Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter.
Clone info. | Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter. |
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Comment | Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAK069L05 (HGX027869). |
Vector backbone | pCAG2-mCherry-C1 (plasmid) |
Size of vector backbone | 5.9 kb |
Selectable markers | Amp^r |
Gene/insert name | human IFT140 cDNA |
Depositor|Developer | Nakayama, Kazuhisa | Katoh, Yohei | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15193 | pCAG2-mCherry-C1-IFT140 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCAG2-mCherry-C1-IFT140 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15193). |
Reference section:
Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15193_A7Fsp1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: mRFP1-inner1 (Pr0443) Region: mCherry, insert 5' Sequence file: RDB15193_A7Fsa.seq ![]() |
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>D04646A4_A7Fs_2_mRFP1-inner1_E12_15_ABI24.ab1 1 CGCGGGCGGG CGGACGTGAC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG 61 TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG 121 GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA 181 AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA 241 AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA 301 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT 361 CCACCGGCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT CAAGCTTCGA 421 ATTCTATGGC CCTCTATTAT GACCACCAGA TAGAAGCCCC GGATGCAGCA GGGTCACCCT 481 CATTTATCAG CTGGCACCCT GTCCATCCAT TCTTGGCAGT TGCTTACATC AGCACAACCT 541 CAACAGGCAG CGTGGATATT TACCTGGAGC AAGGGGAGTG CGTGCCAGAT ACACACGTCG 601 AGAGGCCGTT CCGGGTTGCT TCCCTGTGCT GGCACCCGAC GCGGCTGGTG CTGGCTGTGG 661 GCTGGGAGAC TGGAGAAGTG ACGGTGTTTA ACAAGCAGGA CAAGGAGCAG CACACGATGC 721 CCCTGACACA CACAGCCGAC ATCACCGTGC TCCGTTGGAG CCCCAGTGGA AACTGCCTGC 781 TGTCTGGGGA CAGGCTTGGT TGTCTTGCTC TTGGTGGAGG TTGACCAAGG GGCCGAGTGC 841 AAGGGACGCC CTCTGCTGAA ACACGAGTAT GGAAACACCT CACGCCACTG CATCCTCGCT 901 CCCTCCCTCC TGGCGAGACT GTCAGTGCAA GCAGCTGTGG ACCGTGATGA AAAGCCCTGA 961 CATGTTAACC TGAGAAGAGC AGTTCTGGAG TTGCCTGAAT GGTTCCCGAA GCTGTGTCTT 1021 GCAGTCTGAT GACCGACACT // |
Primer: pCAGGS_R1 (Pr0614) Region: beta-globinpA,insert 3' Sequence file: RDB15193_A7Fsb.seq ![]() |
>D04646A4_A7Fs_2_pCAGGS_R1_G08_20_ABI24.ab1 1 NNNNNNNNNN NNNNNCACNN TTGNNNTGNA NNNAAATTTC CTTTATTAGC CAGAAGTCAG 61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCCCAGTG 121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC 181 CTGAGGAGTC TATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTC 241 AGGGGTCGTC ATCTGCCTCT TCCACCACCT CCTCGTCCAG CTCCCTGGCG TCCTCCATGC 301 TGTTGTGGCG GACCTGCTCG GGGACGGTGC GTGGCAGTGG GAGACCCAGC CCCCGGTGCA 361 CGGCGTCCAC GGCCTGCGGG CTCACGTAGT AGGACATGTT GGCCAAGGGA AGCCGCCGCC 421 GCATCTCCTC CAGGAATCTG TAGGCCGTCT GGTATTCCTC CTTCCGCACG TAGTGCTCCA 481 CCAGGAAGCC ATAGACGTCC CCGATGCGGA TGGTGCTGTC CAGGTCTGGT TCCTCCAGGA 541 GCAGCTCACA CTGCTTGATG GACTCCTTGG GGTCCTCTGT GTACGTCCTG CGGGCCTGGA 601 TGAACCTCTT CACCAGTGCC ATCCTGCTCT GCAGCTGCGC CAGCCTGGTC TCCTGGTCCA 661 GGGGGCTCTT GGCCTTGGCC TTGGCCAGGC ACTTGTAGGC CTCGGTCAGC GCCCCGTGGG 721 CTTTGTCGTA GTTCTGGTAT TCATCAATCT CCACCTGGGC ACAAGCGTCA TAAAAGCCAG 781 CCAGGAGGTC CAGGGCCCGC CCCTTGGTGT AGAAGCCGAT GANGTTCTTC ATGATCTCCG 841 GCTCCTTCCG CCAGTCCAGG GNNTGCANGT AGTTAGCAGC CATGATGTAN ANTNNCNTCT 901 GCCNNNNNAC GCTCGCGAAN ANNGTGATTT TCNNCGNGNN TNCNGNNTNG AGCAGCGCCN 961 NNNTNGNNNN NCANCTNNNN GNNNGNNNNC NNNNANNTNN NTNNNNNGNN NNNGNNNNNN 1021 NNNN // |
Primer: Red-N_R (Pr0329) Region: mCherry, CAG pro Sequence file: RDB15193_A7Fsc.seq ![]() |
>D04646A4_A7Fs_2_Red-N_R_F12_18_ABI24.ab1 1 GATTAATATG CACTTGAGCG CATGACTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT 61 TGCTCACCAT GGTGGCGCTA GCATTTGCCA AAATGATGAG ACAGCACAAT AACCAGCACG 121 TTGCCCAGGA GCTGTAGGAA AAAGAAGAAG GCATGAACAT GGTTAGCAGA GGCTAGAGCC 181 GCCGGTCACA CGCCAGAAGC CGAACCCCGC CCTGCCCCGT CCCCCCCGAA GGCAGCCGTC 241 CCCCCGCGGA CAGCCCCGAG GCTGGAGAGG GAGAAGGGGA CGGCGGCGCG GCGACGCACG 301 AAGGCCCTCC CCGCCCATTT CCTTCCTGCC GGCGCCGCAC CGCTTCGCCC CGCGCCCGCT 361 AGAGGGGGTG CGGCGGCGCC TCCCAGATTT CGGCTCCGCA CAGATTTGGG ACAAAGGAAG 421 TCCCTGCGCC CTCTCGCACG ATTACCATAA AAGGCAATGG CTGCGGCTCG CCGCGCCTCG 481 ACAGCCGCCG GCGCTCCGGG GGCCGCCGCG CCCCTCCCCC GAGCCCTCCC CGGCCCGAGG 541 CGGCCCCGCC CCGCCCGGCA CCCCCACCTG CCGCCACCCC CCGCCCGGCA CGGCGAGCCC 601 CGCGCCACGC CCCGTACGGA GCCCCGCACC CGAAGCCGGG CCGTGCTCAG CAACTCGGGG 661 AGGGGGGTGC AG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
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