Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

D-HA-pRc/CMV (#RDB02139)

Expressing a fusion protein tagged with duplicated HA, CMV promoter.

Alternative name D-HA, double HA-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated HA under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37 C, LB+Amp
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
 
Sequence RDB02139z.seq
Additional map
Remarks, protocol and/or map (pdf) RDB02139.pdf

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02139 D-HA-pRc/CMV DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The D-HA-pRc/CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02139).

Reference section:

Takemoto, Y., Sato, M., Furuta, M., Hashimoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14667_A7D6p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14667_A7D6a.seq check
>02139_14667_A7D6_CMV-Forward_A02_01_ABI08.ab1
    1 TGTACCAACT CCGCCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTTC CATGGCCTAC CCCTACGACG TGCCCGACTA
  181 CGCCGCGGCC TACCCCTACG ACGTGCCCGA CTACGCGGCC GCTCTAGAGG GCCCTATTCT
  241 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
  301 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
  361 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
  421 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
  481 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCGAG
  541 GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
  601 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
  661 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
  721 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
  781 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
  841 CTTTAATAGT GGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT
  901 CTTTTGATTT ATAAGGGATT TTTGCCGATT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT
  961 TTTAACAAAA ATTTTAACGC GAATTTTAAC AAAATATTTA ACGCCTTACC AATTAAATTA
 1021 TTTGCTTTAA TACCAATTCT TTCCTGTTTT GGGGCTTTTC TGATTAATCC AAACCCGGGG
 1081 TGGGTACCGA GGCTTCGAAA TCCTGTGATG GTGTGTCAGT TAGGGGTGTG GGAAAGGTTC
1141 CCCACAGGAC TC
//
Primer: SV40pro_F_V2
Sequence file: RDB14667_A7D6b.seq check
>02139_14667_A7D6_SV40pro_F_V2_B02_02_ABI08.ab1
    1 GGTCAGGGGT TTTTTTTGGA GGCCTAGGGC TTTTGCAAAA GCTCCCGGGC CCCTTGAGAT
   61 ATCCATTTTC GGATCTGATC AAAAAAAAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA
  121 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC
  181 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC
  241 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC
  301 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC
  361 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC
  421 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC
  481 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG
  541 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT
  601 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT
  661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG
  721 ATTCATCGAC TGTGGCCGGC TGGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
  781 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG
  841 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT
  901 GAGCGGGACT CTGGGGGTTT CGAAATGACC GACCCAAGCG ACGCCCCAAC CTGCCCATCA
  961 CGAGATTTTC GAATTTCCAC CGCCGCCTTC TATGGAAAGG GTTGGGCTTT CGGAAATCGT
 1021 TTCCCGGGAC GCCCGGGCTG GAATGGAATC CTTCCAAGCG CCGGGGAATC TTCAATGCCT
 1081 GGAGATTTCC TTTCGCCCCA ACCTCGAAAC TTGGTAATTG GCCAGGCTTA TAATGGGGTT
1141 TACCAAATAT AAAAGGCAAA TAAGGCAACT CCACGCCCGG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]
user_report Mitsui, S., Mental retardation-related protease, motopsin (prss12), binds to the BRICHOS domain of the integral membrane protein 2a. Cell Biol. Int., 38 (1): 117-123 (2014). PMID 23955961. [link to RRC of NBRP]
user_report Miura, E., VPS35 dysfunction impairs lysosomal degradation of alpha-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease. Neurobiol. Dis. 71: 1-13 (2014). PMID 25107340. [link to RRC of NBRP]
user_report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366. [link to RRC of NBRP]
user_report Hayashi, H., Regulation of intestinal Cl-/HCO3- exchanger SLC26A3 by intracellular pH. Am. J. Physiol. Cell Physiol., 296, C1279-1290 (2009). PMID 19321737. [link to RRC of NBRP]