Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-puro-HA-KIF13B (#RDB17235)

Retroviral expression vector of mouse KIF13B fused with HA.

Clone info. Retroviral expression vector of mouse kinesin family member 13B (KIF13B) fused with HA at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pMRX-IRES-puro (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cell).
Gene/insert name mouse Kif13b cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17235 pMRX-puro-HA-KIF13B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-puro-HA-KIF13B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17235).

Reference section:

Etoh, K., Fukuda, M., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17235_B0Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol, HA, insert 5'
Sequence file: RDB17235_B0Fma.seq check
>D6280B8_B0Fm_2_MMLV-pro_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGGN ATCGCANCTT GGNNACNCGC CGCCCNCGTG AAGGCTGCCG
   61 ACCCCGGGGG TGGANNNTCC TCTAGACTGC CGGATCTCCA CCATGGGTTA CCCATACGAC
  121 GTCCCAGACT ACGCTGGATC CATGGGAGAC TCCAAAGTGA AAGTGGCGGT ACGGGTCCGG
  181 CCAATGAACC GACGAGAAAT TGACTTACAT ACCAAATGTG TGGTGGATGT TGAAGCGAAC
  241 AAAGTTATTC TGAATCCTGT AAATACAAAT CTTTCCAAAG GAGATGCCCG GGGCCAGCCA
  301 AAGATCTTTG CTTATGATCA CTGTTTCTGG TCCATGGATG AATCTGTCCG AGAGAAGTAT
  361 GCAGGTCAAG AAGATGTTTT CAAGTGCCTT GGGGAGAACA TTCTTCAGAA TGCATTTGAT
  421 GGCTACAACG CCTGTATCTT TGCTTATGGG CAGACAGGGT CTGGAAAGTC TTACACCATG
  481 ATGGGCACTG CTGACCAACC TGGATTAATC CCAAGGCTCT GTAGTGGACT CTTTGAACGA
  541 ACCCAGAAAG AGGAAAATGA AGAACAGAGT TTTAAAGTCG AAGTGTCGTA CATGGAAATT
  601 TATAATGAAA AAGTCCGAGA CCTTCTTGAC CCCAAAGGAA GCCGTCAGAC GTTGAAAGTG
  661 AGAGAGCACA GTGTGTTGGG ACCCTACGTG GATGGACTTT CTAAACTGGC TGTCACAAGC
  721 TACAAGGATA TTGAGTCTCT GATGTCTGAN GGCAACAAGT CTCGGACAGT TGCTGCAACC
  781 NACATGAACG ANGAAAGTAG CCGTTCNNNT GCAGNCTTCN AAATCNCCNN GACNCATACT
  841 CTGNATGATG TGAANTCTGG GANATCTGGN NAGAAAGTNG GCAANCNNAN CTTANNNNNN
  901 TNNNCNNNNA NNGANCGANC NNNNAAANNN NNNNCTGCNN NNNANAGGNN NNNNNAGGNN
961 ANCAACNTNN NNNNNNNCNN NNNNNNNN
//
Primer: IRES-R (Pr0037)
Region: insert 3'
Sequence file: RDB17235_B0Fmb.seq check
>D06280B8_B0Fm_2_IRES-R_E06_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAG GGGGGGGGGC GGNNNTTACG TAGCGGCCGC
   61 GAATTCGATT TTAGCTGGCC CAGGACTTCC GGTTCTCAGG ATTCTTGTAA CTCCTGTCTC
  121 CCTTGGCCAG TGCAGCTGTC AGTGAAGCCA GGTTGGTGGC AGAGCCCGAG ATGGTAGCAC
  181 TTCTGCGGAC CTCTGGGGCT CCCTGAGGCT GGAGCCCTGA ACTGCGCCTG CGCCCAGTGC
  241 CCACAGCCCT GCGGACCCGG CTGGGCCTCA CCAGCAGCCC ATAGCCAGGG TTGCACCTGA
  301 AATACTGTTT CCCACCAATG GAGCCATCAT TCTTTCCTGC AGGCAGATCC AGCTCCACAC
  361 CAATCCATGT TCCCTCCTGG AAATCAGTTG GTCCAATATA CCGTACAATG CCCGTCTTGT
  421 TGGTGCCCAC AACAACATAC TCTCCCTCTC TGAGCCACTC AGGGACTTCG CTGGCATCCT
  481 CAGAGTCAGA TGTTACTTCT AGCCTCCCCA GAGTCTGTCC CCTAGCATTG CTGGGGTCTT
  541 CTGAAGCCAG GGGGTCCTCC TCTGCTCCGG ANGAGGCACC ACCCAGCATG CCTGTGAAGG
  601 ACTTAAGCTC TGANGTCCGC ACTTTCCGGA TTCTGAATGG GGACGTGGGT ACAGCAGACG
  661 CGGGTTCAGA AAGAANAGGT GCTCTGGGAA CCTCCAGCTC TGTGCTTTGA GTAGGAGCTG
  721 CAGGTACGTG GGCCTCCTCT GGNGCANGAN GANGGCTTAN TGTGCAGGAN ANGAAAGCCA
  781 GCTCANGCTC TGNGNTGACN TGNCNCANGG CANGGGGGGA NNNANGCGTC NGNCTGNNNA
841 NGNCNGNGGN GNNNAGGCCN NANGNGAGTG TTTCTGANAN GNNNGCAGAN NNNNNNNNN
//
Primer: MMLV psi_F (Pr0863)
Region: MMLV psi, gag, pol
Sequence file: RDB17235_B0Fmc.seq check
>D6280B8_B0Fm_2_MMLV psi_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT GATTTTTGNT TTCGGTTTGG GANCGAAGCC GCGCCGCGCG
   61 TCTTGTCTGC TGCAGCATCG TTCTGTGTTG TCTCTGTCTG ACTGTGTTTC TGTATTTGTC
  121 TGAAAATATG GGCCCGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT TAGGTCACTG
  181 GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA GACGTTGGGT
  241 TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG ACGGCACCTT
  301 TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC CGCATGGACA
  361 CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC CCCCTCCCTG
  421 GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT
  481 CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC
  541 TTCTCTAGGC GCCCCCATAT GGCCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT
  601 AAACTTCCCT GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT
  661 ACAGGCTCTC TACTTAGTCC AGCACGAAGT CTGGAGANCT CTGGCGGCAG CCTACCAAGA
  721 ACAACTGGAC CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG
  781 CCGACACCAG ACTAAGAACC TAGAACNTCG CTNGAAAGGA NCTTACACAG NCCTGCTGAN
  841 CACCCCCACC GCCCTCNAAG TANACGGCAT CGCANCTNNN NNACNCGCCG CCCNCNNNAA
  901 NGNNGNCNNN CCNNGGGGGN NGGANCNNNC NNNNNNNNGN CNNNNNNNCN NCNNNGNNNA
961 CCNNTACNNN NNNCNNNNNN NNNN
//
Primer: IRES-F (Pr0036)
Region: IRES, PuroR
Sequence file: RDB17235_B0Fmd.seq check
>D06280B8_B0Fm_2_IRES-F_F06_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCNNNGTTA ANANAACGTC TAGGCCCCCC GAACCACGGG
   61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA CCGAGTACAA
  121 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC
  181 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG
  241 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG
  301 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG
  361 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC
  421 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT
  481 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT
  541 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC
  601 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC
  661 CGAAGGANCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC CGCCCCACGA
  721 CCCGCAGCGC CCGACCNNNN GANCGCACGA NCCCATGGCT CGACNATAAA ATAAAANANT
  781 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTANGTT TGGCAAGCTA
  841 GCTTAAGTAN CGCNATTTTG CANGGCNTNN NAAAAATACN TNANNTNNNA ANNNAGAANT
  901 TCANATNNAG GNCNNNANCN NATGGNANNN NCNGNNNNTG GGCNAANCNN NNNANCNNNN
961 GNNNANNNNN NNNNCNNNNC CNNGNNNNNN NNNN
//
Primer: PuroR_F (Pr0554)
Region: PuroR, 3' LTR
Sequence file: RDB17235_B0Fme.seq check
>D06280B8_B0Fm_2_PuroR_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NGNNNGANGN NCGCGCACCT GGTGCATGAN CCGCAAGCCC
   61 GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT
  121 GGCTCGACGA TAAAATAAAA GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC
  181 CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT
  241 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT
  301 GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT
  361 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
  421 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT
  481 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC
  541 AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC
  601 CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG
  661 TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG
  721 TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC ATGTATCNAA
  781 ATTAATTTGG TTTTTTTTCT TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGNAT
  841 CGGNCAACGC GCGGGGGANA GGCGGTTTGC GTATTGGGCG CNCTNCCGCT TNCTCGCNCA
  901 CTNANTCGCT GCGNNCGGNC NNNCNGNNGC NGNGANCNGN NATCANCNCA CNCNANGGNG
961 GTANTACNNN NATCNNNNNN ANNNNNNGNN NNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro, CMV en, delU3 5' LTR
Sequence file: RDB17235_B0Fmf.seq check
>D6280B8_B0Fm_2_Amp_R_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTGN NGCANTNATN NGGGTTATTG TCTCNTGAGC GGATACATAT
   61 TTGAATGTAT TTNNNNNAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  181 CGAGGCCCTT TCGTCTTCAC TCGAGGAGCT TGGCCCATTG CATACGTTGT ATCCATATCA
  241 TAATATGTAC ATTTATATTG GCTCATGTCC AACATTACCG CCATGTTGAC ATTGATTATT
  301 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
  361 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
  421 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
  481 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
  541 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
  601 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
  661 TACCATGCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGANCTGA AATGACCCTG
  721 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
  781 CCGANCTCAA TAAAAGAGCC CACAACCCCT CANTCNGCGC GCCAGTCCTC CGATTGACTG
  841 ANTCGCCCGG NNACCCGNGT ATCCNNTAAA CCCTCTNGCA NTTNCATNCN ANTTNNGGNN
  901 NNGCNNTTCC TTGGGNNGNN NNNCNNNNAN NGNNTGNNTA CCNGNCNNCG GGGGNNNTTT
961 CNTTTGGGGG NNNCNNNNNG NNNNNGNNNN NCCNNNNCNN NGGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. [link to RRC of NBRP]