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pmStr-C1-mouse Rab11A-Q70L (#RDB15031)

Expression vector of mouse constitutively active (CA) mutant Rab11A (Q70L), fused with N-terminal mStr, CMV promoter.

Clone info. Expression vector of mouse constitutively active (CA) mutant Rab11A (Q70L), fused with N-terminal mStr, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pmStr-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab11a cDNA
Reference of insert sequence AB232605 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15031 pmStr-C1-mouse Rab11A-Q70L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmStr-C1-mouse Rab11A-Q70L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15031).

Reference section:

Oguchi, M.E., Noguchi, K., Fukuda, M., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15031_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mStr-C_F (Pr0181)
Region: mStr 3', insert (Q70L)
Sequence file: RDB15031_A7Ega.seq check
>D04556G7_A7Eg_2_mStr-C_F_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCN NGGNNNTGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG
   61 CGGCATGGNC GAGCTGTACA AGTCCGGACT CAGATCCATG GGCACCCGCG ACGACGAGTA
  121 CGACTACCTC TTTAAAGTTG TCCTTATTGG AGATTCTGGT GTTGGAAAGA GTAACCTCCT
  181 GTCTCGATTT ACTCGAAATG AGTTTAATCT GGAAAGCAAG AGTACCATTG GAGTAGAGTT
  241 TGCAACAAGA AGCATCCAGG TTGATGGGAA AACAATAAAG GCACAGATAT GGGACACAGC
  301 AGGGCTCGAG CGGTACAGGG CTATAACGTC TGCATACTAT CGTGGAGCAG TAGGTGCCTT
  361 ATTGGTTTAT GACATTGCTA AGCATCTCAC ATATGAAAAT GTAGAGCGAT GGCTGAAAGA
  421 ACTGAGAGAT CATGCTGATA GTAACATTGT TATCATGCTT GTGGGCAATA AGAGTGATTT
  481 ACGTCATCTC AGGGCAGTTC CTACAGATGA AGCAAGAGCT TTTGCAGAGA AGAATGGTTT
  541 GTCATTCATT GAGACATCTG CTCTAGATTC TACAAATGTT GAAGCTGCTT TTCAGACAAT
  601 TCTAACAGAG ATATACCGCA TTGTTTCTCA GAAGCAAATG TCAGACAGAC GTGAAAATGA
  661 CATGTCTCCA AGCAACAATG TGGTTCCTAT TCATGTTCCG CCCACCACTG AGAACAAGCC
  721 AAAGGTGCAG TGCTGTCAGA ACATCTAAAA TCACTAGTGA ATTCGCGGCC GCCTGCAGGT
  781 CGACGGTACC GCGGGCCCGG GATCCACCGG ATCTAGATAA CTGATCATAA TCAGCCATAC
  841 CACATTTGTA GAGGTTTTAC TTGCTTTNAA AAACCTCCCA CACNNNCCCC NGAACNTGAA
  901 ACATAAAATG AATGCAATTG TTGTTGTTAN CTTGTTTATT GNAGCTTATN NTGGNTNCNA
  961 ATAAAGCNNN NGCNNCNNNA NNTTNNCNAA TNAAGCNNTT TTTTTNNNNN NNNTNNNNNN
1021 NNNN
//
Primer: SV40polyA-R2 (Pr0623)
Region: SV40 pA, insert 3' (Q70L)
Sequence file: RDB15031_A7Egb.seq check
>D04556G7_A7Eg_2_SV40polyA_R2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNNNTTAAC AACNACNATT GCATTCNTTT TATGTTTCNG
   61 GTTCNGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACCTG
  181 CAGGCGGCCG CGAATTCACT AGTGATTTTA GATGTTCTGA CAGCACTGCA CCTTTGGCTT
  241 GTTCTCAGTG GTGGGCGGAA CATGAATAGG AACCACATTG TTGCTTGGAG ACATGTCATT
  301 TTCACGTCTG TCTGACATTT GCTTCTGAGA AACAATGCGG TATATCTCTG TTAGAATTGT
  361 CTGAAAAGCA GCTTCAACAT TTGTAGAATC TAGAGCAGAT GTCTCAATGA ATGACAAACC
  421 ATTCTTCTCT GCAAAAGCTC TTGCTTCATC TGTAGGAACT GCCCTGAGAT GACGTAAATC
  481 ACTCTTATTG CCCACAAGCA TGATAACAAT GTTACTATCA GCATGATCTC TCAGTTCTTT
  541 CAGCCATCGC TCTACATTTT CATATGTGAG ATGCTTAGCA ATGTCATAAA CCAATAAGGC
  601 ACCTACTGCT CCACGATAGT ATGCAGACGT TATAGCCCTG TACCGCTCGA GCCCTGCTGT
  661 GTCCCATATC TGTGCCTTTA TTGTTTTCCC ATCAACCTGG ATGCTTCTTG TTGCAAACTC
  721 TACTCCAATG GTACTCTTGC TTTCCAGATT AAACTCATTT CGAGTAAATC GAGACAGGAN
  781 GTTACTCTTT CCAACACCAG AATCTCNNAT AANGACAACT TTAAAGAGGT AGTCGTACTC
  841 GTCGTCGCGG GNGCCCATGG NTCTGANTNC GGANTTGNAC AGCTCGTCCA TGNCGCCNGN
  901 NGANTGGCGG NCCTCNGCGC GTNCGTACNG TTCCNCGANG GTGNANNCCN CGNNGNGGNA
  961 NGNGANGTNC NANTTGATGN NNANNANGNN NNNNCNNGNN NNCTGCNNNG GNNNCNNNNN
1021 NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, mStr 5'
Sequence file: RDB15031_A7Egc.seq check
>D04556G7_A7Eg_2_CMV_Forward_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN NGCNNTGGGC GGTANGCGTG TNCGGNGGGN NGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGAATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA
  181 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT
  241 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT
  301 GGGACATCCT AACCCCCAAC TTCACCTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG
  361 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA
  421 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT
  481 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA
  541 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA
  601 AGGGCGAGAT CAAGATGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGANGTCA
  661 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACATC GTCGGCATCA
  721 AGTTGGACAT CACCTCCCAC AACGAGGANT ACACCATCGT GGAACTGTAC GAACGCGCCG
  781 ANGGCCGCCA CTCCACCGGC GGCNTGGNCG ANCTGTACAA GTCCGGANTC ANATNCNTGG
  841 GNACCCGCGA CGACNANTAC GACTACNNCT TTNANGTTGN NCTTATTGGA NANTCTGGNN
901 NNGGAAANAN TAANCNNCNN NCNCNANTTA NNNNNANNNN NNTNNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB15031_A7Egd.seq check
>D04556G7_A7Eg_2_SV40pro_ori_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCCT CNGCCTCTNN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
  121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
  181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
  241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
  301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
  361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
  421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
  481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
  541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
  601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG
  661 ANGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC
  721 GCTTTTCTGG ANTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAN
  781 CGTTGGCTAN NNGNGATATT GCTGAAGANC TTGGCGGCGA ATGGGCTGAN NGCTNCCTCN
  841 NGCTTTACNG TATCGCCNCT CCCNANNCNC ANCGCATCGC CNTCNATCGN NNNCNNNNNN
901 ANNNNNNNNN ANNNGNNNNN NNGGNNNTNN NAANNGANNN NNNNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oguchi, M.E., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]