Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1 3xFLAG::LC3A

Expression vectors of human ATG8 homolog LC3A tagged with 3xFLAG at N-terminus.

Catalog number RDB19195
Resource name pcDNA3.1 3xFLAG::LC3A
Clone info. Expression vectors of human ATG8 homolog LC3A tagged with 3xFLAG at N-terminus.
Comment Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human MAP1LC3A cDNA
Depositor|Developer Yoshimori, Tamotsu |
Other clones in our bank

External Database
human MAP1LC3A

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a separate material transfer agreement with Osaka University.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          MTA, for use for not-for-profit academic purpose [Word]
          MTA, for use for for-profit purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020)。利用者が非営利目的の研究・教育以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19195 pcDNA3.1 3xFLAG::LC3A Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1 3xFLAG::LC3A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19195).

          Reference section:

          Nakamura, S., Shigeyama, S., Minami, S., Shima, T., Akayama, S., Matsuda, T., Esposito, A., Napolitano, G., Kuma, A., Namba-Hamano, T., Nakamura, J., Yamamoto, K., Sasai, M., Tokumura, A., Miyamoto, M., Oe, Y., Fujita, T., Terawaki, S., Takahashi, A., Hamasaki, M., Yamamoto, M., Okada, Y., Komatsu, M., Nagai, T., Takabatake, Y., Xu, H., Isaka, Y., Ballabio, A., Yoshimori, T., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol. 22 (10): 1252-1263 (2020). PMID 32989250. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content


          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Nakamura, S., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol. 22 (10): 1252-1263 (2020). PMID 32989250. [link to RRC of NBRP]

          2023.10.05

          GNP_filter3_RDBDEP_html_231001.pl