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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

YT101 (#RDB01181)

Human perforin cDNA

Alternative name Human perforin cDNA
Clone info. Human cDNA from YTN10 (Human NK-like clone) cDNA library
Vector backbone pBluescript SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human Perforin 1 cDNA
Depositor|Developer Okumura, Ko | Shinkai, Yoichi |
 
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Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01181 YT101 DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The YT101 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01181).

Reference section:

Shinkai, Y., Yoshida, M.C., Maeda, K., Kobata, T., Maruyama, K., Yodoi, J., Yagita, H., Okumura, K., Molecular cloning and chromosomal assignment of a human perforin (PFP) gene. Immunogenetics, 30, 452-457 (1989). PMID 2592021. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12400_A4Au.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12400_A4Aua.seq check
>RDB1181_12400_A4Au_2_M13(-40)_A01_01.ab1
    1 CCGCCGGTTA TTGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC CCCCCCTCGA
   61 GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTTTTT TTTTGATGCC ACCATTCCAG
  121 GAGCCTCGGT GAAGAGAGGA TATCCATCTG TGTAGCCGCT TCTCTATACG GGATTCCAGC
  181 TCCATGGCAG CCCGTCTGCT CCTCCTGGGC ATCCTTCTCC TGCTGCTGCC CCTGCCCGTC
  241 CCTGCCCCGT GCCACACAGC CGCACGCTCA GAGTGCAAGC GCAGCCACAA GTTCGTGCCT
  301 GGTGCATGGC TGGCCGGGGA GGGTGTGGAC GTGACCAGCC TCCGCCGCTC GGGCTCCTTC
  361 CCAGTGGACA CACAAAGGTT CCTGCGGCCC GACGGCACCT GCACCCTCTG TGAAAATGCC
  421 CTACAGGAGG GCACCCTCCA GCGCCTGCCT CTGGCGCTCA CCAACTGGCG GGCCCAGGGC
  481 TCTGGCTGCC AGCGCCATGT AACCAGGGCC AAAGTCAGCT CCACTGAAGC TGTGGCCCGG
  541 GATGCGGCTC GTAGCATCCG CAACGACTGG AAGGTCGGGC TGGACGTGAC TCCTAAGCCC
  601 ACCAGCAATG TGCATGTGTC TGTGGCCGGC TCACACTCAC AGGCAGCCAA CTTTGCAGCC
661 CAGAAGACCC ACCA
//
Primer:
Sequence file: RDB12400_A4Aub.seq check
>RDB1181_12400_A4Au_2_reverse2_B01_04.ab1
    1 CGGAAACATT GAATTACGCC AAGCTCGAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA
   61 GCTCCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCCT
  121 TTTTTTTTTT TTTTTTTTTG ATGATCACCA TAACATCATA TTTATTGGCC CTTTATCAAG
  181 CTATGTACAT GGTGAGACAG GTCAGGACAG GCCTCCATTT GTCTCAGGGG CAAAGCATTG
  241 TGGGCAAAGA AGACAGAGCA GCTGGAGCTG ATGGGGCTCC AGTTAAGGCA ATGAAGGCTT
  301 TGCCACACCA TAAAGGGCTC AAGGGAAGGG TCCTAAAAGA CCAGCCCTGA GCAGCCTGGT
  361 GGGAACAGCC TCTTGGCCTT CTGCCCAGCT CCTGGCTGAA GCTGTGATCT GTGTAGCTGT
  421 GACTGCAGGG CTTGAGAATG GCGGAGGGCT TAAGCAGTTT GGACAGGGTG AATCGGGATT
  481 AGCGTGTAAA CCCAGCCACC TCCCTGAAAA ACAGTCTGAA TCTCCCTTTT CCATCTGTCT
  541 GATGCGTATC CAATCTTTTG GCTTCTCTGG GCCACGTTGG AAGAAGAATT GTGTTGGGCC
601 ACATGTAAAA TCCACTAACA CTAACGATAG CCGATGAGCT AAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shinkai, Y., Molecular cloning and chromosomal assignment of a human perforin (PFP) gene. Immunogenetics, 30, 452-457 (1989). PMID 2592021. [link to RRC of NBRP]