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Masato Kanemaki's Resource

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Catalog #Name of cloneRunning titlePublication
RDB18364pMK411TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18363pMK427TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-Nluc marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18362pMK312Expression vector of Cas9 and gRNA for a TOP2A tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18361pMK322Donor plasmid to generate TOP2A-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18360pMK321Donor plasmid to generate TOP2A-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18359BRD4-N CRISPRExpression vector of Cas9 and gRNA for a BRD4 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18358mAID-BRD4 donorDonor plasmid to generate BRD4-mAID degron cell lines, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18357SMC2 CRISPRExpression vector of Cas9 and gRNA for a SMC2 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18356SMC2-mAC donor (Hygro) Donor plasmid to generate SMC2-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18355CTCF-C CRISPRExpression vector of Cas9 and gRNA for a CTCF tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18354CTCF-mAC donor (Hygro) Donor plasmid to generate CTCF-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18353CTCF-mAC donor (Neo) Donor plasmid to generate CTCF-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18352DHC1 CRISPRExpression vector of Cas9 and gRNA for a DHC1 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18351DHC1-mIAA7 HygroDonor plasmid to generate DHC1-mlAA7 degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18350DHC1-mIAA7 NeoDonor plasmid to generate DHC1-mlAA7 degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18349pMK297Donor plasmid to generate DHC1-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18348pMK296Donor plasmid to generate DHC1-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18347pMK233A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18346RAD21 C-tag CIRPSRExpression vector of Cas9 and gRNA for a RAD21 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18345pMK265Donor plasmid to generate RAD21-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18344pMK262Donor plasmid to generate RAD21-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18343pMK425A plasmid for generate yeast lines expressing OsTIR1(F74A) under the GAL promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18342pMK419A plasmid for generate yeast lines expressing OsTIR1(F74G) under the GAL promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18341pMK198A plasmid for generate yeast lines expressing OsTIR1(wild type) under the GAL promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18340pAAV-hSyn-OsTIR1(F74A) Adeno-associated virus (AAV) expression vector of OsTIR1(F74A) and mAID-EGFP-NES marker with hSyn promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18339pAAV-hSyn-OsTIR1(F74G) Adeno-associated virus (AAV) expression vector of OsTIR1(F74G) and mAID-EGFP-NES marker with hSyn promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18338pAAV-hSyn-OsTIR1(WT) Adeno-associated virus (AAV) expression vector of OsTIR1(wild type) and mAID-EGFP-NES marker with hSyn promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18337pMK381A donor vector for introducing CMV-OsTIR1 (F74G mutant) at the human AAVS1 locus.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18336pAY5PiggyBac expression vector of mAID-EGFP-NLS marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18335pAY19TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18334pAY15TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB18333pAY7TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-NLS marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. (original). [link to RRC of NBRP]
RDB16826pMK409 (mAID-Nluc BSD)A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16825pMK408 (mAID-Nluc Hygro)A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16824pMK407 (BSD-P2A-mAID-Nluc)A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16823pMK401 (Hygro-P2A-mAID-Nluc)A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16822pMK394 (mAID-Nluc-Neo)A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16821pMK393 (mAID-mCherry2-BSD)A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16820pMK392 (mAID-mClover-BSD)A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16819pMK391 (mAID-BSD)A plasmid for construction of C-terminal mAID-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16818pMK390 (Stag-3FLAG-BSD)A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16817pMK389 (mCherry2-BSD)A plasmid for construction of C-terminal mCherry2-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16816pMK388 (mClover-BSD)A plasmid for construction of C-terminal mClover-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16815pMK377 (Hygro-P2A-mCherry2)A plasmid for construction of N-terminal Hygro-P2A-mCherry2 tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16814pMK376 (Hygro-P2A-mClover)A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16813pMK375 (Hygro-P2A-Stag-3FLAG)A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16811pMK364 (CMV-OsTIR1-loxP-PURO-loxP)A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16810pMK349 (BSD-P2A-mAID-mCherry2)A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16809pMK348 (BSD-P2A-mAID-mClover)A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16808pMK347 (BSD-P2A-mAID)A plasmid for construction of N-terminal BSD-P2A-mAID tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16807pMK346 (Hygro-P2A-mAID-mCherry2)A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16806pMK345 (Hygro-P2A-mAID-mClover)A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB16805pMK344 (Hygro-P2A-mAID)A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original). [link to RRC of NBRP]
RDB13937pMK294 (mAID-mCherry2-Bsr)A plasmid for construction of C-terminal mAID-mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13936pMK293 (mAID-mCherry2-Hygro)A plasmid for construction of C-terminal mAID-mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13935pMK292 (mAID-mCherry2-NeoR)A plasmid for construction of C-terminal mAID-mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13934pMK291 (mAID-mClover-Bsr)A plasmid for construction of C-terminal mAID-mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13933pMK290 (mAID-mClover-Hygro)A plasmid for construction of C-terminal mAID-mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13932pMK289 (mAID-mClover-NeoR)A plasmid for construction of C-terminal mAID-mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13931pMK288 (mAID-Bsr)A plasmid for construction of C-terminal mAID tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13930pMK287 (mAID-Hygro)A plasmid for construction of C-terminal mAID tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13929pMK286 (mAID-NeoR)A plasmid for construction of C-terminal mAID tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13928pMK285 (Stag-3FLAG-Bsr)A plasmid for construction of C-terminal Stag-3FLAG tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13927pMK284 (Stag-FLAG-Hygro)A plasmid for construction of C-terminal Stag-3FLAG tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13926pMK283 (Stag-3FLAG-Neo)A plasmid for construction of C-terminal Stag-3FLAG tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13925pMK282 (mCherry2-Bsr)A plasmid for construction of C-terminal mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13924pMK281 (mCherry2-Hygro)A plasmid for construction of C-terminal mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13923pMK280 (mCherry2-NeoR)A plasmid for construction of C-terminal mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13922pMK279 (mClover-Bsr)A plasmid for construction of C-terminal mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13921pMK278 (mClover-Hygro)A plasmid for construction of C-terminal mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13920pMK277 (mClover-NeoR)A plasmid for construction of C-terminal mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13918pMK232 (AAVS1 CMV-OsTIR1-PURO)A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB13917AAVS1 T2 CRISPR in pX330A CRISPR/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original). [link to RRC of NBRP]
RDB08470pMK107Expression clone for an auxin-based degron (AID) system, CENPH-aid.Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. (original). [link to RRC of NBRP]
RDB08469pMK106Expression clone for an auxin-based degron (AID) system, aid-CENPH.Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. (original). [link to RRC of NBRP]
RDB08468pNHK60Expression clone for an auxin-based degron (AID) system, GFP-aid-NLS.Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. (original). [link to RRC of NBRP]

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No Approval Forms Required for Fluorescent Protein Genes Developed by Dr. Atsushi Miyawaki
♦ RIKEN BRC will be closed from December 28, 2024 through January 5, 2025 due to New Year's holidays.
A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
Monomeric Fluorescent Protein Resource, mStayGold (Dec 18, 2023)
Visualization of Organelles update (Dec 18, 2023)
Development of two mouse strains conditionally expressing bright luciferases (Sep 08, 2023)
Autophagy and Mitophagy Updates (Aug 16, 2023)
High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
Plasmid of Cas9 expression/mRNA production, evaluation of the genome edit efficiency, and Knock-in donors and tags
Fucci cell cycle indicator, Calcium sensor and Fluorescent and Luminescent protein resources
Revision of Distribution Fees for Bioresources in RIKEN BRC
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2025.03.28

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