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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-TULP3 (#RDB15194)

Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal EGFP, CMV promoter.

Clone info. Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAK069G14 (HGX027758).
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name human TULP3 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15194 pEGFP-C1-TULP3 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-TULP3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15194).

Reference section:

Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15194_B3Gkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert 5'
Sequence file: RDB15194_B3Gka.seq check
>D04646A5_B3Gk_1_mVenus-M_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGCN TCNAGGTNNN NTTNNAGATC CGCCNCAACA TCGAGGNCGG
   61 CAGCGTGCAN NNNNNNNNNN NCTACCAGCA GAACACCCCC ATCGGCGACG GCCCCNNGCT
  121 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
  181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
  241 CGAGCTGTAC AAGTCCGGAC TCAGATCTAT GGAGGCTTCG CGCTGCCGGC TCAGTCCCAG
  301 CGGCGACAGT GTCTTCCATG AAGAAATGAT GAAGATGCGA CAGGCTAAGC TGGATTATCA
  361 GAGGCTACTA CTTGAGAAGA GGCAAAGGAA AAAGCGCCTT GAGCCATTTA TGGTGCAGCC
  421 CAATCCAGAA GCCAGGCTAC GTCGGGCAAA GCCAAGGGCC AGTGATGAGC AGACTCCCTT
  481 GGTGAACTGT CATACTCCCC ACAGCAATGT CATCTTACAT GGTATTGATG GTCCAGCTGC
  541 TGTCCTGAAA CCAGACGAAG TTCATGCTCC ATCAGTAAGC TCCTCTGTTG TGGAAGAAGA
  601 TGCTGAAAAC ACCGTGGATA CTGCTTCCAA GCCAGGACTT CAGGAGCGTC TCCAAAAGCA
  661 TGATATCTCT GAAAGTGTGA ACTTCGATGA GGAGACTGAT GGAATATCCC AGTCAGCATG
  721 TTTAGAAAGA CCCAATTCTG CATCAAGCCA GAATTCAACC GATACAGGCA CTTCCGGTTC
  781 TGCTACTGCC GCCCAACCAG CTGATAACCT CCTGNGAGAC ATAGACTACC TGGAGGNNTT
  841 TGTGTATAGT CCTGCCCCTC AAGNNGTCAC AGTAANATGT CNGATAATCC GGGNNAAAAN
  901 GGGAATGGNT CNGGGNNCNC TNNCCNNNCT ACTATANNNN CTNNGNAAAA AGNNNAAAAT
  961 CANNANNANA TTTCTTNNNG NNNNNNNNAN NNNNNNNNAN NNNNNAANNN NNCNNNNNNN
1021 NNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB15194_B3Gkb.seq check
>D04646A5_B3Gk_1_SV40polyA_R2_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNTN
   61 NTCNGGNNNN NNGNNNNGNG TNNNNNNNNN NNNAANNNNN NNANNNNNNN NNNNNNTGNN
  121 NNNNGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC CCGGGCCCGC GGTACCGTCG
  181 ACTCATTCAC ACGCCAGCTT ACTGTCAAAG CTAGAAAGAC CGATGCCAAA GGCCTGTACT
  241 GCACAAAGTG GGTAGTTGTA ATCCAGTGTG AACACGTCAT CTGCCACACG TCCAAACTGC
  301 ATGACTATAT AATCAGGGTC ATTTTTGTGG ACTATCTGGA AGTTCTTCAC AGACGCCTGA
  361 GTGACCCGGC CACGGAAGTT GAGGACATAG GACTGAGTGT CACTGTTCCA GACGGGGGCC
  421 TTGTTGTGCA GCTCAACCAG ATTTTCCATA GTTCTGTTCT GCCACCTTGA GAGCAAACTG
  481 TCATGGTTGT TTTGTGGCTG ATAGGGGATC TGCTTATGAT TCAGTGTCAT TCCAGGAATG
  541 ATCACAGACA TTTTCCTAGG ACCTTTAAAT CCAAGTACGT TTGTTTCATA GGAGATGGCA
  601 GCCAGCTCCT GCCGGGTGTG GGCCGCTCCT ACCAAACCCC GGCCCTTCAT GGGGCAGATG
  661 CCACGGTCAT AAACTGTAAA CTTGGTCCCC ATGAGGTTGG ATCTAAGCTT GCCGACATAA
  721 CTTTCTCCTT CACGAGNTAA ATCAACTGGA TCAATGGAGA TAAGGTAGTT GGCTGTTTTG
  781 CTCTTTTTCC GCTTTCTAGC TGCAAGAANN NNTATCTTCT GATTTTCTTC TTTTTCCNAG
  841 TACATATAGT ANGTGGGGAA NAGANCCCGA TNCNTTCCCN NTTTANCCCN GNANTATCCG
901 ACATCTNACT GNGNCACNNN NAGGGGCNNN ANTNTACNNN AAGNNNNNNN NNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB15194_B3Gkc.seq check
>D04646A5_B3Gk_1_CMV_Forward_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN
  721 CACCCAGTCC GCCCTGANCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  781 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAN CTGTACAANN NCGGANTCAG
  841 ATCTATGGAN GCTTCGCGCN NCCNGNTCAN NNCCAGCGGN NANNNNNNCT TCCNTGNNNA
901 AANGATGAAN ANNCNNNNNN NTNNNNNNNN NTNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR_KanR
Sequence file: RDB15194_B3Gkd.seq check
>D04646A5_B3Gk_1_SV40pro_ori_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCC GCCTCNNCCT CTGNNCTNTT CCNGAAGTAG TGAGGANGCT
   61 TTTTTGNNNN NNNNNNNTTT TGCAAAGNTC GATCAAGAGA CAGGATGAGG ANNNTTTCGC
  121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
  181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
  241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
  301 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
  361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
  421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
  481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
  541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
  601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC
  661 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
  721 GGCCGCTTTT CTGGATTCAT CGACTGTGNC CGGCTGGGTG TGGCGGANCG CTATCANGAC
  781 ATAGCGTTGG CTACNCGTGA TATTGCTGAA GANCTTGGCG GCGAATGGGC TGACCGCTTC
  841 CTCGNGCTTT ACNGNATCGC CGCTCCCGAN TCGCAGCGCA NCGCNNTCTA TCGCCNTCTT
901 GANNANTNNN TCNNANCGGN NNNNNGGGGN NTCNAAATGN NNNANCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]