Resource data sheet
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C-Epac2-Y (#RDB19602)

A sensor for monitoring Epac2 (Rapgef4) activation in living cells.

Clone info. Expression vector of ECFP-Epac2A-EYFP, a sensor for monitoring Epac2 (Rapgef4) activation in living cells. See Zhang et al., Science, 325 (5940): 607-10, 2009.
Comment ECFP and EYFP are tagged at N- and C-terminus of mouse Epac2A respectively. Bgl-II/EcoRI fragment of Epac2A and EcoRI fragment of EYFP were ligated to Bgl-II/EcoRI sites of pECFP-C (Clontech). Expression was confirmed by the depositor with FRET imaging.
Vector backbone pECFP-C (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell).
Gene/insert name mouse Rapgef4 cDNA; Aequorea victoria GFP (ECFP, EYFP).
Depositor|Developer Takahashi, Harumi | Zhang, Chang-Liang |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Chang-Liang Zhang. et al., Science, 325(5940): 607-10, 2009)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chang-Liang Zhang. et al., Science, 325(5940): 607-10, 2009)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19602 C-Epac2-Y Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The C-Epac2-Y was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19602).

Reference section:

Zhang, C.L., Katoh, M., Shibasaki, T., Minami, K., Sunaga, Y., Takahashi, H., Yokoi, N., Iwasaki, M., Miki, T., Seino, S., The cAMP sensor Epac2 is a direct target of antidiabetic sulfonylurea drugs. Science 325 (5940): 607-610 (2009). PMID 19644119. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Zhang, C.L., The cAMP sensor Epac2 is a direct target of antidiabetic sulfonylurea drugs. Science 325 (5940): 607-610 (2009). PMID 19644119. [link to RRC of NBRP]