Strain Data Sheet

RBRC05477

Strain Information

Image
BRC No.RBRC05477
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(NDE-hsp-Cluap1,lacZ)41Hmd
Former Common nameNDE-hsp Qilin-lacZ (41), B6-NDE-Qilin/lacZ Tg#41
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. 0.7 kb segment containing Nodal NDE enhancer and Qilin cDNA-IRES LacZ genes were injected into the pronuclei of ((C57BL/6 x BALB/c)F1 x B6129-Qilin<tm1.1Hmd>)F1 fertilized eggs (2 copies). C57BL/6 and 129 mixed background.
Strain descriptionThis transgenic mouse expresses Cluap1 (Qilin) and lacZ genes in crown cells of embryonic node. Tg expression can be monitored by X-gal staining.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Cluap1 localizes preferentially to the base and tip of cilia and is required for ciliogenesis in the mouse embryo.
Botilde Y, Yoshiba S, Shinohara K, Hasegawa T, Nishimura H, Shiratori H, Hamada H
Dev. Biol., 381(1), 203-212 (2013). 23742838

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cluap1 clusterin associated protein 1UNCluap1 mouse Nodal NDE-enhancer, mouse Hsp68 promoter
lacZ beta-galactosidase (E. coli)UN
loxP phage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Nodal NDE-enhancer, Mouse Hsp68 promoter, Mouse Qilin gene cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ gene, phage P1 loxP sequence
    Research applicationCre/loxP system
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 381(1), 203-212 (2013).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data