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pAxCACD

Shuttle vector to generate rAd expressing E. coli cytosine deaminase

Catalog number RDB01591
Resource name pAxCACD
Alternative name pAdex1CACD
Clone info. Shuttle vector to generate recombinant adenovirus vector expressing E. coli cytosine deaminase gene regulated by CA promoter.
Comment Produce adenovirus by COS-TPC method.
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name E. coli cytosine deaminase Genomic DNA
Depositor|Developer Hamada, Hirofumi |
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External Database
E. coli cytosine deaminase

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01591.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Cancer Gene Ther., 7, 1015-1022 (2000).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cancer Gene Ther., 7, 1015-1022 (2000).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB01591 pAxCACD Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pAxCACD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01591).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Suicide gene therapy (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          user_report Hamaji, Y., Strong enhancement of recombinant cytosine deaminase activity in Bifidobacterium longum for tumor-targeting enzyme/prodrug therapy. Biosci. Biotechnol. Biochem., 71, 874-883 (2007). PMID 17420581.
          user_report Nakamura T., Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors. Biosci. Biotechnol. Biochem., 66, 2362-2366 (2002). PMID 12506973.

          2023.05.02

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