Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hPrP129M in pTA2 (#RDB06716)

Plasmid clone of human prion cDNA

Clone info. Plasmid clone of human prion cDNA. There is polymorphism at codon 129. This clone has Met at codon 129. Nucleotide sequence of 75 to 700 of AB300823.1 was cloned. Signal peptide and propeptide are not included.
Vector backbone pTA2 (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human hPrP 129M cDNA
Reference of insert sequence AB300823 (DDBJ accession number)
Depositor|Developer Nakajima, Osamu | Kikuchi, Yutaka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FEBS J., 275, 2965-2976 (2008))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FEBS J., 275, 2965-2976 (2008))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06716 hPrP129M in pTA2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hPrP129M in pTA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06716).

Reference section:

Kikuchi, Y., Kakeya, T., Nakajima, O., Sakai, A., Ikeda, K., Yamaguchi, N., Yamazaki, T., Tanamoto, K., Matsuda, H., Sawada, J., Takatori, K., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06716_A3Jh.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB06716_A3Jha.seq check
>06716_06716_A3Jh_3_T7_20bp_D02_11.ab1
    1 TACGTGGGAC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCCAATACTC
   61 GGAATTCCAT ATGAGCAAGA AGCGCCCGAA GCCTGGAGGA TGGAACACTG GGGGCAGCCG
  121 ATACCCGGGG CAGGGCAGCC CTGGAGGCAA CCGCTACCCA CCTCAGGGCG GTGGTGGCTG
  181 GGGGCAGCCT CATGGTGGTG GCTGGGGGCA GCCTCATGGT GGTGGCTGGG GGCAGCCCCA
  241 TGGTGGTGGC TGGGGACAGC CTCATGGTGG TGGCTGGGGT CAAGGAGGTG GCACCCACAG
  301 TCAGTGGAAC AAGCCGAGTA AGCCAAAAAC CAACATGAAG CACATGGCTG GTGCTGCAGC
  361 AGCTGGGGCA GTGGTGGGGG GCCTTGGCGG CTACATGCTG GGAAGTGCCA TGAGCAGGCC
  421 CATCATACAT TTCGGCAGTG ACTATGAGGA CCGTTACTAT CGTGAAAACA TGCACCGTTA
  481 CCCCAACCAA GTGTACTACA GGCCCATGGA TGAGTACAGC AACCAGAACA ACTTTGTGCA
  541 CGACTGCGTC AATATCACAA TCAAGCAGCA CACGGTCACC ACAACCACCA AGGGGGAGAA
  601 CTTCACCGAG ACCGACGTTA AGATGATGGA GCGCGTGGTT GAGCAGATGT GTATCACCCA
661 GTACGAGAGG GAATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]