Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(S500A)

Retroviral expression vector of human KLF4, mutant S500A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19019_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(S500A) 5') Sequence file: RDB19019_B1Eca.seq
>D07716B7_B1Ec_1_MMLV-pol_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG NNTCGCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC    61 GACCCCGGGG GTGGANCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA   121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG   181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT   241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA   301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC   361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG   421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG   481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG   541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC   601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG   661 GCGTGGCGCC GGGCGGCACG GGCGGANGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC   721 CGACGGCTCC CTTCAACCTG GCGGANATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG   781 CCGANCTCCT GCGGCCAGAA TTGGACCCGG TGTACATTCC GCCGCAGCAG CCGCAGCCGC   841 CAGGTGGCGG GCTGATGGGC AAGTTCGTGC TGAANGCGTC GCTGANCGNC CCTGGCAGCG   901 AGNACGGCAG CCCGNNNNNC ATCAGCGNCA GCAAANGCAG CCCNGANNGN AGNCACCCNG   961 NNGGTGGTGG CGCCCTANAN NNGCNGNNNG NCGCNCANNN NCCCNANNTN NANNNNNANG 1021 NNGNTNTNNN NNNGNNNNNN NNNN //
Primer: hKLF4-mut check_R2 (Pr1009) Region: insert 3'(hKLF4(S500A) 3',3' LTR Sequence file: RDB19019_B1Ecb.seq
>D07716B7_B1Ec_1_hKLF4-mut check_R2_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNNN NNNNNNCCCG GGGCCCNATT ACCCATCCTT    61 CCTGCCCGAT CAGATGCAGC CGCAAGTCCC GCCGCTCCAT TACCAAGAGC TCATGCCACC   121 CGGTTCCTGC ATGCCAGAGG AGCCCAAGCC AAAGAGGGGA AGACGATCGT GGCCCCGGAA   181 AAGGACCGCC ACCCACACTT GTGATTACGC GGGCTGCGGC AAAACCTACA CAAAGAGTTC   241 CCATCTCAAG GCACACCTGC GAACCCACAC AGGTGAGAAA CCTTACCACT GTGACTGGGA   301 CGGCTGTGGA TGGAAATTCG CCCGCTCAGA TGAACTGACC AGGCACTACC GTAAACACAC   361 GGGGCACCGC CCGTTCCAGT GCCAAAAATG CGACCGAGCA TTTGCCAGGT CGGACCACCT   421 CGCCTTACAC ATGAAGAGGC ATTTTTAAGC GGCCGCCAGC ACAGTGGTCG ACGATAAAAT   481 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG   541 GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG   601 AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT   661 CTGTGGTAAG CAGTTCCTGC CCCGGCTCAN GGCCAAGAAC AGATGGAACA GCTGAATATG   721 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCANGGCCN AGAACAGATG   781 GTCCCCAGAT GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA NCATCAGANG TTTCCAGGGT   841 GCCCCAAGGA CCTGAAATGA NCCTGTGCCT TANTTGAACT AACCAATCAN TTCGCTNNNC   901 GCTNNNGTNC NCGNGCTTCT GCTCCNNNAN CNCNATAAAA NANCCNNNAA NNNNNNNNNC 961 GGGNNNNCNN NNNNNNNANN GNNNNANNNN NNNNGNNNNN NNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4(S500A) 3' Sequence file: RDB19019_B1Ecc.seq
>D07716B7_B1Ec_1_pMXs-3LTRup_R2_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTTNNTCG TCGANNNCTG TGCTGNNCGG CCGCTTAAAA    61 ATGCCTCTTC ATGTGTAAGG CGAGGTGGTC CGACCTGGCA AATGCTCGGT CGCATTTTTG   121 GCACTGGAAC GGGCGGTGCC CCGTGTGTTT ACGGTAGTGC CTGGTCAGTT CATCTGAGCG   181 GGCGAATTTC CATCCACAGC CGTCCCAGTC ACAGTGGTAA GGTTTCTCAC CTGTGTGGGT   241 TCGCAGGTGT GCCTTGAGAT GGGAACTCTT TGTGTAGGTT TTGCCGCAGC CCGCGTAATC   301 ACAAGTGTGG GTGGCGGTCC TTTTCCGGGG CCACGATCGT CTTCCCCTCT TTGGCTTGGG   361 CTCCTCTGGC ATGCAGGAAC CGGGTGGCAT GAGCTCTTGG TAATGGAGCG GCGGGACTTG   421 CGGCTGCATC TGATCGGGCA GGAAGGATGG GTAATTGGGC CCCGGGTGGG GATGGAAGCC   481 GGGAGGAAGC GGCAGGGCAG GGTGACAGTC CCTGCTGCTC AGCACTTCCT CAAGACCCAG   541 GGTCGGGGTA GTCCTGCTGG GGAGCTGCCG CCCCAGGGGG AAGTCGTGTG CAGCCGGCCG   601 GTGGCCATTG CTGAGAGGGG GTCCAGCGCC CAAGTGGGTG CACGAAGAGA CCGCCTCCTG   661 CTTGATCTTG GGGCACGTGC GCGGCGGCCC GCCGTTGTAG GGCGCCACCA CCACCGGGTG   721 GCTGCCGTCA GGGCTGCCTT TGCTGACGCT GATGACCGAC GGGCTGCCGT ACTCGCTGCC   781 AGGGGCGCTC NNCGACGCCT TCNGCACGAA CTTGCCCATC AGCCCGCCNC CTGGCGGCTG   841 CGGCTGCTGC GGCGGANTGT ACACCGGGGN CCNATTCTGG NNCGCANGAN CTCGGCNNCN   901 ANNNCNNCCG ANGGGCTCAC GTCGTTGATG TCCGCCAGGN TGAANGGAGC CGTCGGANGG   961 GGANCGNANN NCCTGCNNTA NANGANNNNT CCGCCCGTGN NNCNNGCGCC ACGCCCNGGN  1021 NNNNNCCNGN CCNNATNNGN NNNNGTGNAN NNNCNNNNNN NNGGNNNNCN NGNNNNGNNN 1081 NNNNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19019_B1Ecd.seq
>D07716B7_B1Ec_1_gag-N_R_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NTTNNNNNAC NAAATACAGA AACACAGTCN    61 GACAGAGACA ACACAGAACG ATGCTGCAGC AGACAAGACG CGCGGCGCGG CTTCGGTCCC   121 AAACCGAAAG CAAAAATTCA GACGGAGGCG GGAACTGTTT TAGGTTCTCG TCTCCTACCA   181 GAACCACATA TCCCTCCTAT TAGGGGGGTG CACCAAAGAG TCCAAAACGA TCGGGATTTT   241 TGGACTCAGG TCGGGCCACA AAAACGGCCC CCGAAGTCCC TGGGACGTCT CCCAGGGTTG   301 CGGCCGGGTG TTCCGAACTC GTCAGTTCCA CCACGGGTCC GCCAGATACA GAGCTAGTTA   361 GCTAACTAGT ACCGACGCAG GCGCATAAAA TCAGTCATAG ACACTAGACA ATCGGACAGA   421 CACAGATAAG TTGCTGGCCA GCTTACCTCC CGGTGGTGGG TCGGTGGTCC CTGGGCAGGG   481 GTCTCCCGAT CCCGGACGAG CCCCCAAATG AAAGACCCCC GCTGACGGGT AGTCAATCAC   541 TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC GGATGCAACT GCAAGAGGGT   601 TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG AGGACTGGCG CGCCGAGTGA   661 GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC GCGCGAACAG AAGCGAGAAG   721 CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT CAGGTCCTTG GGGCANCCTG   781 GAAACATCTG ATGGTTCTCT ANAAACTGCT GANGGCTGGA CCGCATCTGG GGANCATCTG   841 TTCTTGGNCC TGANCCGGGG CANGAACTGC TTACCACAGA TATCCNGTTN GGNCCANNTT   901 CNGCTGTTCC ATCTGTTCTT GGCCCTGANC CGGGGCANGA NNNGCTNACC NCAGATATCC   961 TGNTNNNNCC ATATTCAGCT GTTCNNTCTG TNCCNGANCN NGNNCTGNAN TTTTCNATTC 1021 TCNNNNATGN NNTTTNNNNN NNCNNNNNNA ANNNGNNNNN NNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19019_B1Ece.seq
>D07716B7_B1Ec_1_lacIpro-B_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCGNN NNNNTGAGCG AGGNNNCGGA AGAGCGCCCA    61 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAACTA TGGCCATTTA   121 ATGTAAATAC TTAAGAAAAA AAACCAAATT AATTTTGATA CATGCTGCAT GTGAAAGACC   181 CCCGCTGACG GGTAGTCAAT CACTCAGAGG AGACCCTCCC AAGGAACAGC GAGACCACAA   241 GTCGGATGCA ACTGCAAGAG GGTTTATTGG ATACACGGGT ACCCGGGCGA CTCAGTCAAT   301 CGGAGGACTG GCGCCCCGAG TGAGGGGTTG TGGGCTCTTT TATTGAGCTC GGGGAGCAGA   361 AGCGCGCGAA CAGAAGCGAG AAGCGAACTG ATTGGTTAGT TCAAATAAGG CACAGGGTCA   421 TTTCAGGTCC TTGGGGCACC CTGGAAACAT CTGATGGTTC TCTAGAAACT GCTGAGGGCT   481 GGACCGCATC TGGGGACCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC TGCTTACCAC   541 AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CTTGGCCCTG AGCCGGGGCA   601 GGAACTGCTT ACCACAGATA TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCCTGA   661 CCTTGATCTG AACTTCTCTA TTCTCAGTTA TGTATTTTTC CATGCCTTGC AAAATGGCGT   721 TACTTAAGCT AGCTTGCCAA ACCTACAGGT GGGGTCTTTC ATTCCCCCCT TTTTCTGGAN   781 ACTAAATAAA ATCTTTTATT TTATCGTCGA NCACTGTGCT GGCGGCCGCT TAAAAATGCC   841 TCTTCATGTG TAANNNGANG TGGTCCGANC TGGCAAATGC TCGGTCGCAT TTTTGNNNCT   901 GGANNGGGCG GNGNCCCGTG TGTTTACGGN ANNGCCNNNN NNNNTCNTCT GANCGGGCNA   961 NNTTNNNTCC ACAGNNNNNC CNNTCACNNN GGNNNNNTTT CNCNNCTGTG TGGGNTNNCN 1021 NNNTGNCNTN GANANNNNNN NNCNTTNNNN NNNNTTNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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