RDB03379 - Human HLA-C*15:02:01 cDNA

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Human HLA-C*15:02:01 cDNA

Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number	RDB03379
Resource name	Human HLA-C*15:02:01 cDNA
Alternative name	HLA-Cw*15021
Clone info.	Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human HLA-Cw*15021 cDNA
Depositor\|Developer	Akatsuka, Yoshiki \|

Other clones in our bank

human HLA-Cw*15021

External Database
Human HLA-Cw*15021

Reference sequence

Sequence (full)	RDB03379zzt02.seq compiled by us
Remarks, protocol and/or map (gif)	RDB03379.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)

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提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB03379	Human HLA-C*15:02:01 cDNA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Human HLA-C*15:02:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03379).

Reference section:

Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19812_B2L6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB19812_B2L6a.seq
>03379_19812_B2L6_3_CMV_Forward_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNNNNNNNN 61 NTNNNNNNGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT 121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTTTCTCC 181 CCAGACGCCG AGATGCGGGT CATGGCGCCC CGAACCCTCC TCCTGCTGCT CTCGGGAGCC 241 CTGGCCCTGA CCGAGACCTG GGCCTGCTCC CACTCCATGA GGTATTTCTA CACCGCTGTG 301 TCCCGGCCCG GCCGCGGAGA GCCCCACTTC ATCGCAGTGG GCTACGTGGA CGACACGCAG 361 TTCGTGCGGT TCGACAGCGA CGCCGCGAGT CCAAGAGGGG AGCCGCGGGC GCCGTGGGTG 421 GAGCAGGAGG GGCCGGAGTA TTGGGACCGG GAGACACAGA ACTACAAGCG CCAGGCACAG 481 ACTGACCGAG TGAACCTGCG GAAACTGCGC GGCTACTACA ACCAGAGCGA GGCCGGGTCT 541 CACATCATCC AGAGGATGTA TGGCTGCGAC CTGGGGCCCG ACGGGCGCCT CCTCCGCGGG 601 CATGACCAGT TAGCCTACGA CGGCAAGGAT TACATCGCCC TGAACGAGGA CCTGCGCTCC 661 TGGACCGCCG CGGACACGGC GGCTCAGATC ACCCAGCGCA AGTGGGANGC GGCCCGTGAG 721 GCGGAGCAGC TGANAGCCTA CCTGGANGGC ACGTGCGTGG AGTGGCTCCG CAGATACCTG 781 GAGAACGGGA ANGANACGCT GCAGCGCGCG GAACACCCAA AGACACACGT GACCCACCAT 841 CCCGTCTCTG ANCATGAGGC CACNCTGANG NGCTNGGCCC NGGGCTTCTA NCCCTGCGGA 901 NNTCACACTG ACCNNGNAGC NGGNNNGCGA GGACCAAACT CNNNANNCCN NNNTTGNGGA 961 NNNCANNNCC NGCNNNANNT GGNANNNNTN CNNNANNNNG NNNNNNNNNN //
Primer: BGH_rev3 (Pr0885) Region: bGH pA,insert 3' Sequence file: RDB19812_B2L6b.seq
>03379_19812_B2L6_3_bGH_rev3_G05_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGGG NGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC GTCTCAGGCT 121 TTACAAGCGA TGAGAGACTC ATCAGAGCCC TGGGCACTGT TGCTGGACGC AGCCTGAGAG 181 CAGCTCCCTC CTTTTCCACC TGAGCTCTTC CTCCTACACA TCACAACAGC CATCACAGCT 241 CCTAGGACAG CTAGGACAGC CAGGACAGCC AGGCCAGCAA CGATGCCCAC GATGGGGATG 301 GTGGGCTGGG AAGATGGCTC CCATCTCAGG GTGAGGGGCT CCGGCAGCCC CTCGTGCTGC 361 ACATGGCACG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGC CCACTTCTGG 421 AAGGTTCCAT CTCCTGCTGG CCTGGTCTCC ACAAGCTCGG TGTCCTGAGT TTGGTCCTCG 481 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AGCCCAGGGC CCAGCACCTC 541 AGGGTGGCCT CATGGTCAGA GACGGGATGG TGGGTCACGT GTGTCTTTGG GTGTTCCGCG 601 CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TATCTGCGGA GCCACTCCAC GCACGTGCCC 661 TCCAGGTAGG CTCTCAGCTG CTCCGCCTCA CGGGCCGCCT CCCACTTGCG CTGGGTGATC 721 TGAGCCGCCG TGTCCGCGGC GGTCCAGGAN CGCANGTCCT CGTTCANGGC GATGTAATCC 781 TTGCCGTCGT ANGCTAACTG GTCATGCCCG CGGANGANGC GCCCGTCNGG CCCCANGTCG 841 CANCCATACA TCCTCNGGAT GANGTGAGAN CCGGCCTCGC TCTGGTTGTA GTAGCCNCGC 901 ANTTTNNNCN NNTTCACTCN GNNANNCTGN NNNNNGNGCT NGTANTTCNN NNNNNNCNNN 961 NNCNNNNNNN NCNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB19812_B2L6c.seq
>03379_19812_B2L6_3_SV40pro_ori_F_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNCN CCTCTGCCTC TGNNCTATTC NNGAAGTAGT GAGGAGGCTT 61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 721 TGCCTGCTTG CCGAATATCA TGGTGGAANA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 781 CCGGCTGGGT GTGGCGGANC GCTATCANGA CATANCGTTG GCTACCCGNG ATATTGCTGA 841 AGAGCTTGGC GGCGAATGGG CTGANCGCTT CCNCGNGCTT TACNGNATCG CCGCNCCCNA 901 TTCNCANCNN ATCNCCTTCT ATCGCNNTCN NNNNNANTNC NTNCTGANCN GNNNNNNNGG 961 NNNTNNNNAN NNNNCNNNNC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.
user_report	Ichise, H., NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. Stem Cell Reports 9 (3): 853-867 (2017). PMID 28867344.

2023.05.02

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