Expression vector of mouse VPS9-ankyrin-repeat protein (Varp) full-length (1-1048 aa).
Clone info. | Expression vector driven by the EF1 promoter for T7-tagged mouse VPS9-ankyrin-repeat protein (Varp) full-length (1-1048 aa), see Fig. 3A of Tamura, K. et al. Mol. Biol. Cell., 20 (12): 2900-2908, 2009. |
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Comment | Refer GenBank, accession AB455262 for mouse Varp sequence. Expression was confirmed by the depositor with immunoprecipitation and microscopic observation. |
Vector backbone | pEF-T7 (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | mouse Ankrd27 cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tamura. K. et al., Mol. Biol. Cell, 20 (12): 2900-2908, 2009); (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tamura. K. et al., Mol. Biol. Cell, 20 (12): 2900-2908, 2009); (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)。 MTAに書く付加的使用条件: pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB14334 | pEF-T7-Varp | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEF-T7-Varp was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14334). |
Reference section:
Marubashi, S., Ohbayashi, N., Fukuda, M., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885. [link to RRC of NBRP] |
Tamura, K., Ohbayashi, N., Maruta, Y., Kanno, E., Itoh, T., Fukuda, M., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885. [link to RRC of NBRP] |
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original | Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP] |
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