Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG2-EGFP-C1-IFT122 (#RDB15190)

Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter.

Clone info. Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter.
Comment Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAL009C12 (HGY083660).
Vector backbone pCAG2-EGFP-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name human IFT122 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15190 pCAG2-EGFP-C1-IFT122 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG2-EGFP-C1-IFT122 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15190).

Reference section:

Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15190_A7Fsp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025)
Region: EGFP, insert 5'
Sequence file: RDB15190_A7Fsa.seq check
>D04646A1_A7Fs_1_EGFP-C_A07_01_ABI24.ab1
    1 ACGGGTATCT CGGCATGGAC GAGCTGTACA GTCCGGACTC AGATCTCGAG CTCAAGCTTC
   61 GAATTCTATG AGGGCCGTGT TGACGTGGAG AGATAAAGCC GAGCACTGTA TAAATGACAT
  121 CGCATTTAAG CCTGATGGAA CTCAACTGAT TTTGGCTGCC GGAAGCAGAT TACTGGTTTA
  181 TGACACCTCT GATGGCACCT TACTTCAGCC CCTCAAGGGA CACAAAGACA CTGTGTACTG
  241 TGTGGCATAT GCGAAGGATG GCAAGCGCTT TGCTTCTGGA TCAGCTGACA AAAGCGTTAT
  301 TATCTGGACA TCAAAACTGG AAGGCATTCT GAAGTACACG CACAATGATG CTATACAATG
  361 TGTCTCCTAC AATCCTATTA CTCATCAACT GGCATCTTGT TCCTCCAGTG ACTTTGGGTT
  421 GTGGTCTCCT GAACAGAAGT CTGTCTCCAA ACACAAATCA AGCAGCAAGA TCATCTGCTG
  481 CAGCTGGACA AATGATGGTC AGTACCTGGC GCTGGGGATG TTCAATGGGA TCATCAGCAT
  541 ACGGAACAAA AATGGCGAGG AGAAAGTAAA GATCGAGCGG CCGGGGGGCT CCCTCTCGCC
  601 AATATGGTCC ATCTGCTGGA ACCCTTCAAG AGAGGAACGT AATGACATCC TGGCTGTGGC
  661 TGACTGGGGA CAGAAAGTTT CCTTCTACCA GCTGAGTGGA AAACAGATTG GAAAGGATCG
  721 GGCACTGAAC TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGCGAGT ACATTTTGCT
  781 GGGGGGTTCA GACAAGCAAG TATCTCTTTT CACCAAGGAT GGAGTGCGGC TTGGGACTGT
  841 TGGGGAGCAG AACTCCTGGG TGTGGACGTG TCAAGCGAAA CCGGATTCCA ACTATGTGGT
  901 GGTCGGCTGC CAGGACGGCA CCATTTCCCT TCTACCAGCT TATTTTCAGC ACAGTCCATG
  961 GGCTTTACAA GGACCGCTAT GCCCTACAGG GATAGCATGA CTGACGTCAT TGTGCAGCAC
 1021 CTGATCACTG AGCAGAAAGT TCGGATAATG CTAAGAGCTT GTCAGAGATT GCAATCTACA
 1081 GAATCGATGC TATCACTGCT GGAAATCCTC ATCTATGGAG TTGTTATTTC AGGAGGAAAC
1141 TTATTCTCCA
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-glonbinpA,insert 3'
Sequence file: RDB15190_A7Fsb.seq check
>D04646A1_A7Fs_1_pCAGGS_R1_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNCNCNNT TGNNNTGAAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA
   61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCCCAGTGG
  121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
  181 TGAGGAGTCG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTCA
  241 TGGGCCAGGG TCATCCTTGC ACCTGCGGCA GTAGGGGCAG CAGCCATGCT GAAGCACCAG
  301 CAACTCATAG TCCTCAGAAT GGAACATCTG GAAGCAGGAG GGGCACATGG TAATGGAGGC
  361 GTCAGGCAGC AGTGAGCGGA AGTATTGCCA CCTCAGGGGT GGGGGCCATC GCTTGATGAG
  421 GACATCCCGG CGGCTCATGG AGCGCAGCAC CAGCCGGCTC ACCACCACTG GCACGAACTC
  481 TGAGCCACCT TGCTCAAAGC TCAGCTTAGC TGTGAACGGG TCCTCATCTC CGATGGAGTC
  541 CTTGGTCTCC ACTAGCCGCA GAATCTGGGA GCTGTTGTTT GCAATCTCTA GCTGTCTGTC
  601 ATCCCGCTTG GGTCTCAGCA CCTCCAGGTC GATGAGGGAG ATGGCTTCTT CATCAGTGAT
  661 CCCTTCCTCC AGGTAGAACT CAACCAGGTG TAGCACGTCG TANGAAGAGG CGGANAAGAN
  721 GAAGGGCTGG CGGCAGTTGN TGCAGACGTT GCCCAGGTTG TTGAGCAGCG GGTTGTTGGT
  781 GGAGCAGCGG TAGCACAAGG GCACCAACTN CTCACTGTCG TGNAANGGCT TGGCGCGGNT
  841 GGTCANGGTA NCCAGCTCAA TGGNNTTTTG GAATCTGGNA NGGNNGTACA GGNCACGCAN
  901 CTTGNCATNN NCGNGNCNGN NNNGCCNNNN NNNANNNNNG NNNNNGNNNN GNTNNNNNNA
961 NNNNNNNNNN
//
Primer: EGFP-N (Pr0027)
Region: EGFP, CAG pro
Sequence file: RDB15190_A7Fsc.seq check
>D04646A1_A7Fs_1_EGFP-N_A12_03_ABI24.ab1
    1 GCACCCCGGG CGACGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTA ATTTGCCAAA
   61 ATGATGAGAC AGCACAATAA CCAGCACGTT GCCCAGGAGC TGTAGGAAAA AGAAGAAGGC
  121 ATGAACATGG TTAGCAGAGG CTAGAGCCGC CGGTCACACG CCAGAAGCCG AACCCCGCCC
  181 TGCCCCGTCC CCCCCGAAGG CAGCCGTCCC CCCGCGGACA GCCCCGAGGC TGGAGAGGGA
  241 GAAGGGGACG GCGGCGCGGC GACGCACGAA GGCCCTCCCC GCCCATTTCC TTCCTGCCGG
  301 CGCCGCACCG CTTCGCCCCG CGCCCGCTAG AGGGGGTGCG GCGGCGCCTC CCAGATTTCG
  361 GCTCCGCACA GATTTGGGAC AAAGGAAGTC CCTGCGCCCT CTCGCACGAT TACCATAAAA
  421 GGCAATGGCT GCGGCTCGCC GCGCCTCGAC AGCCGCCGGC GCTCCGGGGG CCGCCGCGCC
  481 CCTCCCCCGA GCCCTCCCCG GCCCGAGGCG GCCCCGCCCC GCCCGGCACC CCCACCTGCC
  541 GCCACCCCCC GCCCGGCACG GCGAGCCCCG CGCCACGCCC CGTACGGAGC CCCGCACCCG
601 AAGCCGGGCC GTGCTCAGCA ACTCGGGGAG GGGGGTGCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]