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pUC-TEZ

tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.

Catalog number RDB18672
Resource name pUC-TEZ
Alternative name pUC19-TEBl
Clone info. tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector. The cassette will be liberated by SpeI/XbaI digestion. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Gene/insert name Discosoma sp. RFP (tdTomato) cDNA; Streptoalloteichus hindustanus bleomycin resistance gene.
Depositor|Developer Nakade, Koji |
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External Database
            Reference sequence
              
             
            Sequence (full) RDB18672hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB18672_B3Fep1-2.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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            提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。
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            RDB18672 pUC-TEZ DNA solution

            How to cite this biological resource

            Materials & Methods section:

            The pUC-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18672).

            Reference section:

            Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            References

            Original, user report and related articles

            original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
            reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

            2024.04.04

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