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pDUAL-HFF81 (#RDB06181)

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364048.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06181z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06181 pDUAL-HFF81 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-HFF81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06181).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14483_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14483_A6L7a.seq check
>06181_14483_A6L7_nmt1Fw_A01_01_ABI08.ab1
    1 AGGAAAATTA TTAATCAGGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
  241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
  301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA
  361 GGACGACGAT GACAAAGATA TCATGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC
  421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT
  481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG
  541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA
  601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT AAGATGATTC
  661 TTTTATCGTC GATAACGATG AGGATGTAGA CGATTATGAT GAGAGTGATT AGCTTCTAAA
  721 ATTTGGTGTT TTCAAACGCT ATTTACAGTA CAATCTGAAA AAATTGGATG AACATTGTAA
  781 ATGGTAGGAT ACGAGTTCCA ATTAATGTAT TTTTTTAAGT ATTACACTTA GCTTATTAAT
  841 TTTATATATA CATTCTACCT CTGTGGGTTT AAAGACAACT CGTACACATA CTCAAATTAT
  901 TTATGAACAT TATAATGCAT ATTCGTACTG TCTATTAAAC ATTTTTTTTT GACATTTAAA
  961 AATTTCGTTT ACTAACGTAG AAAGCGCTGA TAGTGAGCTA CTATTTTATA TCCTAGGAAC
 1021 AAGAATTCAT AATAGTTAAG TAAGCTCGAG CAATACTTGA TTATTTCGCT AAAAAGCGCA
1081 ATTTTCGAAC AAGTCTC
//
Primer: LEU1pro_F
Sequence file: RDB14483_A6L7b.seq check
>06181_14483_A6L7_LEU1pro_F_B01_02_ABI08.ab1
    1 AATAGAGTGC CAACAGTGTT GAGTTTCCCG AAACCAGGCA TTTTTGCGGT AGGGTCTGAA
   61 ACTTTCACGA AACCATTTCA CAACAAAACT TCTTCAACAA CATGTGTGCA AAGAAAATCG
  121 TCGTCTTACC AGGAGACCAT ATTGGCCCTG AAATTGTTGC TTCTGCCTTG GAGGTTTTGA
  181 AAGTCGTTGA GAAGAAGCGA CCTGAGTTAA AACTCGAGTT TGAAGAACAC AAGATTGGAG
  241 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG
  301 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC
  361 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC
  421 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG
  481 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA
  541 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT
  601 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACACCAAT GTTTATAACC AAGTTTTATC
  661 TTGTTTGTCT ACATGGTATT TTACATTCAT CTACATACAT CTTTCATTGG CTTTGTACAT
  721 AGTTATCATT ACAAGTCTAA AAAAAATTCA CTCTTTTCTT ATTCAATGTC AATCCAAGAG
  781 AAAAGATTGT GGTAATGTTG TAGGAGCATG TTTAATAAAA TTACTATAGC AAATTACTTT
  841 TTTATTCCCA AAGTGTTTAT CTATAATAGT TAATATTTTA GTCGCTACAT AAAAATTTTA
  901 CCAAAGAGTA CTTTGTATAC TAAATTCTAA AATGCCTTTC TGACATAAAA CGCCCTAGGA
  961 AAAAACAAAA CCGCCAAACA AAGCCATCGA CTTTTTCCAA TAAGCGAGCT GAAAAAATTT
 1021 TACATAAGTC TTTTTTTAAT GCCTGAGAAA GTCTTTGGCC TGGAATTGCT TCAATCAGCT
1081 CTCTATATCA CATGGCT
//
Primer: ars_R1
Sequence file: RDB14483_A6L7c.seq check
>06181_14483_A6L7_ars_R1_C01_03_ABI08.ab1
    1 TGGATTCGGT TTTATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA
   61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT
  121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA
  181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA
  241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT
  301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA
  361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA
  421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG
  481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT
  541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG
  601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG
  661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA
  721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG
  781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG
  841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAAACTG
  901 GTGGGCCTTA GGTAAAAAGC ATCGTTTTCT TATCTTTGAG ATCGCAAATT CGCAGACATT
  961 GGGAAATACC GTCAAGCTAC AATATGCATC TGGTGTTGTA CCAAAAATTG CCTTCTTGGG
 1021 CTCATATCAC AATTTGCCAA TTACAGTTGC AGGCGGAGGG TTATATACAG GCTTCAAGGA
1081 AGTGGATTTT ACCTTTTGGG GG
//
Primer: f1ori_R3
Sequence file: RDB14483_A6L7d.seq check
>06181_14483_A6L7_f1ori_R3_H04_22_ABI24.ab1
    1 GCGAAGGGTC ATGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
  241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
  301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
  361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
  421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
  481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
  541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
  601 GGCCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA
  661 TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA
  721 CAAAATGGAT GATCTCTTCT CCATTTGTTT TCCCTTTCTT CTTGCAAAAC GCGTTTTACA
  781 AAGAGATGAA CATGCTATTT TCTGATTAAT TTTTTTTCCT ATAGTTTTCT ATTTTTGTAC
  841 GCTCCTTGGG GAAGTCCCAA ATGTAAAATC CGGGGGTTAT GGGTTATTTG CCGGACCCTT
901 TCCATTTATT GGTCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]
user_report Kanda, K., Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts. J. Biotechnol. 182-183: 68-73 (2014). PMID 24786825. [link to RRC of NBRP]