Strain Data Sheet

RBRC09245

Strain Information

Image
BRC No.RBRC09245
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ehmt2<tm2Yshk>
Former Common nameG9a-flox, B6-Ehmt2 floxed
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Makoto Tachibana and Yoichi Shinkai, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used to generate the mutant mice. C57BL/6 mixed background.
Strain descriptionG9a (Ehmt2) gene floxed mice. Exon 26, an exon 27/28 (cDNA) and a PGK-neo cassette were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6]
References
Functional dynamics of H3K9 methylation during meiotic prophase progression.
Tachibana M, Nozaki M, Takeda N, Shinkai Y
EMBO J., 26, 3346-3359 (2007). 17599069

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ehmt2
MGI:2148922		euchromatic histone lysine N-methyltransferase 217Ehmt2<tm2Yshk>
MGI:3718575		targeted mutation 2, Yoichi Shinkai
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal female meiosis(MP:0005168)

  • abnormal fertility/fecundity(MP:0002161)

  • abnormal gametogenesis(MP:0001929)

  • arrest of male meiosis(MP:0008261)

  • azoospermia(MP:0005159)
    • more 6 phenotypes
  • decreased litter size(MP:0001935)

  • decreased male germ cell number(MP:0004901)

  • decreased oocyte number(MP:0005431)

  • female infertility(MP:0001926)

  • male infertility(MP:0001925)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Ehmt2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J, 26, 3346-3359 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYoichi Shinkai (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data