Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-KIF13A-tail-deltaRHD (#RDB17232)

Expression vector of mouse KIF13A fused with EGFP. The KIF13A-tail mutant without a motor domain and the lacks an RHD, a Rab10-binding dificient mutant.

Clone info.	Expression vector of mouse kinesin family member 13A (KIF13A) fused with EGFP at N-terminus. The KIF13A-tail mutant without a motor domain and the lacks an Rab10-binding homology domain (RHD), a Rab10-binding dificient mutant, see Fig. 4A and 5C of Etoh, K. and Fukuda, M., J. Cell Sci., 132 (5), jcs226977, 2019.
Comment	Expression was confirmed by the depositor with immunoblotting.
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name	mouse Kif13a cDNA
Depositor|Developer	Fukuda, Mitsunori |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17232	pEGFP-C1-KIF13A-tail-deltaRHD	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

---

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-KIF13A-tail-deltaRHD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17232).

Reference section:

Etoh, K., Fukuda, M., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

---

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17232_A9Elp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3', insert 5' Sequence file: RDB17232_A9Ela.seq
>D06280B5_A9El_1_EGFP-C2_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNCGC NNNCNCNTGN NCCTGCTGGN NTTCGTGNNC GCCGCCGGGN    61 TCNCTCTCGG CATGGNCGAN CTGTACAAGT CCGGACTCAG ATCCGACCCC AATGCCAAGG   121 TCATCCGAGA GCTTCGAGAG GAAGTGGAGA AACTGAGGGA GCAGCTCTCG CAGGCTGAGG   181 CCATGAAGGC CCCCGAGCTA AAGGAGAAGC TCGAAGAGTC TGAGAAGCTG ATAAAGGAGC   241 TAACGGTGAC GTGGGAAGAG AAGCTGAGGA AAACAGAAGC GATCGCACAG GAGAGACAGC   301 GGCAGCTGGA AAGCATGGGA ATCTCCCTGG AGACCTCTGG CATCAAGGTG GGGGACGACA   361 AGTGCTACCT CGTCAACCTG AACGCAGACC CTGCCCTCAA CGAGCTTCTG GTTTACTATT   421 TAAAGGATCA TACCCGGGTG GGTGCAGATA CCTCTCAGGA CATCCAGCTC TTCGGAATAG   481 GGATTCAGCC CGAGCACTGT GAGATCGACA TCGCAGCGGA TGGAGACATC ACTCTCACGC   541 CCAAAGAAAA TGCAAGGTCC TGTGTCAATG GCACCCTTGT CTGCAGTACT ACCCAGCTGT   601 GGCACGGCGA CAGGATCTTA TGGGGAAACA ACCACTTTTT TAGAATTAAC TTGCCTAAGA   661 GGAAACGGCG GGACTGGTTG AAAGACTTTG AGAGAGAAAN CAGTTCCGCG GAGCACGATC   721 TAGACGCTGC GAGCGANGCT TCCTCGGANC CNGACTACNA CTACGANTTC GCACAGATGG   781 ANGTCATCAT GAAAACACNG AACAGCANTG NTNCAGTTCA AAANGTGGTT CANGTCCNNN 841 NNAANCANTA CCNNNNNNNN NN //
Primer: SV40poltA_R2 (Pr0623) Region: insert 3' Sequence file: RDB17232_A9Elb.seq
>D06280B5_A9El_1_SV40polyA_R2_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNGNNN NNNACAAGTT AACAACAACA ATTGNATTCA TTTTATGTTT    61 CAGGTTCAGG GGGNGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT   121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC   181 CTGCAGGCGG CCGCGGGAAT TCGATTTTAC CGAGTGGCTG TGGCTTCCTG GTCGTTCTCT   241 ATCTTGGGAT GCAGCCGGTC GGCATCTGGA GGTCTTTCTG GGATACCTTT ATGGTCTGTT   301 TCGTTTGTTA GAGCCCTCCT CCTGGAGGGC TCCCCTACAG AACAATCTGC CAAACCATCA   361 AAGTCTTTGC CATCAGAAAC ACCCATGAAC TCTGTGAAGG AATGGTCCTC CAAAATGTTT   421 GTAGTGTGTT CTATCGTCAC CTCTCCTGGG CAGAATTCTC TGGCTTGGTG GCCATCTTTG   481 CTGGGGCCGA CATCCAGTTC TGAACATGAA GCAGTCCTGC ATGGCATTCT GGAACTTTCC   541 TCAGGGATGC TCTGGGGTGA CGGGGTCCCC TTGGGTAAGG CACTGTTTTC CTCCAGCGGC   601 ACAGCGATTG TCTCATCCTT CCCTGAGCCT CTTCCTACTT CTGTAAGCTC CTGATTCGAA   661 GCTGGTCTGA CATCCGGTGC AAGTGATGGT CCCGGGGGCT CGGCGCACTC GACCTCCTTT   721 GTCGAGACGG CAAGCTGGTC TGANCTGTCG CTNGNNGGGA NTGCCATGTC AGACAGAGTG   781 NCATTGGANG CACTGNNGGA NAAGTANCCA CTNGNGNNGC TGCTGGNNGN ANGGCNACGG   841 GNNANNNCTT NNTNNNNNNN TNTNGANTCT GNCNNNTCCN CCTTANAANN ANGNNNNNTC 901 CNNNNTNNTN CNANNNNNNN NNN //
Primer: mKIF12A_F1 (Pr0883) Region: insert mid Sequence file: RDB17232_A9Elc.seq
>D06280B5_A9El_1_mKIF13A_F14_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TGGCTGCGTG NNCGCCNGGT CCNCCAAGCT GCAGAGAGGG    61 CTGGACAGCT ACCAGAGAGA TGAGGAGGAT GGCGGTGATA TGGATAGTTA TCAGGAAGAA   121 GACTTGAATT GTGTAAGAGA GAGATGGTCC GATGCAGTGC TGGTGCCCGC CCCTGGCAGC   181 GGAATCCCCG GGGCACCTGC TGACTGGGTC CCACCTCCTG GCATGGAAAC ACACATCCCG   241 GTTCTCTTCC TGGATCTGAA CGCGGATGAC CTCAGTGCCA ATGAGCAGCT GGTGGGCCCC   301 CATGCGTCCG GTGTGAACTC CATCCTGCCC AAGGAACACG GCAGTCAGTT TTTCTACCTG   361 CCCATCATAA AGCACAGTGA TGACGAGGTT TCAGCCACAG CCTCCTGGGA CTCCTCTGTG   421 CACGACTCTC TTCACCTGAA TCGGGTCACA CCGCAGAATG AAAGGATCTA CCTGATCGTG   481 AAGACCACCG TGCAGCTCAG CCACCCGGCC GCCATGGAGC TAGTGTTGCG GAAGCGGATC   541 GCGGCCAATA TTTATAACAA GCAGAGTTTC ACCCAGAGTT TGAAGAGACG AATATCACTG   601 ATCAATATAT GTTACTCCTG CGGCGTGACC TATGAGATAG TATCCAATAT CCCAAAGGCG   661 ACCGAGGAGA TTGAGGANCG GGAAACCCTG GCCCTCCTGG CAGCGCGGAG TGAGAACGAG   721 GGCACTTTGG ATGGGGAGAC ATACATCGAG AAGTACACGC GGGGCGTGCT GCAGGTGGAG   781 AATATCCTGA NCCTGGANCG GCTCCGGCAN GCTGTCACGG TGAANGAAGC GCTTTCCNCC   841 AAAGCGAGGC ACATTCNGAN AANCCTCAGT ACANCGAACG TTCACNACGT CNCCTCTANC   901 CNGNCNGATN NCTCNGGNTT CGATGAAGAN NANNNNNNCT GGNCNNANAN NNANNNANAN 961 NNGTCTGANN NCNGCTNCNN CTNNNNNGNN NNCNNNNNNN NNN //
Primer: CMV_Forward (Pr00016) Region: CMV pro, EGFP 5' Sequence file: RDB17232_A9Eld.seq
>D06280B5_A9El_1_CMV_Forward_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC   121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC   361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG   601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC   661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC   721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG   781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGNCGANC TGTACAAGTC CGGANTCAGA   841 TCCGACCCCN ATGCCNAGGN CATCCGAGAN CTTCNAGAGG AAGTGGAGAA ANNNANGGAN   901 CAGCTCTCGC ANGNNNANGC CNTGNANNNN CCCCNNNNCN NAAGGANANN CNCGAANANN 961 NTGNNNANNC TGANAAANGN ANCTNNNNGN NNNNNNNGGN ANNANNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB17232_A9Ele.seq
>D06280B5_A9El_1_SV40pro_ori_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG CCNCNGCCTC TGNNCTATTC NNGAAGTAGT GAGGANGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA   121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG   181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG   241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC   301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC   361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG   421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC   481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA   541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG   601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG   661 GCGANGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG   721 GCCGCTTTTC TGGATTCATC GACNGNGGCC GGCTGGGNGT GGCGGANCGC TATCANGACA   781 TAGCGTTGGC TACCCGNNAT ATNGCTGNNN NNCTNGGCNG CNAANGGGNN GANNGCTNNC   841 TCNNNCTTNA NNGNATCGCC GCNCCNNATT CNCNNCNNNN CGCNNTNNNN NCNNCNNNNN 901 NNNNN //

Please visit Sequencing and PCR primers for primer information.

---

References

Original, user report and related articles

original	Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]
reference	Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263. [link to RRC of NBRP]

Resource search

Go!

Link

Other resources in our bank

• mouse Kif13a

---

External Database
mouse Kif13a

• in KEGG Gene
• in KEGG Orthology
• in NCBI Gene

---

Reference sequence
  

Tips

Featured content

---