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pMXs-3xFLAG-KLF4(L507N)

Retroviral expression vector of human KLF4, mutant L507N.

Catalog number RDB19025
Resource name pMXs-3xFLAG-KLF4(L507N)
Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (L507N) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
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External Database
human KLF4

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
          付加的提供条件:
          pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19025 pMXs-3xFLAG-KLF4(L507N) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMXs-3xFLAG-KLF4(L507N) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19025).

          Reference section:

          Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19025_B1Ecp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F (Pr0352)
          Region: pol region,insert 5'(3xFLAG,hKLF4(L507N) 5')
          Sequence file: RDB19025_B1Eca.seq check
          >D07716C4_B1Ec_2_MMLV-pol_F_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGNATCGNN GCTTGGNNAC NCGCCGCCCN CGTGAAGGCT
             61 GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCGGATC TAGCTAGTTA ATTAAGGATC
            121 CCAGTGTGGT GGTACGGGAA TTCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
            181 ATGACATCGA CTACAAGGAT GACGATGACA AGGCTGTCAG CGACGCGCTG CTCCCATCTT
            241 TCTCCACGTT CGCGTCTGGC CCGGCGGGAA GGGAGAAGAC ACTGCGTCAA GCAGGTGCCC
            301 CGAATAACCG CTGGCGGGAG GAGCTCTCCC ACATGAAGCG ACTTCCCCCA GTGCTTCCCG
            361 GCCGCCCCTA TGACCTGGCG GCGGCGACCG TGGCCACAGA CCTGGAGAGC GGCGGAGCCG
            421 GTGCGGCTTG CGGCGGTAGC AACCTGGCGC CCCTACCTCG GAGAGAGACC GAGGAGTTCA
            481 ACGATCTCCT GGACCTGGAC TTTATTCTCT CCAATTCGCT GACCCATCCT CCGGAGTCAG
            541 TGGCCGCCAC CGTGTCCTCG TCAGCGTCAG CCTCCTCTTC GTCGTCGCCG TCGAGCAGCG
            601 GCCCTGCCAG CGCGCCCTCC ACCTGCAGCT TCACCTATCC GATCCGGGCC GGGAACGACC
            661 CGGGCGTGGC GCCGGGCGGC ACGGGCGGAG GCCTCCTCTA TGGCAGGGAG TCCGCTCCCC
            721 CTCCGACGGC TCCCTTCNAC CTGGCGGACN TCAACGACGT GAGCCCCTCG GGCGGCTTCG
            781 TGGCCGANCT CCTGCGGCCA GAATTGGACC CGGTGTACAT TCCGCCGCAN CAGCCGCAGC
            841 CGCCAGGNGG CGGGCTGATG GNNAAGTTCG TGCTGAAGGC GTCNCTGANC GCCCCTGGCA
            901 NCNAGTACNG NAGCCCGNNN NTCNTCANCG TCNGCAAAGG CAGCCNNNNN NGCAGCCACC
            961 CGGNGGNGGT GGCGCCCTAC NNCGGCGGGN CNNCGCNNAC NNNCCNNNNA TNNAGCNNNA
          1021 NGNGNNNNCN TNNNNNNNCC NNNNGGGNNN NNNNNNN
          //
          Primer: pMXs-3LTRup_R2 (Pr0365)
          Region: insert 3'(attB2,hKLF4(L507N) 3')
          Sequence file: RDB19025_B1Ecb.seq check
          >D07716C4_B1Ec_2_pMXs-3LTRup_R2_F09_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTN ANCGTCGANT NNNNNNGTAC ATAATGGATT TCCTTACGCG
             61 AAATACGGGC ANNCATGGCC TGCCCGGTTA TTATTATTTT TGACACCAGA CCAACTGGTA
            121 ATGGTAGCGA CCGGCGCTCA GCTGGAATAT CACCACTTTG TACAAGAAAG CTGGGTCGGC
            181 GCGCCCACCC TTTTAAAAAT GTCTCTTCAT GTGGTTGGCG AGGTGGTCCG ACCTGGAAAA
            241 TGCTCGGTCG CATTTTTGGC ACTGGAACGG GCGGTGCCCC GTGTGTTTAC GGTAGTGCCT
            301 GGTCAGTTCA TCTGAGCGGG CGAATTTCCA TCCACAGCCG TCCCAGTCAC AGTGGTAAGG
            361 TTTCTCACCT GTGTGGGTTC GCAGGTGTGC CTTGAGATGG GAACTCTTTG TGTAGGTTTT
            421 GCCGCAGCCC GCGTAATCAC AAGTGTGGGT GGCGGTCCTT TTCCGGGGCC ACGATCGTCT
            481 TCCCCTCTTT GGCTTGGGCT CCTCTGGCAT GCAGGAACCG GGTGGCATGA GCTCTTGGTA
            541 ATGGAGCGGC GGGACTTGCG GCTGCATCTG ATCGGGCAGG AAGGATGGGT AATTGGGCCC
            601 CGGGTGGGGA TGGAAGCCGG GAGGAAGCGG CAGGGCAGGG TGACAGTCCC TGCTGCTCAG
            661 CACTTCCTCA AGACCCAGGG TCGGGGTAGT CCTGCTGGGG AGCTGCCGCC CCAGGGGGAA
            721 GTCGTGTGCA GCCGGCCGGT GGCCATTGCT GANAGGGGGT CCAGCGCCCA AGTGGGTGCA
            781 CGAAGAGACC GCCTCCTGCT TGNTCTTGGG GCACGTGCGC GGCGGCCCGC CGTTGTNNGN
            841 CGCCACCANC ACCGGGNGGC TGCCGTCNNG NCTGCCTTTG CTGACGCTGA TGANCNANGG
            901 GNTGCCNNAN TCGCTGNCAG GGNNNNNNAN NNNCNNNTNC NNNACGAANN NGNCNNNNNN
          961 NCNNNNNNNN NN
          //
          Primer: gag-N_R (Pr0745)
          Region: MMLV psi,5' LTR
          Sequence file: RDB19025_B1Ecc.seq check
          >D07716C4_B1Ec_2_gag-N_R_G09_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NNTNNTTTTN NGANAAATAC NGAAACNCAG TCAGACAGAG
             61 ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG CGGCTTCGGT CCCAAACCGA
            121 AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC TCGTCTCCTA CCAGAACCAC
            181 ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA CGATCGGGAT TTTTGGACTC
            241 AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG TCTCCCAGGG TTGCGGCCGG
            301 GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT ACAGAGCTAG TTAGCTAACT
            361 AGTACCGACG CAGGCGCATA AAATCAGTCA TAGACACTAG ACAATCGGAC AGACACAGAT
            421 AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG TCCCTGGGCA GGGGTCTCCC
            481 GATCCCGGAC GAGCCCCCAA ATGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG
            541 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG
            601 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCGCCGAG TGAGGGGTTG
            661 TGGGCTCTTT TATTGAGCTC GGGGANCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG
            721 ATTGGTTAGT TCAAATAANG CACANGGTCA TTTCANGTCC TTNNGGCACC CTGNAAACAT
            781 CTGATGGTTC TCTAGAAACT GCTGNNGGCT GGNNCGCATC TGGGGNNCAT CTGTNCTTGG
            841 CCNNNANCCG GGGCAGGANN NGCTNANCAC ANATATCCNG TTNNGNCNAT ANTCAGCTNN
          901 NCCATNNGNT CNNNNNNNNN N
          //
          Primer: lacIpro-B_F (Pr0724)
          Region: 3' LTR
          Sequence file: RDB19025_B1Ecd.seq check
          >D07716C4_B1Ec_2_lacIpro-B_F_H09_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN NCNNNTCNGT GNNCGNNGAA NCGGAANNNC GCCCAATACG
             61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA
            121 AATACTTAAG AAAAAAAACC AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC
            181 TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG
            241 ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG
            301 GACTGGCGCC CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC
            361 GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA
            421 GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC
            481 GCATCTGGGG ACCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA
            541 TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC
            601 TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG
            661 ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC CTTGCAAAAT GGCGTTACTT
            721 AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC CCCCTTTTTC TGGAGACTAA
            781 ATAAAATCTT TTATTTTATC GTCGACTTTA AATAGTACAT AATGGANTTC CTTACGCGAA
            841 ATACNGGCAG ACATGGNCTG NCCNGNNATT ATTANTTTTG ACACNANNNC NNCNGGNAAT
            901 GGNANCGANN GGNGCTCANC NNGNNNTATC ANCNNCNTNN NNNNNNAANC NNGNNNNNNN
          961 NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

          2023.08.29

          GNP_filter3_RDBDEP_html_230702.pl