Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter.
Clone info. | Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter. |
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Comment | Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAL009C12 (HGY083660). |
Vector backbone | pCAG2-EGFP-C1 (plasmid) |
Size of vector backbone | 5.9 kb |
Selectable markers | Amp^r |
Gene/insert name | human IFT122 cDNA |
Depositor|Developer | Nakayama, Kazuhisa | Katoh, Yohei | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15190 | pCAG2-EGFP-C1-IFT122 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCAG2-EGFP-C1-IFT122 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15190). |
Reference section:
Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15190_A7Fsp1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EGFP-C (Pr0025) Region: EGFP, insert 5' Sequence file: RDB15190_A7Fsa.seq ![]() |
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>D04646A1_A7Fs_1_EGFP-C_A07_01_ABI24.ab1 1 ACGGGTATCT CGGCATGGAC GAGCTGTACA GTCCGGACTC AGATCTCGAG CTCAAGCTTC 61 GAATTCTATG AGGGCCGTGT TGACGTGGAG AGATAAAGCC GAGCACTGTA TAAATGACAT 121 CGCATTTAAG CCTGATGGAA CTCAACTGAT TTTGGCTGCC GGAAGCAGAT TACTGGTTTA 181 TGACACCTCT GATGGCACCT TACTTCAGCC CCTCAAGGGA CACAAAGACA CTGTGTACTG 241 TGTGGCATAT GCGAAGGATG GCAAGCGCTT TGCTTCTGGA TCAGCTGACA AAAGCGTTAT 301 TATCTGGACA TCAAAACTGG AAGGCATTCT GAAGTACACG CACAATGATG CTATACAATG 361 TGTCTCCTAC AATCCTATTA CTCATCAACT GGCATCTTGT TCCTCCAGTG ACTTTGGGTT 421 GTGGTCTCCT GAACAGAAGT CTGTCTCCAA ACACAAATCA AGCAGCAAGA TCATCTGCTG 481 CAGCTGGACA AATGATGGTC AGTACCTGGC GCTGGGGATG TTCAATGGGA TCATCAGCAT 541 ACGGAACAAA AATGGCGAGG AGAAAGTAAA GATCGAGCGG CCGGGGGGCT CCCTCTCGCC 601 AATATGGTCC ATCTGCTGGA ACCCTTCAAG AGAGGAACGT AATGACATCC TGGCTGTGGC 661 TGACTGGGGA CAGAAAGTTT CCTTCTACCA GCTGAGTGGA AAACAGATTG GAAAGGATCG 721 GGCACTGAAC TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGCGAGT ACATTTTGCT 781 GGGGGGTTCA GACAAGCAAG TATCTCTTTT CACCAAGGAT GGAGTGCGGC TTGGGACTGT 841 TGGGGAGCAG AACTCCTGGG TGTGGACGTG TCAAGCGAAA CCGGATTCCA ACTATGTGGT 901 GGTCGGCTGC CAGGACGGCA CCATTTCCCT TCTACCAGCT TATTTTCAGC ACAGTCCATG 961 GGCTTTACAA GGACCGCTAT GCCCTACAGG GATAGCATGA CTGACGTCAT TGTGCAGCAC 1021 CTGATCACTG AGCAGAAAGT TCGGATAATG CTAAGAGCTT GTCAGAGATT GCAATCTACA 1081 GAATCGATGC TATCACTGCT GGAAATCCTC ATCTATGGAG TTGTTATTTC AGGAGGAAAC 1141 TTATTCTCCA // |
Primer: pCAGGS_R1 (Pr0614) Region: beta-glonbinpA,insert 3' Sequence file: RDB15190_A7Fsb.seq ![]() |
>D04646A1_A7Fs_1_pCAGGS_R1_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNCNCNNT TGNNNTGAAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA 61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCCCAGTGG 121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC 181 TGAGGAGTCG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTCA 241 TGGGCCAGGG TCATCCTTGC ACCTGCGGCA GTAGGGGCAG CAGCCATGCT GAAGCACCAG 301 CAACTCATAG TCCTCAGAAT GGAACATCTG GAAGCAGGAG GGGCACATGG TAATGGAGGC 361 GTCAGGCAGC AGTGAGCGGA AGTATTGCCA CCTCAGGGGT GGGGGCCATC GCTTGATGAG 421 GACATCCCGG CGGCTCATGG AGCGCAGCAC CAGCCGGCTC ACCACCACTG GCACGAACTC 481 TGAGCCACCT TGCTCAAAGC TCAGCTTAGC TGTGAACGGG TCCTCATCTC CGATGGAGTC 541 CTTGGTCTCC ACTAGCCGCA GAATCTGGGA GCTGTTGTTT GCAATCTCTA GCTGTCTGTC 601 ATCCCGCTTG GGTCTCAGCA CCTCCAGGTC GATGAGGGAG ATGGCTTCTT CATCAGTGAT 661 CCCTTCCTCC AGGTAGAACT CAACCAGGTG TAGCACGTCG TANGAAGAGG CGGANAAGAN 721 GAAGGGCTGG CGGCAGTTGN TGCAGACGTT GCCCAGGTTG TTGAGCAGCG GGTTGTTGGT 781 GGAGCAGCGG TAGCACAAGG GCACCAACTN CTCACTGTCG TGNAANGGCT TGGCGCGGNT 841 GGTCANGGTA NCCAGCTCAA TGGNNTTTTG GAATCTGGNA NGGNNGTACA GGNCACGCAN 901 CTTGNCATNN NCGNGNCNGN NNNGCCNNNN NNNANNNNNG NNNNNGNNNN GNTNNNNNNA 961 NNNNNNNNNN // |
Primer: EGFP-N (Pr0027) Region: EGFP, CAG pro Sequence file: RDB15190_A7Fsc.seq ![]() |
>D04646A1_A7Fs_1_EGFP-N_A12_03_ABI24.ab1 1 GCACCCCGGG CGACGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTA ATTTGCCAAA 61 ATGATGAGAC AGCACAATAA CCAGCACGTT GCCCAGGAGC TGTAGGAAAA AGAAGAAGGC 121 ATGAACATGG TTAGCAGAGG CTAGAGCCGC CGGTCACACG CCAGAAGCCG AACCCCGCCC 181 TGCCCCGTCC CCCCCGAAGG CAGCCGTCCC CCCGCGGACA GCCCCGAGGC TGGAGAGGGA 241 GAAGGGGACG GCGGCGCGGC GACGCACGAA GGCCCTCCCC GCCCATTTCC TTCCTGCCGG 301 CGCCGCACCG CTTCGCCCCG CGCCCGCTAG AGGGGGTGCG GCGGCGCCTC CCAGATTTCG 361 GCTCCGCACA GATTTGGGAC AAAGGAAGTC CCTGCGCCCT CTCGCACGAT TACCATAAAA 421 GGCAATGGCT GCGGCTCGCC GCGCCTCGAC AGCCGCCGGC GCTCCGGGGG CCGCCGCGCC 481 CCTCCCCCGA GCCCTCCCCG GCCCGAGGCG GCCCCGCCCC GCCCGGCACC CCCACCTGCC 541 GCCACCCCCC GCCCGGCACG GCGAGCCCCG CGCCACGCCC CGTACGGAGC CCCGCACCCG 601 AAGCCGGGCC GTGCTCAGCA ACTCGGGGAG GGGGGTGCAG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
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