Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-HFF81

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14483_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14483_A6L7a.seq
>06181_14483_A6L7_nmt1Fw_A01_01_ABI08.ab1     1 AGGAAAATTA TTAATCAGGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA    61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC   121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT   181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT   241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT   301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA   361 GGACGACGAT GACAAAGATA TCATGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC   421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT   481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG   541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA   601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT AAGATGATTC   661 TTTTATCGTC GATAACGATG AGGATGTAGA CGATTATGAT GAGAGTGATT AGCTTCTAAA   721 ATTTGGTGTT TTCAAACGCT ATTTACAGTA CAATCTGAAA AAATTGGATG AACATTGTAA   781 ATGGTAGGAT ACGAGTTCCA ATTAATGTAT TTTTTTAAGT ATTACACTTA GCTTATTAAT   841 TTTATATATA CATTCTACCT CTGTGGGTTT AAAGACAACT CGTACACATA CTCAAATTAT   901 TTATGAACAT TATAATGCAT ATTCGTACTG TCTATTAAAC ATTTTTTTTT GACATTTAAA   961 AATTTCGTTT ACTAACGTAG AAAGCGCTGA TAGTGAGCTA CTATTTTATA TCCTAGGAAC  1021 AAGAATTCAT AATAGTTAAG TAAGCTCGAG CAATACTTGA TTATTTCGCT AAAAAGCGCA 1081 ATTTTCGAAC AAGTCTC //
Primer: LEU1pro_F Sequence file: RDB14483_A6L7b.seq
>06181_14483_A6L7_LEU1pro_F_B01_02_ABI08.ab1     1 AATAGAGTGC CAACAGTGTT GAGTTTCCCG AAACCAGGCA TTTTTGCGGT AGGGTCTGAA    61 ACTTTCACGA AACCATTTCA CAACAAAACT TCTTCAACAA CATGTGTGCA AAGAAAATCG   121 TCGTCTTACC AGGAGACCAT ATTGGCCCTG AAATTGTTGC TTCTGCCTTG GAGGTTTTGA   181 AAGTCGTTGA GAAGAAGCGA CCTGAGTTAA AACTCGAGTT TGAAGAACAC AAGATTGGAG   241 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG   301 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC   361 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC   421 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG   481 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA   541 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT   601 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACACCAAT GTTTATAACC AAGTTTTATC   661 TTGTTTGTCT ACATGGTATT TTACATTCAT CTACATACAT CTTTCATTGG CTTTGTACAT   721 AGTTATCATT ACAAGTCTAA AAAAAATTCA CTCTTTTCTT ATTCAATGTC AATCCAAGAG   781 AAAAGATTGT GGTAATGTTG TAGGAGCATG TTTAATAAAA TTACTATAGC AAATTACTTT   841 TTTATTCCCA AAGTGTTTAT CTATAATAGT TAATATTTTA GTCGCTACAT AAAAATTTTA   901 CCAAAGAGTA CTTTGTATAC TAAATTCTAA AATGCCTTTC TGACATAAAA CGCCCTAGGA   961 AAAAACAAAA CCGCCAAACA AAGCCATCGA CTTTTTCCAA TAAGCGAGCT GAAAAAATTT  1021 TACATAAGTC TTTTTTTAAT GCCTGAGAAA GTCTTTGGCC TGGAATTGCT TCAATCAGCT 1081 CTCTATATCA CATGGCT //
Primer: ars_R1 Sequence file: RDB14483_A6L7c.seq
>06181_14483_A6L7_ars_R1_C01_03_ABI08.ab1     1 TGGATTCGGT TTTATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA    61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT   121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA   181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA   241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT   301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA   361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA   421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG   481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT   541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG   601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG   661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA   721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG   781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG   841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAAACTG   901 GTGGGCCTTA GGTAAAAAGC ATCGTTTTCT TATCTTTGAG ATCGCAAATT CGCAGACATT   961 GGGAAATACC GTCAAGCTAC AATATGCATC TGGTGTTGTA CCAAAAATTG CCTTCTTGGG  1021 CTCATATCAC AATTTGCCAA TTACAGTTGC AGGCGGAGGG TTATATACAG GCTTCAAGGA 1081 AGTGGATTTT ACCTTTTGGG GG //
Primer: f1ori_R3 Sequence file: RDB14483_A6L7d.seq
>06181_14483_A6L7_f1ori_R3_H04_22_ABI24.ab1     1 GCGAAGGGTC ATGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC    61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC   121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA   181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG   241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC   301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC   361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT   421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA   481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT   541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG   601 GGCCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA   661 TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA   721 CAAAATGGAT GATCTCTTCT CCATTTGTTT TCCCTTTCTT CTTGCAAAAC GCGTTTTACA   781 AAGAGATGAA CATGCTATTT TCTGATTAAT TTTTTTTCCT ATAGTTTTCT ATTTTTGTAC   841 GCTCCTTGGG GAAGTCCCAA ATGTAAAATC CGGGGGTTAT GGGTTATTTG CCGGACCCTT 901 TCCATTTATT GGTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl