Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(L507R)

Retroviral expression vector of human KLF4, mutant L507R.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19024_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(L507R) 5') Sequence file: RDB19024_B1Eca.seq
>D07716C3_B1Ec_1_MMLV-pol_F_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNGNNGG NNTCGNAGCT TGGNNACNCG CCGCCCNCGT GAAGGCTGCC    61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA   121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG   181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT   241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA   301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC   361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG   421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG   481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG   541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC   601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG   661 GCGTGGCGCC GGGCGGCACG GGCGGAGGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC   721 CGACGGCTCC CTTCAACCTG GCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG   781 NCGANCTCCT GCGGCCAGAA TTGGACCCGG NGTACATTCC GCCGCANCAG CCGCAGCCGC   841 CNGGTGGCGG GCTGATGGGC AANTTCGTGC TGAANGNGTC GCTGANCGCC CCTGGCAGCG   901 AGTACNGNAG CCCGTCNGNC NTCAGCGNCA GNNAAGGNAG NCCTGANGGC AGCCNCNNGN   961 NGGNGGNGGC NCCNNACANC NGNGGGNCNN NNNGCNNGNG CCCNNNGATN NANNNNNNNN  1021 GNNNNNNNCT TNNNGNNNCC NNNNGGNNNN NNGNNCCCCN NNNCNNCNNN NGNNNNNNNN 1081 NN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(attB2,hKLF4(L507R)3') Sequence file: RDB19024_B1Ecb.seq
>D07716C3_B1Ec_1_pMXs-3LTRup_R2_B09_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNCGTCNNN TNNNNNAGTA CATAATGGNN TTCCTTACGC    61 GAAATACGGG CAGACATGGC CTGCCCGGTT ATTATTATTT TTGACACCAG ACCAACTGGT   121 AATGGTAGCG ACCGGCGCTC AGCTGGAATA TCACCACTTT GTACAAGAAA GCTGGGTCGG   181 CGCGCCCACC CTTTTAAAAA TGTCTCTTCA TGTGTCGGGC GAGGTGGTCC GACCTGGAAA   241 ATGCTCGGTC GCATTTTTGG CACTGGAACG GGCGGTGCCC CGTGTGTTTA CGGTAGTGCC   301 TGGTCAGTTC ATCTGAGCGG GCGAATTTCC ATCCACAGCC GTCCCAGTCA CAGTGGTAAG   361 GTTTCTCACC TGTGTGGGTT CGCAGGTGTG CCTTGAGATG GGAACTCTTT GTGTAGGTTT   421 TGCCGCAGCC CGCGTAATCA CAAGTGTGGG TGGCGGTCCT TTTCCGGGGC CACGATCGTC   481 TTCCCCTCTT TGGCTTGGGC TCCTCTGGCA TGCAGGAACC GGGTGGCATG AGCTCTTGGT   541 AATGGAGCGG CGGGACTTGC GGCTGCATCT GATCGGGCAG GAAGGATGGG TAATTGGGCC   601 CCGGGTGGGG ATGGAAGCCG GGAGGAAGCG GCAGGGCAGG GTGACAGTCC CTGCTGCTCA   661 GCACTTCCTC AAGANCCANG GTCGGGGTAG TCCTGCTGGG GAGCTGCCGC CCCANGGGGA   721 AGTCGTGTGC AGCCGGCCGG TGGCCATTGC TGANAGGGGG TCCAGCGCNC AAGNGGGTGC   781 ACGAAGANAN CGCCTCCTGC TNGATCTTGG GGCACGTGCG CGGCGGCCCG CCNTTGTNNG   841 GCGCCACCAC CACNNGGNGG NTGNCGTCNN GNCTGCCNTN GCTGANGCNN ANGANNNANN 901 GGNNNNNNNA NNNNNNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro,5' LTR,MMLV psi Sequence file: RDB19024_B1Ecc.seq
>D07716C3_B1Ec_1_Amp_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNTN ATNNGGGTTA TTGTCTCNTG AGCGGANACA    61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG   121 TGCCACCTGC ATAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC   181 ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAA AAGTTCAGAT CAAGGTCAGG   241 AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC   301 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT   361 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC   421 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC   481 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC   541 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATTGA   601 CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG   661 GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT   721 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC   781 CGGGAGGTAA NCTGGNCAGC AACTTATCTG TGTCTGTCCG ATNGTCTAGT GTCTATGACT   841 GANTTTATGC GCCTGCGTCG GTNCTAGTTA GCTAACTANC TCTGTATCTG GNGNANNNGT   901 GGTGGANCTG ACGANTNNGN AACACCCGNN NGCNNNCCNN NGNGANNTCC CNNGNNNTTC   961 NGGGGNNGTT TTTGNGGCCC GNNNNGANNC NAAAAATCCC GATCNNTTTG NNNNNTTNNN 1021 NGNNNNNNCC NTNNNNNNNN NN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19024_B1Ecd.seq
>D07716C3_B1Ec_1_gag-N_R_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNTTNNNAN NAATACNGAA ACACAGTCAG    61 ACAGAGACAA CACAGAACGA TGCTGCAGCA GACAAGACGC GCGGCGCGGC TTCGGTCCCA   121 AACCGAAAGC AAAAATTCAG ACGGAGGCGG GAACTGTTTT AGGTTCTCGT CTCCTACCAG   181 AACCACATAT CCCTCCTATT AGGGGGGTGC ACCAAAGAGT CCAAAACGAT CGGGATTTTT   241 GGACTCAGGT CGGGCCACAA AAACGGCCCC CGAAGTCCCT GGGACGTCTC CCAGGGTTGC   301 GGCCGGGTGT TCCGAACTCG TCAGTTCCAC CACGGGTCCG CCAGATACAG AGCTAGTTAG   361 CTAACTAGTA CCGACGCAGG CGCATAAAAT CAGTCATAGA CACTAGACAA TCGGACAGAC   421 ACAGATAAGT TGCTGGCCAG CTTACCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG   481 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCG CTGACGGGTA GTCAATCACT   541 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT   601 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC GCCGAGTGAG   661 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC   721 GAACTGATTG GTTAGTTCAA ATAANGCACA GGGTCATTTC NGGTCCTTGG GGCACCCTGN   781 AAACATCTGA TGGTTCTCTA GAAACTGCTG ANGGCTGGAC CGCATCTGGG GACCATCTGT   841 TCTTGGCCCT GAGCCGGGGC ANGAACTGCT NANNNCAGAT ATCCTGNTNG GNCCNTATNC   901 NNCTGTTCCN TCTGTTCTTG NNCNTGANCC NGGGNNNNNA ACTGCTTANC ACANATNTCC   961 NGNTNNNNCC ATATNCNGCN NNNCNNTCTG NNNNNGANNN NGATCNGAAC TTTNCNANNN 1021 TCNNTNANGN NTTTTTNNNN NNNTNNNNAA NNNNNNNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19024_B1Ece.seq
>D07716C3_B1Ec_1_lacIpro-B_F_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNCTA NNNNNNNNNT    61 NNNNTAAATA CNNNNNANNA AAAACCNNNT TAATTTTGAN ACNNGCTGCA TGTGAAAGAC   121 CCCCGCTGAC GGGTANTCAA TCACTCNNAG GAGACCCTCC CAAGGAACAG CGAGACCACA   181 NGTCGGATGC AACTGCNAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCANTCAA   241 TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAN   301 AANCGCGCGA ACNNNNNNGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC   361 ATTTCAGGTN NNNNNGNNNN NNTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC   421 TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA   481 CAGATATCCT GTTTGGCCCA TATTCNNCTG NNNNNNCTGT TCTTGGCCCT GAGCCGGGGC   541 AGGAACTGCN NNNNNCANAT ATCCTGTTTG GNCCNNATTC NNCTGTTCCA TCTGTTCCTG   601 ANNNTGNNNN NNNNTTCTCT ATTCTCAGNN NNNNATTTTT CCATGCCTTG NAAAATGGCG   661 TTACNTTNNN CTAGCTTGCN NNNNNNACAG GTGGGGGTCT TTCNTTCCCC CCTTTTTCTG   721 GANACTAANT AAANNCNTNT ATTTTATCGN CNNNNNNNAA NTANTACATA ATGNANTTNC 781 CTNANNNCNA ANNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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