Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(R479A)

Retroviral expression vector of human KLF4, mutant R479A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19015_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region, insert 5'(3xFLAG,hKLF4(R479A) 5') Sequence file: RDB19015_B1Eca.seq
>D07716B3_B1Ec_2_MMLV-pol_F_B06_06_ABI24.ab1     1 NNNNNNNNNN NANNNNNNGG NATCGNANCT TGGNNNCNCG CCGCCCACGT GAAGGCTGCC    61 GACCCCGGGG GTGGANCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA   121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG   181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT   241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA   301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC   361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG   421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG   481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG   541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC   601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG   661 GCGTGGCGCC GGGCGGCACG GGCGGANGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC   721 CGACGGCTCC CTTCAACCTG NCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG   781 CCGANCTCCT GCGGCCAGAA TTGGANCCGG TGTACATTCC GCCGCANCAG CCGCANCCGC   841 CAGGTGGCGG GCTGATGGGN CAAGTTCGTG CTGAAGGCGN CGCTGANCGC CCCNGGCANC   901 GNNTACNGNN GCCNGNCNNN CATCAGCNNC AGCNAAGGNA GNCNNNNNNG NNNNCNACCN 961 NGNGGNNGNN GNNNNNNNNN N //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4(R479A) 3') Sequence file: RDB19015_B1Ecb.seq
>D07716B3_B1Ec_2_pMXs-3LTRup_R2_A06_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCGTCGN NNNCTNNGCT GGCGGCCGCT TAAAAATGCC    61 TCTTCATGTG TAAGGCGAGG TGGTCCGACC TGGAAAATGC TCGGTCGCAT TTTTGGCACT   121 GGAACGGGCG GTGCCCCGTG TGTTTACGGT AGTGCGCGGT CAGTTCATCT GAGCGGGCGA   181 ATTTCCATCC ACAGCCGTCC CAGTCACAGT GGTAAGGTTT CTCACCTGTG TGGGTTCGCA   241 GGTGTGCCTT GAGATGGGAA CTCTTTGTGT AGGTTTTGCC GCAGCCCGCG TAATCACAAG   301 TGTGGGTGGC GGTCCTTTTC CGGGGCCACG ATCGTCTTCC CCTCTTTGGC TTGGGCTCCT   361 CTGGCATGCA GGAACCGGGT GGCATGAGCT CTTGGTAATG GAGCGGCGGG ACTTGCGGCT   421 GCATCTGATC GGGCAGGAAG GATGGGTAAT TGGGCCCCGG GTGGGGATGG AAGCCGGGAG   481 GAAGCGGCAG GGCAGGGTGA CAGTCCCTGC TGCTCAGCAC TTCCTCAAGA CCCAGGGTCG   541 GGGTAGTCCT GCTGGGGAGC TGCCGCCCCA GGGGGAAGTC GTGTGCAGCC GGCCGGTGGC   601 CATTGCTGAN AGGGGGTCCA GCGCCCAAGT GGGTGCACGA AGAGACCGCC TCCTGCTTGA   661 TCTTGGGGCA CGTGCGCGGC GGCCCGCCGT TGTANGGCGC CACCACNACC GGGTGGCTGC   721 CGTCAGGGCT GCCTTTGCTG ACGCTGATGA CCGACGGGCT GCCGTANTCG CTGCCAGGGG   781 CGCTCAGCGA CGCCTTCAGC ACGAACTTGC CCATCAGCCC GCCACCTGGC GGCTGCNGCT   841 GCTGCGGCNG AATGTACACC GNGTCCNNTT CTGGCCGCNN NNNNNNGCCN CNAANCCGCC   901 CGANGGGCTN ACGNNNTTNA TGTNCNNNNN NTTGNANGGN NNCCNNNNGA NGGGGANNNN 961 NNTNCNNGNC NNANNNGNNN NNNNCGNNCN NNNNNNCNNN NNNNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19015_B1Ecc.seq
>D07716B3_B1Ec_2_gag-N_R_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNNNTTTT NNGACNAATA CNGAAACNCA GTCAGACAGA    61 GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC GCGGCTTCGG TCCCAAACCG   121 AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT CTCGTCTCCT ACCAGAACCA   181 CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA ACGATCGGGA TTTTTGGACT   241 CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC GTCTCCCAGG GTTGCGGCCG   301 GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA TACAGAGCTA GTTAGCTAAC   361 TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA GACAATCGGA CAGACACAGA   421 TAAGTTGCTG GCCAGCTTAC CTCCCGGTGG TGGGTCGGTG GTCCCTGGGC AGGGGTCTCC   481 CGATCCCGGA CGAGCCCCCA AATGAAAGAC CCCCGCTGAC GGGTAGTCAA TCACTCAGAG   541 GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC AACTGCAAGA GGGTTTATTG   601 GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT GGCGCGCCGA GTGAGGGGTT   661 GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA GAAGCGAACT   721 GATTGGTTAG TTCAAATAAG GCACAGGGTC ATTTCANGTC CTTNGGGCAC CCTGGAAACA   781 TCTGATGGTT CTCTAGAAAC TGCTGANGGC TGGACCGCAT CTGGGGACNA TCTGTTCTTG   841 GCCCTGANCC GGGGCANGAA NTGCTTANCA CAGATATCCN GTTTGGCCCN TATTCAGCTG   901 TNNCATCTGN NCNTGNNCNN NANCCNGGGN NNNANNNGNN TANNNNANNN NTCCNNNTNN 961 GNCNNTATTC NGCTNNTNNN NNNNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19015_B1Ecd.seq
>D07716B3_B1Ec_2_lacIpro-B_F_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG CANCGNNNCN GTGNNCGANG AAGCGGAAGA NCGCCCAATA    61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG   121 TAAATACTTA AGAAAAAAAA CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC   181 GCTGACGGGT AGTCAATCAC TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC   241 GGATGCAACT GCAAGAGGGT TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG   301 AGGACTGGCG CCCCGAGTGA GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC   361 GCGCGAACAG AAGCGAGAAG CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT   421 CAGGTCCTTG GGGCACCCTG GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA   481 CCGCATCTGG GGACCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA   541 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA   601 ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT   661 TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTTCCAT GCCTTGCAAA ATGGCGTTAC   721 TTAAGCTAGC TTGCCAAACC TACAGGTGGG GTCTTTCATT CNCCCCTTTT TCTGGAGACT   781 AAATAAAATC TTTTATTTTA TCGTCNACCA CTGTGCTGGC GGCCGCTTAA AAATGCCTCT   841 TCATGTGTAA GGNGAGGTGG TCCNANCTGN AAANNGCTCG GTCGCNTTTT TGGNACTGGA   901 ACGGGCGGNG CCCCNNGTGT TTANGGTAGT GCGCGGNCAG TTCATCTGAN CNGGCGNANT 961 TTCCNTNCNN NNNCNNNNCC NNNCNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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