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pCRtk2x2NN

Backbone vector for constructing donor vector with double HSV-thymidine kinase.

Catalog number RDB18670
Resource name pCRtk2x2NN
Clone info. Backbone vector for constructing donor vector with double HSV-thymidine kinase. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Comment 2xHSV-tk was from RDB01692 pCM-TK
Vector backbone pCR Blunt II (plasmid)
Size of vector backbone 3.5 kb
Depositor|Developer Nakade, Koji |
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External Database
HSV HSV-tk
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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            Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

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            RDB18670 pCRtk2x2NN Under QC test. Please contact us. DNA solution

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            How to cite this biological resource

            Materials & Methods section:

            The pCRtk2x2NN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18670).

            Reference section:

            Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
            reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

            2024.04.04

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