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pCAG2-EGFP-C1-IFT140 (#RDB15192)

Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter.

Clone info. Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter.
Comment Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAK069L05 (HGX027869).
Vector backbone pCAG2-EGFP-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name human IFT140 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15192 pCAG2-EGFP-C1-IFT140 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG2-EGFP-C1-IFT140 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15192).

Reference section:

Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15192_A7Fsp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025)
Region: EGFP, insert 5'
Sequence file: RDB15192_A7Fsa.seq check
>D04646A3_A7Fs_1_EGFP-C_B12_06_ABI24.ab1
    1 CAGGGAATCT CGGCATGGAC GAGCTGTACA AGTCCGGACT CAGATCTCGA GCTCAAGCTT
   61 CGAATTCTAT GGCCCTCTAT TATGACCACC AGATAGAAGC CCCGGATGCA GCAGGGTCAC
  121 CCTCATTTAT CAGCTGGCAC CCTGTCCATC CATTCTTGGC AGTTGCTTAC ATCAGCACAA
  181 CCTCAACAGG CAGCGTGGAT ATTTACCTGG AGCAAGGGGA GTGCGTGCCA GATACACACG
  241 TCGAGAGGCC GTTCCGGGTT GCTTCCCTGT GCTGGCACCC GACGCGGCTG GTGCTGGCTG
  301 TGGGCTGGGA GACTGGAGAA GTGACGGTGT TTAACAAGCA GGACAAGGAG CAGCACACGA
  361 TGCCCCTGAC ACACACAGCC GACATCACCG TGCTCCGTTG GAGCCCCAGT GGAAACTGCC
  421 TGCTGTCTGG GGACAGGCTT GGTGTCTTGC TCTTGTGGAG GTTGGACCAA AGGGGCCGAG
  481 TGCAAGGGAC GCCTCTGCTG AAACACGAGT ATGGGAAACA CCTCACGCAC TGCATCTTCC
  541 GGCTCCCCCC TCCTGGCGAG GACCTGGTTC AGTTGGCAAA GGCAGCTGTG AGCGGTGATG
  601 AGAAAGCCCT GGACATGTTT AACTGGAAGA AGAGCAGTTC TGGAAGTTTG CTGAAGATGG
  661 GGTCTCACGA GGGGCTGTTG TTCTTTGTCA GTCTGATGGA CGGGACAGTG CACTATGTGG
  721 ATGAGAAGGG CAAGACCACT CAGGTGGTGT CCGCAGACAG CACGATTCAG ATGCTGTTCT
  781 ACATGGAGAA GAGGGAGGCA CTGGTGGTGG TCACAGAGAA CCTCCGGCTG TCCCTGTACA
  841 CGGTGCCTCC TGAGGGCAAA GCAGAAGAAG TGATGAAGGT CAAGCTGAGC GGGAAAACCG
  901 GCCGCCGGGC AGACATCGCT TTGATTGAAA GGCAGCCTTC TCGTGATGGC CGTCGGGGAG
  961 GCTGCCCTCA GATTCTGGGA CATAGAACGA GGGAGAGAAT TATATACTGA GTTCCAGATG
 1021 AGAAGTTTGG CTTTGAGAAG GAGAGGATTA TTGAACTTGT TGTGTGTTAC TGTAAGTCAA
 1081 CGGTTCTTTC TGACGCTGTA CGACCGAAGG CCGAGTTAGC CCATGGGTGG GAGGGAAAAA
1141 GT
//
Primer: pCA-bgter (Pr0066)
Region: beta-glonbinpA,insert 3'
Sequence file: RDB15192_A7Fsb.seq check
>D04646A3_A7Fs_1_pCAGGS_R1_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNNCNCTNT TGNNNTGNAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA
   61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCCCAGTGG
  121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
  181 TGAGGAGTCG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTCA
  241 GGGGTCGTCA TCTGCCTCTT CCACCACCTC CTCGTCCAGC TCCCTGGCGT CCTCCATGCT
  301 GTTGTGGCGG ACCTGCTCGG GGACGGTGCG TGGCAGTGGG AGACCCAGCC CCCGGTGCAC
  361 GGCGTCCACG GCCTGCGGGC TCACGTAGTA GGACATGTTG GCCAAGGGAA GCCGCCGCCG
  421 CATCTCCTCC AGGAATCTGT AGGCCGTCTG GTATTCCTCC TTCCGCACGT AGTGCTCCAC
  481 CAGGAAGCCA TAGACGTCCC CGATGCGGAT GGTGCTGTCC AGGTCTGGTT CCTCCAGGAG
  541 CAGCTCACAC TGCTTGATGG ACTCCTTGGG GTCCTCTGTG TACGTCCTGC GGGCCTGGAT
  601 GAACCTCTTC ACCAGTGCCA TCCTGCTCTG CAGCTGCGCC AGCCTGGTCT CCTGGTCCAG
  661 GGGGCTCTTG GCCTTGGCCT TGGCCAGGCA CTTGTAGGCC TCGGTCAGCG CCCCGTGGGC
  721 TTTGTCGTAG TTCTGGTATT CATCAATCTC CACCTGGGCA CAAGCGTCNT AAAAGCCAGC
  781 CAGGANGTCC AGGGCCCGCC CCTTGGTGTA GAAGCCGATG ANGTTCTTCA TGATCTCCGG
  841 CTCCTTCCGC CAGTCCAGNN NNTGCAGGTA GTTAGCAGCC ATGATGTANA TTTCCTTCTG
  901 NCNNGNNACG CTCGCGAANA ACGNNNNTTT CTCCNNGNCT CCNGNNTTGA NCANCGCCNN
961 CNTNGNNNNT CANCTNNNTN NNNNGNNNNN NNNNN
//
Primer: EGFP-N (Pr0027)
Region: EGFP, CAG pro
Sequence file: RDB15192_A7Fsc.seq check
>D04646A3_A7Fs_1_EGFP-N_C08_08_ABI24.ab1
    1 GGCCCCCGGG GGGACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT AATTTGCCAA
   61 AATGATGAGA CAGCACAATA ACCAGCACGT TGCCCAGGAG CTGTAGGAAA AAGAAGAAGG
  121 CATGAACATG GTTAGCAGAG GCTAGAGCCG CCGGTCACAC GCCAGAAGCC GAACCCCGCC
  181 CTGCCCCGTC CCCCCCGAAG GCAGCCGTCC CCCCGCGGAC AGCCCCGAGG CTGGAGAGGG
  241 AGAAGGGGAC GGCGGCGCGG CGACGCACGA AGGCCCTCCC CGCCCATTTC CTTCCTGCCG
  301 GCGCCGCACC GCTTCGCCCC GCGCCCGCTA GAGGGGGTGC GGCGGCGCCT CCCAGATTTC
  361 GGCTCCGCAC AGATTTGGGA CAAAGGAAGT CCCTGCGCCC TCTCGCACGA TTACCATAAA
  421 AGGCAATGGC TGCGGCTCGC CGCGCCTCGA CAGCCGCCGG CGCTCCGGGG GCCGCCGCGC
  481 CCCTCCCCCG AGCCCTCCCC GGCCCGAGGC GGCCCCGCCC CGCCCGGCAC CCCCACCTGC
  541 CGCCACCCCC CGCCCGGCAC GGCGAGCCCC GCGCCACGCC CCGTACGGAG CCCCGCACCC
601 GAAGCCGGGC CGTGCTCAGC AACTCGGGGA GGGGGGTGCA G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]