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pCMV-NICD 3xFLAG (#RDB14500)

Expression vector of mouse Notch1 active form N1deltaECD.

Alternative name pYY554-1, N1deltaECD
Clone info. Expression vector of mouse Notch1 active form. N1deltaECD in the Fig.4 of Sasaki, N., Development 138 (1); 55-64, 2011.
Comment Nucleotide sequence of 5372 to 7857nt of NM_008714.3 (1..9497nt) corresponding Ile of 1704aa to 2531aa in Fig.1 of Kato, H., FEBS Lett., 395, 221-224 (1996). Known difference: nucleotide substitution of G to A at 7159nt of NM_008714.3 (Glu at 2299aa to Lys).
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mouse Notch gene homolog 1 cDNA
Depositor|Developer Saga, Yumiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Sasaki, N., Development 138, 55-64, 2011) and an acknowledgment to the DEPOSITOR are requested.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sasaki, N., Development 138, 55-64, 2011)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14500 pCMV-NICD 3xFLAG DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-NICD 3xFLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14500).

Reference section:

Sasaki, N., Kiso, M., Kitagawa, M., Saga, Y., The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Development 138 (1): 55-64 (2011). PMID 21098559. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14500_A6Lcp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'
Sequence file: RDB14500_A6Lca.seq check
>D04132A1_A6Lc_1_CMV-Forward_A10_01_ABI08.ab1
    1 CTTTTATTTC CCCCGCCCGT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTAAGCTT GCGGCCGCGA ATTCCCACCA
  121 TGGATATTCC TTACAAGATT GAGGCCGTGA AGAGTGAGCC GGTGGAGCCT CCGCTGCCCT
  181 CGCAGCTGCA CCTCATGTAC GTGGCAGCGG CCGCCTTCGT GCTCCTGTTC TTTGTGGGCT
  241 GTGGGGTGCT GCTGTCCCGC AAGCGCCGGC GGCAGCATGG CCAGCTCTGG TTCCCTGAGG
  301 GTTTCAAAGT GTCAGAGGCC AGCAAGAAGA AGCGGAGAGA GCCCCTCGGC GAGGACTCAG
  361 TCGGCCTCAA GCCCCTGAAG AATGCCTCAG ATGGTGCTCT GATGGACGAC AATCAGAACG
  421 AGTGGGGAGA CGAAGACCTG GAGACCAAGA AGTTCCGGTT TGAGGAGCCA GTAGTTCTCC
  481 CTGACCTGAG TGATCAGACT GACCACAGGC AGTGGACCCA GCAGCACCTG GACGCTGCTG
  541 ACCTGCGCAT GTCTGCCATG GCCCCAACAC CGCCTCAGGG GGAGGTGGAT GCTGACTGCA
  601 TGGATGTCAA TGTTCGAGGA CCAGATGGCT TCACACCCCT CATGATTGCC TCCTGCAGTG
  661 GAGGGGGCCT TGAGACAGGC AACAGTGAAG AAGAAGAAGA TGCACCTGCT GTCATCTCTG
  721 ACTTCATCTA CCAGGGCGCC AGCTTGCACA ACCAGACAGA CCGCACCGGG GAGACCGCCT
  781 TGCACTTGGC TGCCCGATAC TCTCGTTCAG ATGCTGCAAA GCGCTTGCTG GAGGCCAGTG
  841 CAGATGCCAA CATCCAGGAC AACATGGGGC CGTACTCCGT TACATGCAGC AGTTTCTGCA
  901 GATGCTCAGG GTGTCTTCCA GATCCTGCTC CGGAACAGGG CCACAGATCT GGATGCCCGA
  961 ATGCATGATG GCACAACTCC ACTGATCCCT GGGCTGCGCG CCTGGGCGTG GGAGGGCCAT
 1021 GCTGGAAGGA CCTCATCAAC CTCACATGCT GACGTCCAAT GCCGTGATGG ACCTTAGGCA
1081 AGTCCGCTTG GCCATGGCCC GGACCGGCGG TGACCAAATG TGTGGGAT
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH p(A), 3xFLAG, insert 3'
Sequence file: RDB14500_A6Lcb.seq check
>D04132A1_A6Lc_1_hGHpolyA-R_B10_02_ABI08.ab1
    1 ACTAACCCCC CTCACCCCAA TAATATTATA GGAAGGACAC CTAGTCAGAC AAAATGATGC
   61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
  121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGGGATC ACTACTTGTC ATCGTCATCC
  181 TTGTAGTCGA TGTCATGATC TTTATAATCA CCGTCATGGT CTTTGTAGTC AGCCCGGGAT
  241 CCTTTAAATG CCTCTGGAAT GTGGGTGATC TGGGACGGCA TGGTGGTGGG CGGGCTGGAG
  301 ATGCCCTCGG ACCAATCAGA GATGTTGGAA TGCGGGGAGG AGCTGGACCA CTGGTCAGGG
  361 GACTCAGGGG ATGGGGTGAG GAAGGGGTGC TCTGGCACCT GCAGCTGGTG GCTGGGGGTG
  421 TTGTCCACAG GGGAGGAGGA GTAACTGTGC TGGGAAGGAG GGGTCAGGAA CTGGGTAGTG
  481 GTCATGGGTG GGACCATGGA GGATGGCAGT GATGTGGGCA GGGCCTGGCT TTCCTGGGGC
  541 AGAATGGTGT GCACAGGCAG ACTGCTGGGG CCCAGCGGTT GTACATCTGC CTGACTGGGC
  601 TCCCCACTCA AGAAGCTCCG GCCCAGGTGC CCATTGGCTG CCGAGCTCAC ACTGAGGTGT
  661 GGCTGTGATG GTGGCTGCAG GTTCTGAGGC TGGAGCTGTA AGTTCTGTGG CTGCACCTGC
  721 TGGGTCTGCA CCAGGTGAGG CTGTGTTGCC AGCCGTGTGT TGGGCAGGCC CTGGTAAATA
  781 ATCGGGGACA AGGTATTGGT GGAGAGGCTG CTGTGTAGTG GCCCCATCAT GCTATGCTGG
  841 AGGCCAGCTG CCTGTGTGCT CAGTGTGCCC GGCGTCACAC CCGGCCGTAG TGGGTTGTAC
  901 TGGGCTGGGC ACCATGCCAT TCTGGAGCCG GGAAAGCCAC TTACACTGGC CATTCAAGGC
  961 TTGCCGGTGC ACCCACGGTG GAATTTCATA GCCCCCGTGC CATTGGTACT TCAGCACTGT
 1021 GCTGGCACTG GGAAGGCTAC AGCAAGGTGG AGAGGCCGTT GGCGGGGATG GCTCAAAGCC
1081 AATAGGCTTA CCTCCAGTCA GTGCTGGCCA ATCTCAAGGC TGGCCTTGGG CCCACCAT
//
Primer: RDB14500#1 (Pr0429)
Region: insert mid
Sequence file: RDB14500_A6Lcc.seq check
>D04132A1_A6Lc_1_RDB14500#1_A04_01_ABI08.ab1
    1 CGGGCAGCTG CCTTGGGCTG CCGATACTCT CGTTCAGATG CTGCAAAGCG CTTGCTGGAG
   61 GCCAGTGCAG ATGCCAACAT CCAGGACAAC ATGGGCCGTA CTCCGTTACA TGCAGCAGTT
  121 TCTGCAGATG CTCAGGGTGT CTTCCAGATC CTGCTCCGGA ACAGGGCCAC AGATCTGGAT
  181 GCCCGAATGC ATGATGGCAC AACTCCACTG ATCCTGGCTG CGCGCCTGGC CGTGGAGGGC
  241 ATGCTGGAGG ACCTCATCAA CTCACATGCT GACGTCAATG CCGTGGATGA CCTAGGCAAG
  301 TCGGCTTTGC ATTGGGCGGC CGCGGTGAAC AATGTGGATG CTGCTGTTGT GCTCCTGAAG
  361 AACGGAGCCA ACAAGGACAT GCAGAACAAC AAGGAGGAGA CTCCCCTGTT CCTGGCCGCC
  421 CGTGAGGGCA GCTATGAGAC TGCCAAAGTG TTGCTGGACC ACTTTGCCAA CCGGGACATC
  481 ACGGATCACA TGGACCGATT GCCGCGGGAC ATCGCACAGG AGCGTATGCA CCACGATATC
  541 GTGCGGCTTT TGGATGAGTA CAACCTGGTG CGCAGCCCAC AGCTGCATGG CACTGCCCTG
  601 GGTGGCACAC CCACTCTGTC TCCCACACTC TGCTCGCCCA ATGGCTACCT GGGCAATCTC
  661 AAGTCTGCCA CACAGGGCAA GAAGGCCCGC AAGCCCAGCA CCAAAGGGCT GGCTTGTGGT
  721 AGCAAGGAAG CTAAGGACCT CAAGGCACGG AGGAAGAAGT CCCAGGATGG CAAGGGCTGC
  781 CTGTTGGACA GCTCGAGCAT GCTGTCGCCT GTGGACTCCC TCGAGTCACC CCATGGCTAC
  841 TTGTCAGATG TGGCCTCGCC ACCCCTCCTC CCCTCCCCAT TCCAGCAGTC TCCATCCATG
  901 CCTCTCAGCC ACCTGCCCTG GTATGCCTGA CACTCACCCT GGGCATCAGC CACTTGAAAT
  961 GTGGCAGCCC AAGCCCTGAG ATGGGCAGCA CTGGGCTGGG AGGGTAGCCG GCTTGGCCTT
 1021 TGAGCCACCC CCCGACACGC TCCTCCCACT GCCTGTAGCT CAGTGCCAGC ACAGTGCTGA
1081 GTACTAATGC ACGGGGCTAT GGATTCACGT GATGACACCG GACTGCAGGA CCT
//
Primer: RDB14500#2 (Pr0430)
Region: insert mid
Sequence file: RDB14500_A6Lcd.seq check
>D04132A1_A6Lc_1_RDB14500#2_B04_02_ABI08.ab1
    1 CTTTAGGGTG GGGAGGGCTG CTGTGTAGTG GCCCCATCAT GCTATGCTGG AGGCCAGCTG
   61 CCTGTGTGCT CAGTGTGCCC GGCGTCACAC CCGGCCGTAG TGGGTTGTAC TGGCTGGGCA
  121 CCATGCCATT CTGGAGCCGG GGAAGCCACT TACACTGGCC ATTCAAGCTT GCCGGTGCAC
  181 CCACGGTGAA ATTCATAGCC CCCGTGCCAT TGGTACTCAG CACTGTGCTG GCACTGGAGG
  241 CTACAGGCAG GTGGGAGAGG CGTGGCGGGG GTGGCTCAAA GGCCAACCGG CTACCTCCAG
  301 CCAGTGCTGC CATCTCAGGC TTGGCTGCCA CATTCAAGTG GCTGATGCCC AGGTGAGTGT
  361 CAGGCATACC AGGCAGGTGG CTGAGAGGCA TGGATGGAGA CTGCTGGAAT GGGGAGGGGA
  421 GGAGGGGTGG CGAGGCCACA TCTGACAAGT AGCCATGGGG TGACTCGAGG GAGTCCACAG
  481 GCGACAGCAT GCTCGAGCTG TCCAACAGGC AGCCCTTGCC ATCCTGGGAC TTCTTCCTCC
  541 GTGCCTTGAG GTCCTTAGCT TCCTTGCTAC CACAAGCCAG CCCTTTGGTG CTGGGCTTGC
  601 GGGCCTTCTT GCCCTGTGTG GCAGACTTGA GATTGCCCAG GTAGCCATTG GGCGAGCAGA
  661 GTGTGGGGAG ACAGAGTGGG TGTGCCACCC AGGGCAGTGC CATGCAGCTG TGGGCTGCGC
  721 ACCAGGGTTG TACTCATCCA AAAAGCCGCA CGATATCGTG GTGCATACGC TCCTGGTGCG
  781 ATGTCCCGCG GCAATCGGTC CATGTGATCC GTGATGTCCC GGTTGGCAAA GTGGTCCAGC
  841 AACACTTTGG GCAGTCTCAT AGCTGCCCTC ACGGGCGGCC AGGAAACAGG GGAGTCTCCT
  901 TCCTTGTTGT TCTGCCATGT CCTTGTTGGC TCCGTTTCTT CAAGGAGCAC AACAGCAGCA
  961 ATCCCAACAT TTGGTTCCAC CCGCGGCCCG CCCAAATGCA AAAGCCCGAA CTTTGGCCCT
 1021 TAGGGTCATC CACGGGCCAT TTGAACGGTT CAAGCAATGG TTGAAGTTGG ATGGAGGGTC
 1081 CCCTTCCAAG CAATTGCTCC TTTCCAACTG GGCCAGGGGC CGCGGCTCGA AGCCAAGGAT
1141 TCCAGGTTGG GAAGTTGTGT GGCCCCATTC CATATGGGAC TAATTCCTGG
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR
Sequence file: RDB14500_A6Lce.seq check
>D04132A1_A6Lc_1_SV40pro_F_V2_C04_03_ABI08.ab1
    1 GTTGCGCCTT TTTTGGAGGA TTGATCAGCT TGGGATCTGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATCTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTCTATGG AAAGGTTGGG
  961 CTTCGGAATT CGTTTTCCGG GACGCCGGCT GGATGGATCC CTCAGCGCGG GGATCTCAAT
 1021 GCTGAAGTTC CTTCGCCCCA CCCCGGCTTC GATCCCTCCG CGAGGTTGGA TCAGCTGCTG
1081 CTGAAGCCTG ACGACCCTCG CGGGAAGTCA T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sasaki, N., The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Development 138 (1): 55-64 (2011). PMID 21098559. [link to RRC of NBRP]