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ISM1 (N285Q) (#RDB18699)

Expression vector of human ISM1, mutant N285Q.

Clone info. Expression vector of human isthmin 1 (ISM1) tagged with Myc-His6 at C-terminal, mutant N285Q.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human ISM1 cDNA
Depositor|Developer Simizu, Siro |
 
Sequence (full) RDB18699hts01.seq checkAssembled from experimentally sequenced data.
Publication Yoshimoto, S., Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129840 (2021). [link to RRC of NBRP]
Test sheet RDB18699C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Yoshimoto S. et al., Biochim. Biophys. Acta-Gen. Subj., 1865 (3): 129840, 2021)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshimoto S. et al., Biochim. Biophys. Acta-Gen. Subj., 1865 (3): 129840, 2021)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18699 ISM1 (N285Q) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The ISM1 (N285Q) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18699).

Reference section:

Yoshimoto, S., Katayama, K., Suzuki, T., Dohmae, N., Simizu, S., Regulation of N-glycosylation and secretion of Isthmin-1 by its C-mannosylation. Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129840 (2021). PMID 33412225. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yoshimoto, S., Regulation of N-glycosylation and secretion of Isthmin-1 by its C-mannosylation. Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129840 (2021). PMID 33412225. [link to RRC of NBRP]