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pCAG2-mCherry-C1-IFT140 (#RDB15193)

Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter.

Clone info. Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter.
Comment Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAK069L05 (HGX027869).
Vector backbone pCAG2-mCherry-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name human IFT140 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15193 pCAG2-mCherry-C1-IFT140 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG2-mCherry-C1-IFT140 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15193).

Reference section:

Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15193_A7Fsp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mRFP1-inner1 (Pr0443)
Region: mCherry, insert 5'
Sequence file: RDB15193_A7Fsa.seq check
>D04646A4_A7Fs_2_mRFP1-inner1_E12_15_ABI24.ab1
    1 CGCGGGCGGG CGGACGTGAC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG
   61 TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG
  121 GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA
  181 AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA
  241 AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA
  301 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
  361 CCACCGGCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT CAAGCTTCGA
  421 ATTCTATGGC CCTCTATTAT GACCACCAGA TAGAAGCCCC GGATGCAGCA GGGTCACCCT
  481 CATTTATCAG CTGGCACCCT GTCCATCCAT TCTTGGCAGT TGCTTACATC AGCACAACCT
  541 CAACAGGCAG CGTGGATATT TACCTGGAGC AAGGGGAGTG CGTGCCAGAT ACACACGTCG
  601 AGAGGCCGTT CCGGGTTGCT TCCCTGTGCT GGCACCCGAC GCGGCTGGTG CTGGCTGTGG
  661 GCTGGGAGAC TGGAGAAGTG ACGGTGTTTA ACAAGCAGGA CAAGGAGCAG CACACGATGC
  721 CCCTGACACA CACAGCCGAC ATCACCGTGC TCCGTTGGAG CCCCAGTGGA AACTGCCTGC
  781 TGTCTGGGGA CAGGCTTGGT TGTCTTGCTC TTGGTGGAGG TTGACCAAGG GGCCGAGTGC
  841 AAGGGACGCC CTCTGCTGAA ACACGAGTAT GGAAACACCT CACGCCACTG CATCCTCGCT
  901 CCCTCCCTCC TGGCGAGACT GTCAGTGCAA GCAGCTGTGG ACCGTGATGA AAAGCCCTGA
  961 CATGTTAACC TGAGAAGAGC AGTTCTGGAG TTGCCTGAAT GGTTCCCGAA GCTGTGTCTT
1021 GCAGTCTGAT GACCGACACT
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globinpA,insert 3'
Sequence file: RDB15193_A7Fsb.seq check
>D04646A4_A7Fs_2_pCAGGS_R1_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNCACNN TTGNNNTGNA NNNAAATTTC CTTTATTAGC CAGAAGTCAG
   61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCCCAGTG
  121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC
  181 CTGAGGAGTC TATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTC
  241 AGGGGTCGTC ATCTGCCTCT TCCACCACCT CCTCGTCCAG CTCCCTGGCG TCCTCCATGC
  301 TGTTGTGGCG GACCTGCTCG GGGACGGTGC GTGGCAGTGG GAGACCCAGC CCCCGGTGCA
  361 CGGCGTCCAC GGCCTGCGGG CTCACGTAGT AGGACATGTT GGCCAAGGGA AGCCGCCGCC
  421 GCATCTCCTC CAGGAATCTG TAGGCCGTCT GGTATTCCTC CTTCCGCACG TAGTGCTCCA
  481 CCAGGAAGCC ATAGACGTCC CCGATGCGGA TGGTGCTGTC CAGGTCTGGT TCCTCCAGGA
  541 GCAGCTCACA CTGCTTGATG GACTCCTTGG GGTCCTCTGT GTACGTCCTG CGGGCCTGGA
  601 TGAACCTCTT CACCAGTGCC ATCCTGCTCT GCAGCTGCGC CAGCCTGGTC TCCTGGTCCA
  661 GGGGGCTCTT GGCCTTGGCC TTGGCCAGGC ACTTGTAGGC CTCGGTCAGC GCCCCGTGGG
  721 CTTTGTCGTA GTTCTGGTAT TCATCAATCT CCACCTGGGC ACAAGCGTCA TAAAAGCCAG
  781 CCAGGAGGTC CAGGGCCCGC CCCTTGGTGT AGAAGCCGAT GANGTTCTTC ATGATCTCCG
  841 GCTCCTTCCG CCAGTCCAGG GNNTGCANGT AGTTAGCAGC CATGATGTAN ANTNNCNTCT
  901 GCCNNNNNAC GCTCGCGAAN ANNGTGATTT TCNNCGNGNN TNCNGNNTNG AGCAGCGCCN
  961 NNNTNGNNNN NCANCTNNNN GNNNGNNNNC NNNNANNTNN NTNNNNNGNN NNNGNNNNNN
1021 NNNN
//
Primer: Red-N_R (Pr0329)
Region: mCherry, CAG pro
Sequence file: RDB15193_A7Fsc.seq check
>D04646A4_A7Fs_2_Red-N_R_F12_18_ABI24.ab1
    1 GATTAATATG CACTTGAGCG CATGACTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT
   61 TGCTCACCAT GGTGGCGCTA GCATTTGCCA AAATGATGAG ACAGCACAAT AACCAGCACG
  121 TTGCCCAGGA GCTGTAGGAA AAAGAAGAAG GCATGAACAT GGTTAGCAGA GGCTAGAGCC
  181 GCCGGTCACA CGCCAGAAGC CGAACCCCGC CCTGCCCCGT CCCCCCCGAA GGCAGCCGTC
  241 CCCCCGCGGA CAGCCCCGAG GCTGGAGAGG GAGAAGGGGA CGGCGGCGCG GCGACGCACG
  301 AAGGCCCTCC CCGCCCATTT CCTTCCTGCC GGCGCCGCAC CGCTTCGCCC CGCGCCCGCT
  361 AGAGGGGGTG CGGCGGCGCC TCCCAGATTT CGGCTCCGCA CAGATTTGGG ACAAAGGAAG
  421 TCCCTGCGCC CTCTCGCACG ATTACCATAA AAGGCAATGG CTGCGGCTCG CCGCGCCTCG
  481 ACAGCCGCCG GCGCTCCGGG GGCCGCCGCG CCCCTCCCCC GAGCCCTCCC CGGCCCGAGG
  541 CGGCCCCGCC CCGCCCGGCA CCCCCACCTG CCGCCACCCC CCGCCCGGCA CGGCGAGCCC
  601 CGCGCCACGC CCCGTACGGA GCCCCGCACC CGAAGCCGGG CCGTGCTCAG CAACTCGGGG
661 AGGGGGGTGC AG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]