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hPrP129V in pTA2 (#RDB06717)

Plasmid clone of human prion cDNA

Clone info. Plasmid clone of human prion cDNA. There is polymorphism at codon 129. This clone has Met to Val substitution at codon 129. Nucleotide sequence of 75 to 700 of AB300824.1 was cloned. Signal peptide and propeptide are not included.
Vector backbone pTA2 (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human hPrP 129V cDNA
Reference of insert sequence AB300824 (DDBJ accession number)
Depositor|Developer Nakajima, Osamu | Kikuchi, Yutaka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FEBS J., 275, 2965-2976 (2008))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FEBS J., 275, 2965-2976 (2008))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06717 hPrP129V in pTA2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hPrP129V in pTA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06717).

Reference section:

Kikuchi, Y., Kakeya, T., Nakajima, O., Sakai, A., Ikeda, K., Yamaguchi, N., Yamazaki, T., Tanamoto, K., Matsuda, H., Sawada, J., Takatori, K., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06717_A3Jh.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB06717_A3Jha.seq check
>06717_06717_A3Jh_3_T7_20bp_F02_17.ab1
    1 TGCATGGTCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CCAATACTCG
   61 GAATTCCATA TGAGCAAGAA GCGCCCGAAG CCTGGAGGAT GGAACACTGG GGGCAGCCGA
  121 TACCCGGGGC AGGGCAGCCC TGGAGGCAAC CGCTACCCAC CTCAGGGCGG TGGTGGCTGG
  181 GGGCAGCCTC ATGGTGGTGG CTGGGGGCAG CCTCATGGTG GTGGCTGGGG GCAGCCCCAT
  241 GGTGGTGGCT GGGGACAGCC TCATGGTGGT GGCTGGGGTC AAGGAGGTGG CACCCACAGT
  301 CAGTGGAACA AGCCGAGTAA GCCAAAAACC AACATGAAGC ACATGGCTGG TGCTGCAGCA
  361 GCTGGGGCAG TGGTGGGGGG CCTTGGCGGC TACGTGCTGG GAAGTGCCAT GAGCAGGCCC
  421 ATCATACATT TCGGCAGTGA CTATGAGGAC CGTTACTATC GTGAAAACAT GCACCGTTAC
  481 CCCAACCAAG TGTACTACAG GCCCATGGAT GAGTACAGCA ACCAGAACAA CTTTGTGCAC
  541 GACTGCGTCA ATATCACAAT CAAGCAGCAC ACGGTCACCA CAACCACCAA GGGGGAGAAC
  601 TTCACCGAGA CCGACGTTAA GATGATGGAG CGCGTGGTTG AGCAGATGTG TATCACCCAG
661 TACGAGAGGG AATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]