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pCRTK2x2-ISL1TEBl

Donor vector for knocking-in of tdTomato reporter to ISL1 gene.

Catalog number RDB18669
Resource name pCRTK2x2-ISL1TEBl
Clone info. Donor vector for knocking-in of tdTomato reporter to ISL1 gene. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Comment 2xHSVtk was from RDB01692 pCM-TK
Vector backbone pCR Blunt II (plasmid)
Size of vector backbone 3.5 kb
Depositor|Developer Nakade, Koji |
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External Database
human ISL1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。
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          RDB18669 pCRTK2x2-ISL1TEBl Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCRTK2x2-ISL1TEBl was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18669).

          Reference section:

          Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
          reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep Methods. 2023 Dec 1:100662. doi: 10.1016/j.crmeth.2023.100662. Epub ahead of print PMID 38086384.

          2023.12.14

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