG AGCGGCGGGA CTTGCGGCTG   421 CATCTGATCG GGCAGGAAGG ATGGGTAATT GGGCCCCGGG TGGGGATGGA AGCCGGGAGG   481 AAGCGGCAGG GCAGGGTGAC AGTCCCTGCT GCTCAGCACT TCCTCAAGAC CCAGGGTCGG   541 GGTAGTCCTG CTGGGGAGCT GCCGCCCCAG GGGGAAGTCG TGTGCAGCCG GCCGGTGGCC   601 ATTGCTGAGA GGGGGTCCAG CGCCCAAGTG GGTGCACGAA GAGACCGCCT CCTGCTTGAT   661 CTTGGGGCAC GTGCGCGGCG GCCCGCCGTT GTAGGGCGCC ACCACCACCG GGTGGCTGCC   721 GTCAGGGCTG CCTTTGCTGA CGCTGATGAN CGACGGGCTG CCGTACTCGC TGCCANGGGC   781 GCTCAGCGAC GCCTTCAGCA CGAACTTGCC CATCAGCCCG CCACCTGGCG GCTGCGGCTG   841 CTGCGGCGGA NTGTACACCG GGGNCCNATT CTGGCCGCNN GANCTCNGNC NNNAAGCCNC   901 CCNANGGGCT CACGTCNNTN ATGNCCNCCA GGTTGAANGN NNCCNNNNNA NGGGGANCGN 961 NNTCCCTGNC NTANANNANN NNNNNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19013_B1Ecc.seq
>D07716B1_B1Ec_2_gag-N_R_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTTTNNGANN NNNNACNGAA ACACAGTCAG    61 ACAGAGACAA CACAGAACGA TGCTGCAGCA GACAAGACGC GCGGCGCGGC TTCGGTCCCA   121 AACCGAAAGC AAAAATTCAG ACGGAGGCGG GAACTGTTTT AGGTTCTCGT CTCCTACCAG   181 AACCACATAT CCCTCCTATT AGGGGGGTGC ACCAAAGAGT CCAAAACGAT CGGGATTTTT   241 GGACTCAGGT CGGGCCACAA AAACGGCCCC CGAAGTCCCT GGGACGTCTC CCAGGGTTGC   301 GGCCGGGTGT TCCGAACTCG TCAGTTCCAC CACGGGTCCG CCAGATACAG AGCTAGTTAG   361 CTAACTAGTA CCGACGCAGG CGCATAAAAT CAGTCATAGA CACTAGACAA TCGGACAGAC   421 ACAGATAAGT TGCTGGCCAG CTTACCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG   481 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCG CTGACGGGTA GTCAATCACT   541 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT   601 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGANTGGCGC GCCGAGTGAG   661 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC   721 GAACTGATTG GTTAGTTCAA ATAANGCACA GGGTCATTTC ANGTCCTTGG GGCACCCTGN   781 AAACATCTGA TGGTTCTCTA GAAACTGCTG ANGGCTGGNN CGCATCNGGG GACCATCTGT   841 TCTTGNNCCT GANNNGGGGC ANGAACTGCT NACCNCANAT ATCCNGTTTG GCCCATATTN   901 NGCTGTTCCA TCTGNNCTTG GCCCTGANCC NGGGNNNNNA NNNCNTNNCN NANATATNCN 961 GNTTNGNNCN NNNNNNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19013_B1Ecd.seq
>D07716B1_B1Ec_2_lacIpro-B_F_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNTCNG TGAGCGAGGA ANCGGAANAN CGCCCAATAC    61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT   121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG   181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG   241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA   301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG   361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC   421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC   481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT   541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA   601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT   661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT   721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA   781 AATAAAATCT TTTATTTTAT CGTCGANCAC TGTGCTGGCG GCCGCTTAAA AAATGCCTCT   841 TCATGTGTAA NGCGANGTGG TCCGANNNGN AAAATGNTCG GTCGCATTTT TTGGCACTGG   901 ANCGGGCGGN GCCCCNNGNG NTTACGGNAN NGCCNGGNCA GTTCANCNGA NCGGGNNNNT 961 TTCCNTNNNN NGCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

GNP_filter3_RDBDEP_html_230702.pl