Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(D503A)

Retroviral expression vector of human KLF4, mutant D503A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19021_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(D503A)5') Sequence file: RDB19021_B1Eca.seq
>D07716B9_B1Ec_3_MMLV-pol_F_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCGNNN NCTTGGNNAC NCGCCGCCCN CGTGAAGGCT    61 GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCGGATC TAGCTAGTTA ATTAAGGATC   121 CCAGTGTGGT GGTACGGGAA TTCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC   181 ATGACATCGA CTACAAGGAT GACGATGACA AGGCTGTCAG CGACGCGCTG CTCCCATCTT   241 TCTCCACGTT CGCGTCTGGC CCGGCGGGAA GGGAGAAGAC ACTGCGTCAA GCAGGTGCCC   301 CGAATAACCG CTGGCGGGAG GAGCTCTCCC ACATGAAGCG ACTTCCCCCA GTGCTTCCCG   361 GCCGCCCCTA TGACCTGGCG GCGGCGACCG TGGCCACAGA CCTGGAGAGC GGCGGAGCCG   421 GTGCGGCTTG CGGCGGTAGC AACCTGGCGC CCCTACCTCG GAGAGAGACC GAGGAGTTCA   481 ACGATCTCCT GGACCTGGAC TTTATTCTCT CCAATTCGCT GACCCATCCT CCGGAGTCAG   541 TGGCCGCCAC CGTGTCCTCG TCAGCGTCAG CCTCCTCTTC GTCGTCGCCG TCGAGCAGCG   601 GCCCTGCCAG CGCGCCCTCC ACCTGCAGCT TCACCTATCC GATCCGGGCC GGGAACGACC   661 CGGGCGTGGC GCCGGGCGGC ACGGGCGGAG GCCTCCTCTA TGGCAGGGAG TCCGCTCCCC   721 CTCCGACGGC TCCCTTCAAC CTGGCGGACA TCAACGACGT GAGCCCCTCG GGCGGCTTCG   781 TGGNCGAGCT CCTGCGGNCA GANNTGNANC CGGTGTACAT TCCGCCGCAG CAGCCGCAGC   841 CGCCAGGTGG CGGGCTGATG GGCAAGTTCG TGCTGAAGGN GTCGCTGANC GCCCCTGGCA   901 NCGAGTACNG GCAGCCNNTC NGNCATCAGC GTCANNNAAG GNANNCNTGA NGGCANCCAC   961 CCGGNGGTGG NGGNGCCCTA NNACGGNGGN NNNNNGCGCN NGTNCCCCNN NANNANNCNN  1021 NANNNNGNNN CNTNNNGNNN CCNNNTTGGN NNNNTNNNNC CCNTCNCNNN NANNNGNNNN 1081 NNNNNN //
Primer: hKLF4-mut check_R2 (Pr1009) Region: insert 3'(hKLF4(D503A) 3'),3' LTR Sequence file: RDB19021_B1Ecb.seq
>D07716B9_B1Ec_3_hKLF4-mut check_R2_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCNGGGGNCC NATTACCCAT CCTTCCTGCC    61 CGATCAGATG CAGCCGCAAG TCCCGCCGCT CCATTACCAA GAGCTCATGC CACCCGGTTC   121 CTGCATGCCA GAGGAGCCCA AGCCAAAGAG GGGAAGACGA TCGTGGCCCC GGAAAAGGAC   181 CGCCACCCAC ACTTGTGATT ACGCGGGCTG CGGCAAAACC TACACAAAGA GTTCCCATCT   241 CAAGGCACAC CTGCGAACCC ACACAGGTGA GAAACCTTAC CACTGTGACT GGGACGGCTG   301 TGGATGGAAA TTCGCCCGCT CAGATGAACT GACCAGGCAC TACCGTAAAC ACACGGGGCA   361 CCGCCCGTTC CAGTGCCAAA AATGCGACCG AGCATTTTCC AGGTCGGCCC ACCTCGCCTT   421 ACACATGAAG AGGCATTTTT AAGCGGCCGC CAGCACAGTG GTCGACGATA AAATAAAAGA   481 TTTTATTTAG TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC   541 TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT   601 TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG   661 TAAGCAGTTC CTGCCCCGGC TCANGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA   721 ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCANN GCCAAGAACA GATGGTCCCC   781 ANATGCGGNC CAGCCCTCAN CAGTTTCTAG AGAACCATCA NATGTTTCCA GNGTGCCCCA   841 AGGANCTGAA ATGANCCTGT GCCTTATTTG AACTAACNNA TCAGTTCGCT TCTCGCTTCN   901 GTNNNCGCGC TNNTGCTCCC CNANCTNNAN NAAANANCCC ACNNNCCCTC ACTNNGGGGC   961 GNCNNNNCNN NNNNNGANTG ANNNNNNNNG NNNNCCNNNN NNNCNNNNAA NNCNNNNNNN 1021 NNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert mid(hKLF4(D503A) mid) Sequence file: RDB19021_B1Ecc.seq
>D07716B9_B1Ec_3_pMXs-3LTRup_R2_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCGNNNNNN GTGCTNNGCG GCCGCTTAAA    61 AATGCCTCTT CATGTGTAAG GCGAGGTGGG CCGACCTGGA AAANGNTCGG TCGCATTTTT   121 GGCACTGGAA CGGGCGGTGC CCCGTGTGTT TACGGTAGTG CCTGGTCAGT TCATCTGAGC   181 GGGCGAATTT CCATCCACAG CCGTCCCAGT CACAGTGGTA AGGTTTCTCA CCTGTGTGGG   241 TTCGCAGGTG TGCCTTGAGA TGGGAACTCT TTGTGTAGGT TTTGCCGCAG CCCGCGTAAT   301 CACAAGTGTG GGTGGCGGTC CTTTTCCGGG GCCACGATCG TCTTCCCCTC TTTGGCTTGG   361 GCTCCTCTGG CATGCAGGAA CCGGGTGGCA TGAGCTCTTG GTAATGGAGC GGCGGGACTT   421 GCGGCTGCAT CTGATCGGGC AGGAAGGATG GGTAATTGGG CCCCGGGTGG GGATGGAAGC   481 CGGGAGGAAG CGGCAGGGCA GGGTGACAGT CCCTGCTGCT CAGCACTTCC TCAAGACCCA   541 GGGTCGGGGT AGTCCTGCTG GGGAGCTGCC GCCCCAGGGG GAAGTCGTGT GCAGCCGGCC   601 GGTGGCCATT GCTGAGAGGG GGTCCAGCGC CCAAGTGGGT GCACGAAGAG ACCGCCTCCT   661 GCTTGATCTT GGGGCACGTG CGCGGCGGCC CGCCGTTGTA GGGCGCCACC ACCACCGGGT   721 GGCTGCCGTC AGGGCTGCCT TTGCTGACGC TGATGACCGA CGGGCTGCCG TACTCGCTGC   781 CNNNGGCGCT CAGCGACGCC TTCAGCACGA ACTTGCCCAT CAGCCCGCCA CCTGGCGGCT   841 GCGGCTGCTG CGGNGNAATG TNNACCNGGT CNNATTCTGG NCGNNNNGAN CTCGGNCNNC 901 NNNNNNNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19021_B1Ecd.seq
>D07716B9_B1Ec_3_gag-N_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNNNNAATT TTCNNANNAA TACNGAAACA CAGTCAGACA    61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC   121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC   181 CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA   241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC   301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA   361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA   421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT   481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG   541 AGGAGACCCT CCCAAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT   601 TGGATACACG GGTACCCGGG CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG   661 TTGTGGGCTC TTTTATTGAG CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA   721 CTGATTGGTT AGTTCAAATA AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGAAA   781 CATCTGATGG TTCTCTAGAA ACTGCTGANG GCTGGACCGC ATCTGGGGAN CATCTGTTCT   841 TGGCCCTGAG CCGGGGCANG AACTGCTTAN CACAGATATC CTGTTTGGNC CATATTCNGC   901 TGTTCCNTCT GNNCTTGGNC CNGANCCGGG GCNNNNANNG CTNACCACAG ATATCCNGNT   961 NGGNCCATAT TNNGCNGTTN CNTCTGNTNN NGANNNNGNN NNGNNNTTTT CTATTNNCAN 1021 NNNTGNNNTT TNNNNNNNNN N //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19021_B1Ece.seq
>D07716B9_B1Ec_3_lacIpro-B_F_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGC ANCGNGTCNG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC    61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT   121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG   181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG   241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA   301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG   361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC   421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC   481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT   541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA   601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT   661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT   721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA   781 AATAAAATCT TTTATTTTAT CGTCGACCAC TGTGCTGGCG GCCGCTTAAA AATGCCTCTT   841 CATGTGTAAG GNGAGGTGGG CCGACCTGNA AAATGCTCGG TCGCATTTTT GGNACTGGAA   901 CGGGCGGNGC CCCGTGTGTT TACGGNAGTG NCNNNNCAGT TCATCTGANC GGGCGAATTT   961 CCATCCNCAN NNNTCCCANN CACAGNGNNN NNNNTTCNCN NNNGNNTGGG NTCGCNNGNG  1021 NGNCNTNNAN ANGGGNNCNN NTNNNNNNNN NTTNNNNNNN NNCNNNNNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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