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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmCherry-C1-TULP3 (#RDB15195)

Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal mCherry, CMV promoter.

Clone info. Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal mCherry, CMV promoter.
Comment Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAK069G14 (HGX027758).
Vector backbone pmCherry-C1 (modified from pEGFP-C1) (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name human TULP3 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15195 pmCherry-C1-TULP3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmCherry-C1-TULP3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15195).

Reference section:

Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15195_B3Gkp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F (Pr0453)
Region: mCherry 3',insert 5'
Sequence file: RDB15195_B3Gke.seq check
>D04646A6_B3Gk_1_mCherry-C_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNG CCNNNNNCCT ACNACGTNNA CATCAAGTTG GACATCACCT
   61 CCCACAACGN NNNNNNNNNN NTCNNGGAAC AGTACGAACG CGCCGAGGGC CGNNNCTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGGAGGCT TCGCGCTGCC
  181 GGCTCAGTCC CAGCGGCGAC AGTGTCTTCC ATGAAGAAAT GATGAAGATG CGACAGGCTA
  241 AGCTGGATTA TCAGAGGCTA CTACTTGAGA AGAGGCAAAG GAAAAAGCGC CTTGAGCCAT
  301 TTATGGTGCA GCCCAATCCA GAAGCCAGGC TACGTCGGGC AAAGCCAAGG GCCAGTGATG
  361 AGCAGACTCC CTTGGTGAAC TGTCATACTC CCCACAGCAA TGTCATCTTA CATGGTATTG
  421 ATGGTCCAGC TGCTGTCCTG AAACCAGACG AAGTTCATGC TCCATCAGTA AGCTCCTCTG
  481 TTGTGGAAGA AGATGCTGAA AACACCGTGG ATACTGCTTC CAAGCCAGGA CTTCAGGAGC
  541 GTCTCCAAAA GCATGATATC TCTGAAAGTG TGAACTTCGA TGAGGAGACT GATGGAATAT
  601 CCCAGTCAGC ATGTTTAGAA AGACCCAATT CTGCATCAAG CCAGAATTCA ACCGATACAG
  661 GCACTTCCGG TTCTGCTACT GCCGCCCAAC CAGCTGATAA CCTCCTGGGA GACATAGACT
  721 ACCTGGAGGA NTTTGTGTAT AGTCCTGCCC CTCAAGGTGT CACAGTAAGA TGTCGGATAA
  781 TCCGGGATAA AAGGGGAATG GATCGNGGTC TCTTCCCNAC CTACTATATG TACTTGGAAA
  841 AAGAAGAAAA TCAGAAGATA TTTCNTCTTG CAGCTAGAAA GNNGAAAAAG ANCAAAACAG
  901 CCNANTANCT TATCTNCNNN NNTCCNNNNN ANTTATNNCN NGNANNNNNA AAGNTNNGNN
961 NNNNNNNN
//
Primer: SV40LPA-F2 (Pr1235)
Region: insert 3'
Sequence file: RDB15195_B3Gkf.seq check
>D04646A6_B3Gk_1_SV40LPA-F2_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTN NCANCNACNA TTGCATTCAT TTTATGTTTC
   61 NGGTTCAGGG GGNGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
  121 TGGCTGATTA TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACT
  181 CATTCACACG CCAGCTTACT GTCAAAGCTA GAAAGACCGA TGCCAAAGGC CTGTACTGCA
  241 CAAAGTGGGT AGTTGTAATC CAGTGTGAAC ACGTCATCTG CCACACGTCC AAACTGCATG
  301 ACTATATAAT CAGGGTCATT TTTGTGGACT ATCTGGAAGT TCTTCACAGA CGCCTGAGTG
  361 ACCCGGCCAC GGAAGTTGAG GACATAGGAC TGAGTGTCAC TGTTCCAGAC GGGGGCCTTG
  421 TTGTGCAGCT CAACCAGATT TTCCATAGTT CTGTTCTGCC ACCTTGAGAG CAAACTGTCA
  481 TGGTTGTTTT GTGGCTGATA GGGGATCTGC TTATGATTCA GTGTCATTCC AGGAATGATC
  541 ACAGACATTT TCCTAGGACC TTTAAATCCA AGTACGTTTG TTTCATAGGA GATGGCAGCC
  601 AGCTCCTGCC GGGTGTGGGC CGCTCCTACC AAACCCCGGC CCTTCATGGG GCAGATGCCA
  661 CGGTCATAAA CTGTAAACTT GGTCCCCATG ANGTTGGATC TAAGCTTGCC GACATAACTT
  721 TCTCCTTCAC GANATAAATC AACTGGATCA ATGGAGATAA NGTAGTTGGC TGTTTTGCTC
  781 TTTTTCCGCT TTCTANCTGC AAGAANAANT ATCTTCTGAN TTTCTTCTTT TTTNCAAGTN
  841 CATATAGTNN NNGGGGGAAN ANANCCCNNN NCCNNTNNCC NNTTTNANCC CNGANNNNNN
901 NNNN
//
Primer: CMV_Forward (Pr0016)
Region: mCherry 5'
Sequence file: RDB15195_B3Gkg.seq check
>D04646A6_B3Gk_1_CMV_Forward_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGCGGTANG CGTGTNCGGT GGGAGGTCTA
   61 TATAAGCAGA GCTGGNNTAG TGAACCGTCA GATCCGCTAG CGCCACCATG GTGAGCAAGG
  121 GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG
  181 GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG
  241 GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA
  301 TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC
  361 CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG
  421 AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT
  481 ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA
  541 CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG
  601 AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA
  661 CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG
  721 ACATCACCTC CCACAACGAG GNNTACACCA TCGTGNAACA GTACGAACGC GCCGAGGGCC
  781 GCCACTCCAC CGGCGGCATG GACGANCTGT ACAAGTNCGG ANTCANATCT ATGGAGGCTT
  841 CGCGCTGCCN GCTCAGNCCC AGCGGCGANN GTGTCTTCCN TGAANAAANG ATGNNNATGC
901 NACNNGNTNN NCTNGNATTA TCANNNNNNA CTANNNNNNN NNANNNNANN NNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR_KanR
Sequence file: RDB15195_B3Gkh.seq check
>D04646A6_B3Gk_1_SV40pro_ori_F_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCCTCNG CCTCNGNNCT NNTCNNGAAG TAGTGAGGAG
   61 GCTTTTTTGG ANNCNNNNNN TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATNNTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  661 GACGGCGANG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGNNN
  721 NNGNCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCANG
  781 ACATANCGTT GGCTANCCGN GANATTGCTG AANANCTTGG CGGCGAATGG GCTGNNCGCT
  841 TCCTCNNGCT TTANGGNATC GCCGCTCCCN ATTNNCANCG NATCNCCNTC NATCNNNNNN
  901 NNGNNNANNN CNTNNNNANC NGNNNTNNNG GNNNTNNNNA NNNNNCNNNN NNANNNNNNN
961 NCCNNNNNNN NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP]