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hPrPSV129V in pTA2 (#RDB06719)

Plasmid clone of splice variant of prion

Clone info. Plasmid clone of splice variant of prion. This clone has Met to Val substitution at codon 129. This variant transcript was induced at low oxygen concentration in T98G cells.
Vector backbone pTA2 (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human hPrPSV 129V cDNA
Reference of insert sequence AB300826 (DDBJ accession number)
Depositor|Developer Nakajima, Osamu | Kikuchi, Yutaka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FEBS J., 275, 2965-2976 (2008))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FEBS J., 275, 2965-2976 (2008))。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB06719 hPrPSV129V in pTA2 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The hPrPSV129V in pTA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06719).

Reference section:

Kikuchi, Y., Kakeya, T., Nakajima, O., Sakai, A., Ikeda, K., Yamaguchi, N., Yamazaki, T., Tanamoto, K., Matsuda, H., Sawada, J., Takatori, K., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]