Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-Myc-KIF13A (#RDB17234)

Retroviral expression vector of mouse KIF13A fused with Myc.

Clone info. Retroviral expression vector of mouse kinesin family member 13A (KIF13A) fused with Myc at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pMRX-IRES-bsr (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cell).
Gene/insert name mouse Kif13a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17234 pMRX-bsr-Myc-KIF13A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-Myc-KIF13A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17234).

Reference section:

Etoh, K., Fukuda, M., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17234_B0Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region, Myc, insert 5'
Sequence file: RDB17234_B0Fma.seq check
>D06280B7_B0Fm_3_MMLV-pol_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGGCN TCGCANCTTN GNNACACGCC GCCCACGTGA AGGCTGCCGA
   61 CCCCGGGGGT GGNCCATCCT CTAGACTGCC GGATCAGCTT GGCATGGAGC AAAAGCTCAT
  121 TTCTGAAGAG GACTTGACGC GTGATTATGA TATTCCAACT ACTGCTAGCG AGAATTTGTA
  181 TTTTCAGGGT GAGCTCGGAT CCATGTCGGA TACGAAGGTA AAAGTTGCCG TCCGGGTCCG
  241 GCCCATGAAC CGAAGAGAGC TGGAACTGAA CACCAAGTGC GTGGTGGAGA TGGAAGGGAA
  301 CCAGACGGTC CTGCACCCTC CTCCTTCCAA CACTAAGCAG GGAGAAAGAA AACCTCCCAA
  361 GGTGTTTGCC TTTGATTATT GCTTTTGGTC CATGGATGAA TCGAACACAA CAAAATATGC
  421 TGGTCAAGAA GTCGTTTTCA AGTGCCTCGG GGAAGGAATT CTCGAGAAAG CCTTTCAGGG
  481 ATACAATGCT TGTATTTTTG CATATGGCCA GACAGGTTCT GGAAAGTCCT TTTCCATGAT
  541 GGGCCACGCA GAGCAGCTGG GCCTTATCCC AAGGCTCTGC TGTGCTCTGT TTCAAAGGAT
  601 CGCCCTGGAG CAAAATGAGT CACAGACCTT CAAAGTCGAG GTATCGTATA TGGAGATCTA
  661 TAACGAGAAG GTCCGGGACC TCTTGGANCC CAAAGGGAGC ANGCAGTCTC TGAAAGTCCG
  721 ANAGCATAAN GTCCTGGGGG CCGTATGTCG ATGGTCTGTC TCAGCTGGCT GTCACCAGCT
  781 TTGAGGANAT TGNNTCACTG ATGTCTGANG GAAATAAGTC TCGAACGGNA NCAGCGNNCA
  841 ACATGANTGA NGANAGCAGC CGTTCCCATG CNNNCTTCAA CATCNTCATC ACACAGANNC
  901 NGNANNNNCN ACANNNNGGG NANNTNCNNG GGNNNNANNN TCANCNNNGN NNNNNCNNNG
961 NNNNNNNNNN
//
Primer: IRES-R (Pr0037)
Region: insert 3'
Sequence file: RDB17234_B0Fmb.seq check
>D06280B7_B0Fm_3_IRES-R_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGN CNNNNNNNNN NGGGGGGGGG GGCGGNATTG ANCCCGCTAG
   61 AGGGAAACCG TTGCTNNNNN NNNNTAGTTA ACAATTGTTA CCGCGGCCGC GGGAATTCGA
  121 TTTTACCGAG TGGCTGTGGC TTCCTGGTCG TTCTCTATCT TGGGATGCAG CCGGTCGGCA
  181 TCTGGAGGTC TTTCTGGGAT ACCTTTATGG TCTGTTTCGT TTGTTAGAGC CCTCCTCCTG
  241 GAGGGCTCCC CTACAGAACA ATCTGCCAAA CCATCAAAGT CTTTGCCATC AGAAACACCC
  301 ATGAACTCTG TGAAGGAATG GTCCTCCAAA ATGTTTGTAG TGTGTTCTAT CGTCACCTCT
  361 CCTGGGCAGA ATTCTCTGGC TTGGTGGCCA TCTTTGCTGG GGCCGACATC CAGTTCTGAA
  421 CATGAAGCAG TCCTGCATGG CATTCTGGAA CTTTCCTCAG GGATGCTCTG GGGTGACGGG
  481 GTCCCCTTGG GTAAGGCACT GTTTTCCTCC AGCGGCACAG CGATTGTCTC ATCCTTCCCT
  541 GAGCCTCTTC CTACTTCTGT AAGCTCCTGA TTCGAAGCTG GTCTGACATC CGGTGCAAGT
  601 GATGGTCCCG GGGGCTCGGC GCACTCGACC TCCTTTGTCG AGACGGCAAG CTGGTCTGAG
  661 CTGTCNCTGG ANGGGACTGC CATGTCAGAC AGAGTGGCAT TGGAGGCNCT GTGGGANAAG
  721 TAGCCACTGG TGAGGCTGCT GGTGGTAGGG CTACGGGACA CTGCTTTCTC CAAGGCTCTC
  781 GANNCTGTCA GGTCCGCCTT ANAAGANGNN CACTCCNNNT TCTCCAAGCT GGCATTGTAA
  841 ACNCTNAANT CNGCNNNNNN CNNNGGGCAN NNNANGNTGT GNANGCATCA GNNNNCNTNN
901 TNANNNCNNN CNNNNNCNTT TTNNNNCNNN NNNNNNN
//
Primer: MMLV psi_F (Pr0863)
Region: MMLV psi, gag
Sequence file: RDB17234_B0Fmc.seq check
>D06280B7_B0Fm_3_MMLVpsi_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNCNNNNNN NTGNNNTTTT GCTTTCGGTT TGGGNNCGAA GCCGCGCCGC
   61 GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT
  121 GTCTGAAAAT ATGGGCCCGG GCCAGACTGT TACCACTCCC TTAAGTTTGA CCTTAGGTCA
  181 CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA GATGTCAAGA AGAGACGTTG
  241 GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC GGATGGCCGC GAGACGGCAC
  301 CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG
  361 ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC
  421 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT CCGCCCCGTC
  481 TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC TCCCTTTATC CAGCCCTCAC
  541 TCCTTCTCTA GGCGCCCCCA TATGGCCATA TGAGATCTTA TATGGGGCAC CCCCGCCCCT
  601 TGTAAACTTC CCTGACCCTG ACATGACAAG AGTTACTAAC AGCCCCTCTC TCCAAGCTCA
  661 CTTACAGGCT CTCTACTTAG TCCAGCACGA AGTCTGGAGA CCTCTGGCGG CAGCCTANCA
  721 AGANCNACTG GANCGACCGG TGGTACCTCA CCCTTACCGA GTCGGCGACA CAGTGTGGGT
  781 CCGCCGANAC NAGACTAAGA NNCTANAACC TCGCTGGAAN NGANCTTACA CAGNNCNGCT
  841 GNNCACCCCC NACNGNCCNT CAANNNANAC NGNATCNCAN NTNGGNATAC NCGCCNNCNA
  901 CNNNNAANGG NNNNCNGAAN CCCCNGGGGG NNGNNNNNNC CNNNNNNACN NNCNNGNNNN
961 ANNNNTNNNN NNNNNN
//
Primer: Amp_R (Pr0008)
Region: Amp pro, CMV en, delta U3 5'LTR
Sequence file: RDB17234_B0Fmd.seq check
>D06280B7_B0Fm_3_Amp_R_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNGGGTTA TTGTCTCNNT GNGCGGANAC
   61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
  181 ATCACGAGGC CCTTTCGTCT TCACTCGAGG AGCTTGGCCC ATTGCATACG TTGTATCCAT
  241 ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT ACCGCCATGT TGACATTGAT
  301 TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG
  361 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
  421 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
  481 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
  541 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG
  601 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG
  661 CTATTACCAT GCTAGAGAAC CATCAGATGT TTCCNGGGTG CCCCAAGGAC CTGAAANTGA
  721 CCCTGTGCCT TATTTGAACN NNCCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT
  781 GCTCCCCGAG CTCAATAAAA GANNCNACAA CCCCTCACTC GGCGCGCCAG TCCTCCGATT
  841 GACTGANTCG CCCGGNTANC CNNGTATCCA ATAAANCCCT CNNNNANTTG CATCCNANNT
  901 NNNGGTCNNN CNNNTNCCNT NNGNANGNTC TCCNCTGANN NNNTGANNNN CCNNNNNCNN
961 NNNNTTNNNN TNNNNGGNNT NNNCNNNNNN NNNN
//
Primer: IRES-F (Pr0036)
Region: IRES, bsr, 3' LTR
Sequence file: RDB17234_B0Fme.seq check
>D06280B7_B0Fm_3_IRES-F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCGNNGT TAANAAAACG TCTAGGCCCC CCGAACCACG
   61 GGGNNGTGGT TTTCCTTTGN AAAACACGAT AATACCATGG TCATGAAAAC ATTTAACATT
  121 TCTCAACAAG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG
  181 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA
  241 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT
  301 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT
  361 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG
  421 ATTTCAGACT ATGCACCAGA TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA
  481 ACTACGATTG AAGAACTCAT TCCACTCAAA TATACCCGAA ATTAAAAGTC GACGATAAAA
  541 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT
  601 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA
  661 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA
  721 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NNGCCAAGAA CAGATGGAAC AGCTGANTAT
  781 GGGCNNAACN NGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCNNGGCC NNGAACAGAT
  841 GGTCCCCANA TGCGGNCNAG CCCTCAGCAN TTNCTANANA NNCATCANAT GNTTTCCNNG
  901 GTGNCCCNAN GGANCNNAAN NGANCCCTNN NNCNTNATTT NNANNNNANN NNANTCANNN
961 CGCNTNNNNN NNTNNNNNNN NNGNNNNTNN NNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]