Strain Data Sheet

RBRC05705

Strain Information

Image
BRC No.RBRC05705
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Opn5<tm1.1>
Former Common nameOpn5 KO, B6-Opn5 KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Takashi Yoshimura, Graduate School of Bioagricultural Science, Nagoya University in 2009. Bruce 4 ES cells were used. Neo cassette was removed by crossing with CAG-Cre mice.
Strain descriptionOpn5 knock-out mice. EGFP was inserted into the exon 1 of Opn5.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Impaired Circadian Photoentrainment in Opn5-Null Mice.
Ota W, Nakane Y, Hattar S, Yoshimura T
iScience, 6:299-305 (2018). 30240620

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)17EGFP
Opn5
MGI:2662912		opsin 517Opn5<tm1.1> targeted mutation 1.1, Takashi Yoshimura
loxP phage P1 loxP17loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circardian behavior entrainment(MP:0020476)
    • Detailed phenotype data

    Ordering Information

    Donor DNAjellyfish EGFP cDNA, Simian virus 40 (SV40) polyA, Phage P1 loxP site, mouse Opn5 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Sensorineural Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. iScience, 6:299-305 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.
    DepositorTakashi Yoshimura (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data