Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter.
Clone info. | Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter. |
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Comment | Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAK069L05 (HGX027869). |
Vector backbone | pCAG2-EGFP-C1 (plasmid) |
Size of vector backbone | 5.9 kb |
Selectable markers | Amp^r |
Gene/insert name | human IFT140 cDNA |
Depositor|Developer | Nakayama, Kazuhisa | Katoh, Yohei | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15192 | pCAG2-EGFP-C1-IFT140 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCAG2-EGFP-C1-IFT140 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15192). |
Reference section:
Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15192_A7Fsp1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EGFP-C (Pr0025) Region: EGFP, insert 5' Sequence file: RDB15192_A7Fsa.seq ![]() |
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>D04646A3_A7Fs_1_EGFP-C_B12_06_ABI24.ab1 1 CAGGGAATCT CGGCATGGAC GAGCTGTACA AGTCCGGACT CAGATCTCGA GCTCAAGCTT 61 CGAATTCTAT GGCCCTCTAT TATGACCACC AGATAGAAGC CCCGGATGCA GCAGGGTCAC 121 CCTCATTTAT CAGCTGGCAC CCTGTCCATC CATTCTTGGC AGTTGCTTAC ATCAGCACAA 181 CCTCAACAGG CAGCGTGGAT ATTTACCTGG AGCAAGGGGA GTGCGTGCCA GATACACACG 241 TCGAGAGGCC GTTCCGGGTT GCTTCCCTGT GCTGGCACCC GACGCGGCTG GTGCTGGCTG 301 TGGGCTGGGA GACTGGAGAA GTGACGGTGT TTAACAAGCA GGACAAGGAG CAGCACACGA 361 TGCCCCTGAC ACACACAGCC GACATCACCG TGCTCCGTTG GAGCCCCAGT GGAAACTGCC 421 TGCTGTCTGG GGACAGGCTT GGTGTCTTGC TCTTGTGGAG GTTGGACCAA AGGGGCCGAG 481 TGCAAGGGAC GCCTCTGCTG AAACACGAGT ATGGGAAACA CCTCACGCAC TGCATCTTCC 541 GGCTCCCCCC TCCTGGCGAG GACCTGGTTC AGTTGGCAAA GGCAGCTGTG AGCGGTGATG 601 AGAAAGCCCT GGACATGTTT AACTGGAAGA AGAGCAGTTC TGGAAGTTTG CTGAAGATGG 661 GGTCTCACGA GGGGCTGTTG TTCTTTGTCA GTCTGATGGA CGGGACAGTG CACTATGTGG 721 ATGAGAAGGG CAAGACCACT CAGGTGGTGT CCGCAGACAG CACGATTCAG ATGCTGTTCT 781 ACATGGAGAA GAGGGAGGCA CTGGTGGTGG TCACAGAGAA CCTCCGGCTG TCCCTGTACA 841 CGGTGCCTCC TGAGGGCAAA GCAGAAGAAG TGATGAAGGT CAAGCTGAGC GGGAAAACCG 901 GCCGCCGGGC AGACATCGCT TTGATTGAAA GGCAGCCTTC TCGTGATGGC CGTCGGGGAG 961 GCTGCCCTCA GATTCTGGGA CATAGAACGA GGGAGAGAAT TATATACTGA GTTCCAGATG 1021 AGAAGTTTGG CTTTGAGAAG GAGAGGATTA TTGAACTTGT TGTGTGTTAC TGTAAGTCAA 1081 CGGTTCTTTC TGACGCTGTA CGACCGAAGG CCGAGTTAGC CCATGGGTGG GAGGGAAAAA 1141 GT // |
Primer: pCA-bgter (Pr0066) Region: beta-glonbinpA,insert 3' Sequence file: RDB15192_A7Fsb.seq ![]() |
>D04646A3_A7Fs_1_pCAGGS_R1_E08_14_ABI24.ab1 1 NNNNNNNNNN NNNNCNCTNT TGNNNTGNAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA 61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCCCAGTGG 121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC 181 TGAGGAGTCG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTCA 241 GGGGTCGTCA TCTGCCTCTT CCACCACCTC CTCGTCCAGC TCCCTGGCGT CCTCCATGCT 301 GTTGTGGCGG ACCTGCTCGG GGACGGTGCG TGGCAGTGGG AGACCCAGCC CCCGGTGCAC 361 GGCGTCCACG GCCTGCGGGC TCACGTAGTA GGACATGTTG GCCAAGGGAA GCCGCCGCCG 421 CATCTCCTCC AGGAATCTGT AGGCCGTCTG GTATTCCTCC TTCCGCACGT AGTGCTCCAC 481 CAGGAAGCCA TAGACGTCCC CGATGCGGAT GGTGCTGTCC AGGTCTGGTT CCTCCAGGAG 541 CAGCTCACAC TGCTTGATGG ACTCCTTGGG GTCCTCTGTG TACGTCCTGC GGGCCTGGAT 601 GAACCTCTTC ACCAGTGCCA TCCTGCTCTG CAGCTGCGCC AGCCTGGTCT CCTGGTCCAG 661 GGGGCTCTTG GCCTTGGCCT TGGCCAGGCA CTTGTAGGCC TCGGTCAGCG CCCCGTGGGC 721 TTTGTCGTAG TTCTGGTATT CATCAATCTC CACCTGGGCA CAAGCGTCNT AAAAGCCAGC 781 CAGGANGTCC AGGGCCCGCC CCTTGGTGTA GAAGCCGATG ANGTTCTTCA TGATCTCCGG 841 CTCCTTCCGC CAGTCCAGNN NNTGCAGGTA GTTAGCAGCC ATGATGTANA TTTCCTTCTG 901 NCNNGNNACG CTCGCGAANA ACGNNNNTTT CTCCNNGNCT CCNGNNTTGA NCANCGCCNN 961 CNTNGNNNNT CANCTNNNTN NNNNGNNNNN NNNNN // |
Primer: EGFP-N (Pr0027) Region: EGFP, CAG pro Sequence file: RDB15192_A7Fsc.seq ![]() |
>D04646A3_A7Fs_1_EGFP-N_C08_08_ABI24.ab1 1 GGCCCCCGGG GGGACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT AATTTGCCAA 61 AATGATGAGA CAGCACAATA ACCAGCACGT TGCCCAGGAG CTGTAGGAAA AAGAAGAAGG 121 CATGAACATG GTTAGCAGAG GCTAGAGCCG CCGGTCACAC GCCAGAAGCC GAACCCCGCC 181 CTGCCCCGTC CCCCCCGAAG GCAGCCGTCC CCCCGCGGAC AGCCCCGAGG CTGGAGAGGG 241 AGAAGGGGAC GGCGGCGCGG CGACGCACGA AGGCCCTCCC CGCCCATTTC CTTCCTGCCG 301 GCGCCGCACC GCTTCGCCCC GCGCCCGCTA GAGGGGGTGC GGCGGCGCCT CCCAGATTTC 361 GGCTCCGCAC AGATTTGGGA CAAAGGAAGT CCCTGCGCCC TCTCGCACGA TTACCATAAA 421 AGGCAATGGC TGCGGCTCGC CGCGCCTCGA CAGCCGCCGG CGCTCCGGGG GCCGCCGCGC 481 CCCTCCCCCG AGCCCTCCCC GGCCCGAGGC GGCCCCGCCC CGCCCGGCAC CCCCACCTGC 541 CGCCACCCCC CGCCCGGCAC GGCGAGCCCC GCGCCACGCC CCGTACGGAG CCCCGCACCC 601 GAAGCCGGGC CGTGCTCAGC AACTCGGGGA GGGGGGTGCA G // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
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