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pGEX-4T-3-Slac2-b-SHD (#RDB17372)

Expression vector of human Slac2-b/KIAA0624 (containing SHD) .

Clone info. Expression vector of human Slac2-b/KIAA0624 (containing the synaptotagmin-like protein homology domain (SHD)) tagged with GST at N-terminus, tac promoter.
Comment A GTP-Rab27 trapper. Also known as EXPH5
Vector backbone pGEX-4T-3 (plasmid)
Selectable markers Amp^r.
Gene/insert name human EXPH5 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB17372hts01.seq checkAssembled from experimentally sequenced data.
Publication Shirakawa, R., J. Biol. Chem. 279 (11): 10730-10737 (2004). [link to RRC of NBRP]
Test sheet RDB17372_B1Elp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17372 pGEX-4T-3-Slac2-b-SHD DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGEX-4T-3-Slac2-b-SHD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17372).

Reference section:

Shirakawa, R., Higashi, T., Tabuchi, A., Yoshioka, A., Nishioka, H., Fukuda, M., Kita, T., Horiuchi, H., Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets. J. Biol. Chem. 279 (11): 10730-10737 (2004). PMID 14699162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shirakawa, R., Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets. J. Biol. Chem. 279 (11): 10730-10737 (2004). PMID 14699162. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]