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pHS102

E. coli K12 ruv operon

Catalog number RDB01544
Resource name pHS102
Alternative name E. coli K12 ruv operon
Clone info. ruvC encodes Holliday junction specific endonuclease. ruvA and ruvB encode Holliday junction specific DNA helicase.
Vector backbone pACYC177 (Plasmid)
Size of vector backbone 3.97 kb
Selectable markers Kan^r
Gene/insert name E. coli ruvA Genomic DNA E. coli ruvB Genomic DNA E. coli ruvC Genomic DNA
Depositor|Developer Shinagawa, Hideo |
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E. coli ruvA E. coli ruvB E. coli ruvC
      Reference sequence
        
       
      Remarks, protocol and/or map (gif) RDB01544.gif

      Distribution information

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      RDB01544 pHS102 Under QC test. Please contact us. DNA solution

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      How to cite this biological resource

      Materials & Methods section:

      The pHS102 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01544).

      Reference section:

      , Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev., 6, 2214-2220 (1992). PMID 1427081.
      Iwasaki, H., Takahagi, M., Shiba, T., Nakata, A., Shinagawa, H., Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J., 10, 4381-4389 (1991). PMID 1661673.
      Takahagi, M., Iwasaki, H., Nakata, A., Shinagawa, H., Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease. J. Bacteriol., 173, 5747-5753 (1991). PMID 1885548.
      Shinagawa, H., Makino, K., Amemura, M., Kimura, S., Iwasaki, H., Nakata, A., Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination. J. Bacteriol., 170, 4322-4329 (1988). PMID 2842314.

      Further references such as user reports and related articles (go to bottom)

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      QC test results

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      References

      Original, user report and related articles

      original , Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev., 6, 2214-2220 (1992). PMID 1427081.
      original Iwasaki, H., Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J., 10, 4381-4389 (1991). PMID 1661673.
      original Takahagi, M., Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease. J. Bacteriol., 173, 5747-5753 (1991). PMID 1885548.
      original Shinagawa, H., Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination. J. Bacteriol., 170, 4322-4329 (1988). PMID 2842314.

      2023.08.17

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