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pMY (#RDB03032)

Complete genome of JC virus, archetype

Clone info. Complete genome of JC virus, archetype.
Comment This clone contains whole genome of AB038250.1 (4,299nt to 5,121nt and 1nt to 4,298nt) in BamHI site of pUC19 by quality test.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genome Genomic DNA
Depositor|Developer Yogo, Yoshiaki |
 
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03032 pMY DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMY was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03032).

Reference section:

Yogo, Y., Kitamura, T., Sugimoto, C., Ueki, T., Aso, Y., Hara, K., Taguchi, F., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17355_A9F3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536)
Region: CAP binding site,lac pro,lacO,insert 5'
Sequence file: RDB17355_A9F3g.seq check
>03032_17355_A9F3_2_pUC-CAPbs_up_F_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNC NNCGCNNNAA TGTGAGTTAG CTCACTCATT AGGCACCCCA
   61 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT
  121 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC
  181 TAGAGGATCC TGTGTTTTCA TCATCACTGG CAAACATTTC TTCATGGCAA AACAGGTCTT
  241 CATCCCACTT CTCATTAAAT GTATTCCACC AGGATTCCCA TTCATCTGTT CCATAGGTTG
  301 GCACCTAAAA ACAAAAAATT AAGTTTATTG TAAAAAACAA AATGCCCTGC AAAAGAAAAA
  361 TTGTGGTTTA CCTTAAAGCT TTAGATCCCT GTAGGGGGTG TCTCCAAGAA CCTTCTCCCA
  421 GCAATGAAGA GCTTCTTGGG TTAAGTCACA CCCAAACCAT TGTCTGAAGC AATCAAAGCA
  481 ATAGCAATCT ATCCACACAA GTGGGCTGCT TCTTAAAAAT TTTCTGTTTC TATGCCTTAA
  541 TTTTAGCATG CACATTAAAC AGGGGCAATG CACTGAAGGA TTAGTGGCAC AATTAGGCCA
  601 TTCCTTGCAA TAAAGGGTAT CAGAATTAGG AGGAAAATCA CAACCAACCT CTGAACTATT
  661 CCATGTACCA AAATCAGGCT GATGAGCAAC TTTTACACCT TGTTCCATTT TTTTATATAA
  721 AAAATTCATT CTCTTCATTT TGTCTTCGTC CCCACCTTTA TCAGGNTGAA GTTCTTTGCA
  781 TTTTTTCAGA TAAGCTTTTC TCATGACAGG AATGTTTCNN CATGCAGATC TATCAANGCC
  841 TAATAAATCC NTGANCTCCA NGGATTCCTC CCNATTCAGC ACTTTNGTCC ATTTTTTGCT
  901 TTTTGTAGCA AAAAATTANN GCAAAAANNN NAAAAANNAN NNNNNNTTCC NNGGCCNCCN
961 AAAAAAGCNN NNNNNCCCNN ANNANTTNNG ANTAANNTNN GNNNGNNNNN NNNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: insert 3'
Sequence file: RDB17355_A9F3h.seq check
>03032_17355_B1HpF_pUC-M13_-100_H02_23_ABI24.ab1
    1 NNNNNNNNNN NANNNNGGNA NGCCNGGGNT TTCCCNGTCA CGACGTTGTA AAACGACGGC
   61 CAGTGAATTC GAGCTCGGTA CCCGGGGATC CCAACACTCT ACCCCACCTA AGAAGAAAAA
  121 AAAGGTAGAA GACCCTAAAG ACTTTCCTGT AGATCTGCAT GCATTCCTCA GTCAAGCTGT
  181 GTTTAGTAAT AGAACTGTTG CTTCTTTTGC TGTGTATACC ACTAAAGAAA AAGCTCAAAT
  241 TTTATATAAG AAACTTATGG AAAAATATTC TGTAACCTTT ATAAGTAGAC ATGGTTTTGG
  301 GGGTCATAAT ATCTTGTTTT TTCTAACACC ACATAGACAT AGAGTTTCAG CAATTAATAA
  361 TTACTGTCAA AAACTATGTA CCTTTAGTTT TTTAATTTGT AAAGGTGTGA ATAAGGAATA
  421 CTTGTTTTAC AGTGCCTTGT GTAGACAGCC ATATGCAGTA GTGGAAGAAA GTATTCAGGG
  481 GGGCCTTAAG GAGCATGACT TTAACCCAGA AGAACCAGAA GAAACTAAGC AGGTTTCATG
  541 GAAATTAGTT ACACAGTATG CTTTGGACAC CAAGTGTGAG GATGTTTTCT TGCTTATGGG
  601 CATGTACTTA GACTTTCAGG AAAACCCTTT GCNATGCAAA AAATGNGAAA AAAANGNNCA
  661 GCCAAATCAC NTNNACCATC ATGAAAAGCA CTATTATNNN GNCNNANTTT TNNNNNANNN
721 NNAAANNNNA AAAANNNNNT NNNNNNNNNN
//
Primer: JCsp_f1 (Pr0628)
Region: insert mid
Sequence file: RDB17355_A9F3i.seq check
>03032_17355_A9F3_2_JCsp_f1_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGN CCCNNCAAAA AGAANAGGAG AAAGGAAGGA NCCCGTGCAA
   61 GTTCCAAAAC TTCTTATAAG AGGAGGAGTA GAAGTTCTAG AAGTTAAAAC TGGGGTTGAC
  121 TCAATTACAG AGGTAGAATG CTTTTTAACT CCAGAAATGG GTGACCCAGA TGAGCATCTT
  181 AGGGGTTTTA GTAAGTCAAT TTCTATATCA GATACATTTG AAAGTGACTC CCCAAATAAG
  241 GACATGCTTC CTTGTTACAG TGTGGCCAGA ATTCCACTAC CCAATCTAAA TGAGGATCTA
  301 ACCTGTGGAA ATATACTAAT GTGGGAGGCT GTGACCTTAA AAACTGAGGT TATAGGGGTG
  361 ACAACTTTGA TGAATGTGCA CTCTAATGGT CAAGCAACTC ATGACAATGG TGCAGGAAAG
  421 CCAGTGCAGG GCACCAGCTT TCATTTTTTT TCTGTTGGGG GGGAGGCTTT AGAATTACAG
  481 GGGGTGGTTT TTAATTACAG AACAAAGTAC CCAGATGGAA CAATTTTTCC AAAGAATGCA
  541 ACAGTGCAAT CTCAAGTAAT GAACACAGAG CACAAGGCGT ACCTAGATAA GAACAAAGCA
  601 TATCCTGTTG AATGTTGGGT TCCTGATCCC ACCAGAAATG AAAACACAAG ATATTTTGGG
  661 ACACTAACAG GAGGAGAAAA TGTTCCTCCA GTTCTTCATA TAACAAATAC TGCNACAACA
  721 GTGCTGCTTG ATGAATTTGG TGTTGGGCCA CTTTGCNAAG GTGACAACTT GTATTTGTCA
  781 GCTGTTGATG TTTGTGGAAT GTTTACTAAC AGATCTGGTT CCCNNCANTG GAGAGGANTG
  841 TCCNGATATT TNNNNGTTCA GCTCANAAAA AGGANGGTTA AAAACCCCTA CCCNATTTCT
  901 TNCCTTCTTA CTGATTTGAN TNNNANAANN NNCCCTNNAN TTGANGGGNA NCCTANNNAN
961 GGNANNNNNG CNNNNNNNNN N
//
Primer: JCsp_R2 (Pr0635)
Region: insert mid
Sequence file: RDB17355_A9F3j.seq check
>03032_17355_A9F3_2_JCsp_R2_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNNCTTTAT TAAAATGTAC TGCATATTAC AATAAAGGCT
   61 TTTGGTTACA GCATCTTTGT TTGCAACTGT CCATATCTGT CAACATATCT CATCATGTCT
  121 GGGTCCCCTG GAAGTTCCTC TGTCCCCTCA AAAACTCTAA CCTCCTCTAC CTGAGCATCC
  181 ATACCATACA TAGGCTGCCC ATCAACTCTA GGGGTCCTTC TGTTAATCAA ATCAGTAAGA
  241 AGGAAAGAAA TTGGGTAGGG GTTTTTAACC CTCCTTTTTC TGAGCTGAAC CTTAAAATAT
  301 CTGGACAGTC CTCTCCACTG CTGGGAACCA GATCTGTTAG TAAACATTCC ACAAACATCA
  361 ACAGCTGACA AATACAAGTT GTCACCTTTG CAAAGTGGCC CAACACCAAA TTCATCAAGC
  421 AGCACTGTTG TGGCAGTATT TGTTATATGA AGAACTGGAG GAACATTTTC TCCTCCTGTT
  481 AGTGTCCCAA AATATCTTGT GTTTTCATTT CTGGTGGGAT CAGGAACCCA ACATTCAACA
  541 GGATATGCTT TGTTCTTATC TAGGTACGCC TTGTGCTCTG TGTTCATTAC TTGAGATTGC
  601 ACTGTTGCAT TCTTTGGAAA AATTGTTCCA TCTGGGTACT TTGTTCTGTA ATTAAAAACC
  661 ACCCCCTGTA ATTCTAAAGC CTCCCCCCCA ACAGAAAAAA AATGAAAGCT GGTGCCCTGC
  721 ACTGGCTTTC CTGCACCATT GTCATGAGTT GCTTGACCAT TAGAGTGCAC ATTCATCAAA
  781 GTTGTCACCC CTATAACCTC AGTTTTTAAG GTCACAGCCT CCCACATTAG TATATTTCCA
  841 CANGTTAGAT CCTCATTTAG ATTGGGTAGT NNAATTCTGG NCACACTGTA ACNAGGAANC
  901 ATGTCCTTAT TTGGGGANTC ANTTTCAAAT GTATCTGATA TAGAAATTGA CTTACTAAAA
961 CCCCNNANAT GCTCATCNNG GNNACCCATT TNNNGNNNNT NNAAANNNNN NNNNN
//
Primer: Jcsp_f4 (Pr0627)
Region: insert mid
Sequence file: RDB17355_A9F3k.seq check
>03032_17355_A9F3_2_JCsp_f4_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NTTNNGNACT TTGTCNNTTT TTGCTTTTTG TAGCAAAAAA
   61 TTAGTGCAAA AAAGGGAAAA ACAAGGGAAT TTCCCTGGCC TCCTAAAAAG CCTCCACGCC
  121 CTTACTACTT CTGAGTAAGC TTGGAGGCGG AGGCGGCCTC GGCCTCCTGT ATATATAAAA
  181 AAAAGGGAAG GTAGGGAGGA GCTGGCTAAA ACTGGATGGC TGCCAGCCAA GCATGAGCTC
  241 ATACCTAGGG AGCCAACCAG CAGACAGCCA GAGGGAGCCC TGGCTGCATG CCACTGGCAG
  301 TTATAGTGAA ACCACTCCCA TAGTCCTTAA TCACAAGTAA ACAAAGCACA AGGGGAAGTG
  361 GAAAGCAGCC AAGGGAACAT GTTTTGCGAG CCAGAGCTGT TTTGGCTTGT CACCAGCTGG
  421 CCATGGTTCT TCGCCAGCTG TCACGTAAGG CTTCTGTGAA AGTTAGTAAA ACCTGGAGTG
  481 GAACTAAAAA AAGAGCTCAA AGGATTTTAA TTTTTTTGTT AGAATTTTTG CTGGATTTTT
  541 GCACAGGTGA AGACAGTGTA GACGGGAAAA AAAGGCAGAA ACACAGTGGT TTGACTCAGC
  601 AGACATACAG TGCTTTGCCT GAACCAAAAG CTACATAGGT AAGTAATGTT TTTTTTTGTG
  661 TTTTCAGGTT CATGGGTGCC GCACTTGCAC TTTTGGGGGA NCTAGTTGCT ACTGTTTCTG
  721 ANGCTGCTGC TGCCACAGGA TTTTCAGTAG CTGAAATTGC TGCTGGAGAN GCTGCTGCTA
  781 CTATAGAAGT TGAAATTGCA TCCCTTGCTA CTGTAGANGG GNNTACAAGT ACCTCTGANG
  841 CTATANCTGC AATAGGCCTT ACTCCTGNNA CATATGCTGT AANTACNNGA GCNNCNGGGG
  901 GCTGTANCNN GGNTTTNNTG NNNTNGGTTC AAACTNNNAN NNGGTGGNNG NGCTNTNGCT
  961 CANTTGGGNN NTNNANTTTT TGCTGNNNNG GNATCATAAA GTTTNNNNNN TNNGGNNTTT
1021 TNANCANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yogo, Y., Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. J. Virol., 64, 3139-3143 (1990). PMID 2159570. [link to RRC of NBRP]