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Yeast mutant CYP51F1m/pBKS-

Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.

Catalog number RDB16757
Resource name Yeast mutant CYP51F1m/pBKS-
Clone info. Cloning vector of G310D variant of S. cerevisiae cytochrome P450 family 51 subfamily F member 1 (CYP51F1), modification of N-terminal region.
Comment Modified to ATGGCTCTCT TATTAGCAGT TTTT from ATGTCTGCTA CCAAGTCAAT CGTT of nucleic acid sequence coding N-terminal 10 amino acid residues.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae mutated CYP51F1(erg11) cDNA
Depositor|Developer Aoyama, Yuri |
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External Database
Saccharomyces cerevisiae mutated CYP51F1(erg11)
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ishida, N et al. Biochem. Biophys. Res. Commun. 155(1), 317-23, 1988) is requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Ishida, N et al. Biochem. Biophys. Res. Commun. 155(1), 317-23, 1988）の引用を必要とする。
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          RDB16757 Yeast mutant CYP51F1m/pBKS- DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ishida, N., A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS. Biochem. Biophys. Res. Commun. 155 (1): 317-323 (1988). PMID 3046615.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16757_A8K1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Region: insert 5'
          Sequence file: RDB16757_A8K1a.seq check
          >D05837A5_A8K1_3_M13_-40_F02_17_ABI24.ab1
              1 AAGTCAGCGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CACCGCGGTG
             61 GCGGCCGCTC TAGAACTAGT GGATCCATCG ATGCTTAGGA GGTCATATGG CTCTGTTATT
            121 AGCAGTTTTT GGAGAGGCAT TGGAATACGT AAACATTGGT TTAAGTCATT TCTTGGCTTT
            181 ACCATTGGCC CAAAGAATCT CTTTGATCAT AATAATTCCT TTCATTTACA ATATTGTATG
            241 GCAATTACTA TATTCTTTGA GAAAGGACCG TCCACCTCTA GTGTTTTACT GGATTCCATG
            301 GGTCGGTAGT GCTGTTGTGT ACGGTATGAA GCCATACGAG TTTTTCGAAG AATGTCAAAA
            361 GAAATACGGT GATATTTTTT CATTCGTTTT GTTAGGAAGA GTCATGACTG TGTATTTAGG
            421 ACCAAAGGGT CACGAATTTG TCTTCAACGC TAAGTTGGCA GATGTTTCAG CAGAAGCTGC
            481 TTACGCTCAT TTGACTACTC CAGTTTTCGG TAAAGGTGTT ATTTACGATT GTCCAAATTC
            541 TAGATTGATG GAGCAAAAGA AGTTTGTTAA GGGTGCTCTA ACCAAAGAAG CCTTCAAGAG
            601 CTACGTTCCA TTGATTGCTG AAGAAGTGTA CAAGTACTTC AGAGACTCCA AAAACTTCCG
            661 TTTGAATGAA AGAACTACTG GTACTATTGA CGTGATGGTT ACTCAACCTG AAATGACTAT
            721 TTTCACCGCT TCAAGATCAT TATTGGGTAA GGAAATGAGA GCAAAATTGG ATACCGATTT
            781 TGCTTACTTG TACAGTGATT TGGATAAGGG TTTCACTCCA ATCAACTTCG TCTTCCCTAA
            841 CTTACCATTG GAACACTATA GAAAGAGAGA TCACGCTCAA AAGGCTATCT CCGGTACTTA
            901 CATGTCTTTG ATTAAGGAAA GAAGAAAAGA ACAACGACAT TCAAGACAGA GATTTGATCG
            961 ATTCCTTGAT GAAGAACTCT ACCTACAAGG ATGGTGTGAA GATGACTGAT CAAGAATCGC
           1021 TAACTTGTTA ATTGATGTCT TAATGGGTGG TCAACATACT TCTGCTGCCA CTTCTGCTTG
           1081 GATTTTGTTG CACTTGCTGA AAGACCAGAT GTTCCAACAA GGATGTACGA GAACAATGCT
          1141 GTTTTGGATG TTGTAGAAGG ATGACTACGA TTATACAGGA AATGGTGAC
          //
          Primer: Reverse2
          Region: insert 3'
          Sequence file: RDB16757_A8K1b.seq check
          >D05837A5_A8K1_3_reverse2_G02_20_ABI24.ab1
              1 AAGGACAATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
             61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTT TAGATCTTTT GTTCTGGATT
            121 TCTCTTTTCC CAGATGATCT TGGCTGGACC GGTTGGAAGA GTAACCATAG ATGTAAAGTC
            181 AGGAGGTGGA ACGGTCTTAC CCTCTGGGTA ATGCCATTTT AATGTTCTGA TAAAAATGGA
            241 CATTAGAACA CCTAGCTGAC AGTAAGCAAA GTGTTCACCG ATACATCTGT GTCTACCACC
            301 ACCGAAAGGT AAGTATGGAG AGCTGACACC CTTAGAAATG GCACCGAAAC CGTAATCGAC
            361 TTCTTCACCG ACGGAATAAG AGGAGGCAGA ATCTTTGTTC CAACGGTGAA TGTTGAATTG
            421 GTGAGCATTA GGGAAGTATT CGTCTCTTAA ATGAGTGTAA CCTGGAGAAA CCAAAACGTG
            481 ATAACCTGCT GGGATGACAT AAGAAGTGTT TGGAACGTGC ATATCTTTCA TAACCTTACG
            541 GAACAAAGAG TGCAATGGAT GGTGCATTCT TAGAGTTTCC TTAATAGTTT GGTTCAACAA
            601 TGGCATTTCT TGTAATAAAT CGTAGGTCAA TTCCTTCTTA CCACCATCCA AAACACGCAT
            661 TTGTTCTTCG TACAATTCTT GTTGGACATC TGGTCTTTCA GCCAAGTGCA ACAAAATCCA
            721 AGCAGAAGTG GCAGCAGAAG TATGTTGACC ACCCATTAAG ACATCAATTA ACAAGTTAGC
            781 GATTTCTTGA TCAGTCATCT TCACACCATC CTTGTAGGTA GAGTTCTTCA TCAAGGAATC
            841 GATCAAATCT CTGTCTTGAA TGTCGTTGTT CTTTCTTCTT TCCTTAATCA AAGACATGTA
            901 AGTACCGGAG ATAGCCTTTT GAGCGTGATC TCTCTTTCTA TAGTGTTCCA ATGGTAAGTT
            961 AGGGAAGACG AAGTTGATTG GAGTGAAACC CTTATCCAAA TCACTGTACA AGTAAGCAAA
           1021 ATCGGTATCC AATTTTGCTC TCATTTCCTT TACCCAATAA TGATCTGAGC GGTGAAAATA
           1081 GTCATTTCAG CTGAGTACAT CACGTCATAG TTACCAGTAG TCTTTCATCC AACAAAGTTT
          1141 TGAGTCTCTT GAAGTACTGA ACCGTCTCAG CATTCATGTG TGAC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Ishida, N., A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS. Biochem. Biophys. Res. Commun. 155 (1): 317-323 (1988). PMID 3046615.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl