Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(H504A)

Retroviral expression vector of human KLF4, mutant H504A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19022_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(H504A) 5') Sequence file: RDB19022_B1Eca.seq
>D07716C1_B1Ec_3_MMLV-pol_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGNNTCGNA NCTTGGNNAC NCGCCGCCCN CGTGAAGGCT    61 GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCGGATC TAGCTAGTTA ATTAAGGATC   121 CCAGTGTGGT GGTACGGGAA TTCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC   181 ATGACATCGA CTACAAGGAT GACGATGACA AGGCTGTCAG CGACGCGCTG CTCCCATCTT   241 TCTCCACGTT CGCGTCTGGC CCGGCGGGAA GGGAGAAGAC ACTGCGTCAA GCAGGTGCCC   301 CGAATAACCG CTGGCGGGAG GAGCTCTCCC ACATGAAGCG ACTTCCCCCA GTGCTTCCCG   361 GCCGCCCCTA TGACCTGGCG GCGGCGACCG TGGCCACAGA CCTGGAGAGC GGCGGAGCCG   421 GTGCGGCTTG CGGCGGTAGC AACCTGGCGC CCCTACCTCG GAGAGAGACC GAGGAGTTCA   481 ACGATCTCCT GGACCTGGAC TTTATTCTCT CCAATTCGCT GACCCATCCT CCGGAGTCAG   541 TGGCCGCCAC CGTGTCCTCG TCAGCGTCAG CCTCCTCTTC GTCGTCGCCG TCGAGCAGCG   601 GCCCTGCCAG CGCGCCCTCC ACCTGCAGCT TCACCTATCC GATCCGGGCC GGGAACGACC   661 CGGGCGTGGC GCCGGGCGGC ACGGGCGGAN GCCTCCTCTA TGGCAGGGAG TCCGCTCCCC   721 CTCCGACGGC TCCCTTCNAC CTGGCGGANA TCAACGACGT GAGCCCCTCG GGCGGNTTCN   781 TGGCCNANCT CCTGCGGCCN GAATTGGANC CGGNGTACNT TCCGCCGCAN CAGCCGCAGC   841 CNNCAGGTGG CGGGCTGATG GGCAAGTTCG TGCTGAANGN GTCNCTGANC GCCCCTGNNN   901 NNNAGNANGG NAGCCCGNCN GNCATCAGCG NCNNCAAAGG NAGNCCTGAN NNNNNNNNCC   961 CNGNNNNGGN GGCGCCCNAN ANCNGCGGGN CNNCNCGNNC GTGCCNCNNG NTCANNCNNN  1021 NNNNNGNNNN TNNNNGNNCC NNCNTNGNNN NNNGNNNCCC NNNNCNNNNN NGNNNNNGNN 1081 NNGNNNNNNN NNN //
Primer: hKLF4-mut check_R2 (Pr1009) Region: insert 3'(hKLF4(H504A) 3'),3' LTR Sequence file: RDB19022_B1Ecb.seq
>D07716C1_B1Ec_3_hKLF4-mut check_R2_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNT NNNNNNNNCC GGGGCCCNNT TACCCATCCT    61 TCCTGCCCGA TCAGATGCAG CCGCAAGTCC CGCCGCTCCA TTACCAAGAG CTCATGCCAC   121 CCGGTTCCTG CATGCCAGAG GAGCCCAAGC CAAAGAGGGG AAGACGATCG TGGCCCCGGA   181 AAAGGACCGC CACCCACACT TGTGATTACG CGGGCTGCGG CAAAACCTAC ACAAAGAGTT   241 CCCATCTCAA GGCACACCTG CGAACCCACA CAGGTGAGAA ACCTTACCAC TGTGACTGGG   301 ACGGCTGTGG ATGGAAATTC GCCCGCTCAG ATGAACTGAC CAGGCACTAC CGTAAACACA   361 CGGGGCACCG CCCGTTCCAG TGCCAAAAAT GCGACCGAGC ATTTTCCAGG TCGGACGCCC   421 TCGCCTTACA CATGAAGAGG CATTTTTAAG CGGCCGCCAG CACAGTGGTC GACGATAAAA   481 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT   541 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA   601 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA   661 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA CAGATGGAAC AGCTGAATAT   721 GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCANGGCC AAGAACAGAT   781 GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG TTTCTAGAGA ACCATCANAN GTTTNCNGGG   841 NGCCCCNAGG ACCTGAAATG ANCCTGTGCC NTATTTGAAC TANCCNANCA NTTCGCTTCN   901 CGCTTCTGTT CNCGCGCTNC NGNNCCCCNA NNTNNNTAAA AGANCCNNNN ACCCNNCACT 961 CNGGGNNNNC NNNNNNNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert mid(hKLF4(H504A) mid) Sequence file: RDB19022_B1Ecc.seq
>D07716C1_B1Ec_3_pMXs-3LTRup_R2_B08_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCGTCN NNNNCTGNGC TGGCGGCCGC TTAAAANTGC    61 CTCTTCNTGT GTAANGCGAG GGCGTCCGAC CTGGAAAATG CTCGGTCGCA TTTTTGGCAC   121 TGGAACGGGC GGTGCCCCGT GTGTTTACGG TAGTGCCTGG TCAGTTCATC TGAGCGGGCG   181 AATTTCCATC CACAGCCGTC CCAGTCACAG TGGTAAGGTT TCTCACCTGT GTGGGTTCGC   241 AGGTGTGCCT TGAGATGGGA ACTCTTTGTG TAGGTTTTGC CGCAGCCCGC GTAATCACAA   301 GTGTGGGTGG CGGTCCTTTT CCGGGGCCAC GATCGTCTTC CCCTCTTTGG CTTGGGCTCC   361 TCTGGCATGC AGGAACCGGG TGGCATGAGC TCTTGGTAAT GGAGCGGCGG GACTTGCGGC   421 TGCATCTGAT CGGGCAGGAA GGATGGGTAA TTGGGCCCCG GGTGGGGATG GAAGCCGGGA   481 GGAAGCGGCA GGGCAGGGTG ACAGTCCCTG CTGCTCAGCA CTTCCTCAAG ACCCAGGGTC   541 NNNNNNGTCC TGCTGGGGAG CTGCCGCCCC AGGGGGAAGT CGTGTGCAGC CGGCCGGTGG   601 CCATTGCTGA GAGGGGGTCC AGCGCCCAAG TGGGTGCACG AAGAGACCGC CTCCTGCTTG   661 ATCTTGGGGC ACGTGCGCGG CGGCCCGCCG NTGTANGGCG CCACCACCAC CGGGTGGCTG   721 CCGTCAGGGC TGCCTTTGCT GANGCTGATG ACCGACGGGC TGCCGTACTC GCTGCCAGGG   781 GCGCTCAGCG ACGCCTTCAG CACGAACTTG CCCNATCAGC CCGCCACCTG NCGGCTGCGG   841 CTGCTGCNGC GNAATGTACA CCNGGGTCCA ATTNTGGCCG CANGANCNCG GCCNCGANNC   901 CGCCNNANGG GGCNCACNNC NNTNNANGTC CGCCANGNNN GAANNNNANC NNNNNGNNNG 961 GGGNNCNNNN NNCCNNNNNN NNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19022_B1Ecd.seq
>D07716C1_B1Ec_3_gag-N_R_C08_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TTNNGANNAA TANCNGAAAN CACAGTCAGA    61 CAGANACNNA CACAGAACGN NNNNNTGCAG CAGACAAGAC GCGCGGCGCG GCTTCGGTCC   121 CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT TTAGGTTCTC GTCTCCTACC   181 AGAACCACAT ATCCCTCCTA TTAGGGGGGT GCACCAAAGA GTCCAAAACG ATCGGGATTT   241 TTGGACTCAG GTCGGGCCAC AAAAACGGCC CCCGAAGTCC CTGGGACGTC TCCCAGGGTT   301 GCGGCCGGGT GTTCCGAACT CGTCAGTTCC ACCACGGGTC CGCCAGATAC AGAGCTAGTT   361 AGCTAACTAG TACCGACGCA GGCGCATAAA ATCAGTCATA GACACTAGAC AATCGGACAG   421 ACACAGATAA GTTGCTGGCC AGCTTACCTC CCGGTGGTGG GTCGGTGGTC CCTGGGCAGG   481 GGTCTCCCGA TCCCGGACGA GCCCCCAAAT GAAAGACCCC CGCTGACGGG TAGTCAATCA   541 CTCAGAGGAG ACCCTCCCAA GGAACAGCGA GACCACAAGT CGGATGCAAC TGCAAGAGGG   601 TTTATTGGAT ACACGGGTAC CCGGGCGACT CAGTCAATCG GAGGACTGGC GCGCCGAGTG   661 AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG GGAGCAGAAG CGCGCGAACA GAAGCGAGAA   721 GCGAACTGAT TGGTTAGTTC AAATAAGGCA CANGGTCATT TCANGTCCTT GGGGCANCCT   781 GGAANCATCT GATGGTTCTC TANAAACTGC TGANGGCTGG ACCGCATCNG GGGGANCATC   841 TGTTCTTGGC CNNGANNCNG GGGCANGAAC TGCNTNACCA NNGATATCCN GNTTNGNNCN   901 NTATTCNNCT NNNCCATCTG TTCNTNGNNC NNNANNNGGG NNNNNNNANN GCTNANNNCN 961 NNNNNNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19022_B1Ece.seq
>D07716C1_B1Ec_3_lacIpro-B_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN ANNNNNNNNN NNCNNNGAGC GAGGAAGCGG AAGANCGCCC    61 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAACT ATGGCCATTT   121 AATGTAAATA CTTAAGAAAA AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC   181 CCCCGCTGAC GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA   241 AGTCGGATGC AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA   301 TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG   361 AAGCGCGCGA ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC   421 ATTTCAGGTC CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC   481 TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA   541 CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGC   601 AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCCTG   661 ACCTTGATCT GAACTTCTCT ATTCTCAGTT ATGTATTTTT CCNTGCCTTG CAAAATGGCG   721 TTACTTAAGC TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTCCCCCC TTTTTCTGGA   781 GACTAAATNA AANTCTTTTA TTTTATCGTC NACCACTGNG CTGGCGGNNN CTTAAAAATG   841 CCTCTTCATG TGTANGCGAG GGCNNNCGAC CTNNNAATGN TCGGTCGNNT TTTTGNNACT   901 GNAACGGGCG GNGCCCCNNG NGTTTACGGN AGNGNCTGGN CAGTTCNTCN NANCGGGNCN   961 AATTTNCNTC CNNANNCGTC CCANNCNCAG NGGNAANGGN TNNTCNNCNG TGNGGGNTCN  1021 NNNNNGNNNC NTGNNNANNG GANNNNNNNT NNGNNNNTTT TGNNNNNNNC CNNNNNNANN  1081 NNNNANNGNG GNNNGNNGGT NNNTTTNCNN GGNNNNNNNN NCNNNNNNNN CCNNNNNNTT 1141 NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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