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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGhp53WT (Forward) (#RDB02429)

Mammalian vector expressing wild type p53 gene

Clone info. Mammalian vector expressing wild type p53 gene
Vector backbone pCAG (Plasmid)
Selectable markers Amp^r
Gene/insert name Human tumor protein p53 cDNA
Depositor|Developer Yokoyama, Kazunari |
 
Sequence RDB02429y.seq
Sequence RDB02429z.seq
Additional map

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02429 pCAGhp53WT (Forward) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGhp53WT (Forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02429).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13743_A5Lop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: insert 5'
Sequence file: RDB13743_A5Loa.seq check
>02429_13743_A5Lo_pAxCA_F1_A04_01.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCCTTCTTCT TTTTNNNACN GCTCCTGGGN
   61 AACGNGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTTA TCGATTTAAT
  121 TCTAGAGCCA CCGTCCAGGG NGCAGGTAGC TGCTGGGCTC CGGGGNCACT TTGCGTTCGG
  181 GCTGGGAGCG TGCTTTCCAC GACGGTGACA CGCTTCCCTG GATTGGCAGC CAGACTGCCT
  241 TCCGGGTCAC TGCCATGGAG GAGCCGCAGT CAGATCCTAG CGTCGAGCCC CCTCTGAGTC
  301 AGGAAACATT TTCAGACCTA TGGAAACTAC TTCCTGAAAA CAACGTTCTG TCCCCCTTGC
  361 CGTCCCAAGC AATGGATGAT TTGATGCTGT CCCCGGACGA TATTGAACAA TGGTTCACTG
  421 AAGACCCAGG TCCAGATGAA GCTCCCAGAA TGCCAGAGGC TGCTCCCCCC GTGGCCCCTG
  481 CACCAGCAGC TCCTACACCG GCGGCCCCTG CACCAGCCCC CTCCTGGCCC CTGTCATCTT
  541 CTGTCCCTTC CCAGAAAACC TACCAGGGCA GCTACGGTTT CCGTCTGGGC TTCTTGCATT
  601 CTGGGACAGC CAAGTCTGTG ACTTGCACGT ACTCCCCTGC CCTCAACAAG ATGTTTTGCC
  661 AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGANTC CACACCCCCG CCCGGCACCC
  721 GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGANNNT GTGANGCGCT
  781 GCCCCCACCA TGANCGCTGC TCANATAGCG ATGGTCTGGC CCCTCCTCAN CATCTTATCC
  841 GANNGNNANN NAAATTNNCG NNNNNNNNAT TTGGNNGANA GNAACNCTTT TCNANATANN
901 GNNGNNNNNN NCCNANNANC CNNNNNNNNN N
//
Primer: pCA-bgter (Pr0066)
Region: insert 3'
Sequence file: RDB13743_A5Lob.seq check
>02429_13743_A5Lo_pCA-bgter_H02_23.ab1
    1 NNNNNNNNNN NNNNNNNNNT TTTGNNNNAN GGNNNNNNAN CTCNGTGGTA TTTGTGAGCC
   61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT
  121 TGATTATCGA TTCTAGACTA GTTTAATTAA TTAATTCCGG GACAAAGCAA ATGGAAGTCC
  181 TGGGTGCTTC TGACGCACAC CTATTGCAAG CAAGGGTTCA AAGACCCAAA ACCCAAAATG
  241 GCAGGGGAGG GAGAGATGGG GGTGGGAGGC TGTCAGTGGG GAACAAGAAG TGGAGAATGT
  301 CAGTCTGAGT CAGGCCCTTC TGTCTTGAAC ATGAGTTTTT TATGGCGGGA GGTAGACTGA
  361 CCCTTTTTGG ACTTCAGGTG GCTGGAGTGA GCCCTGCTCC CCCCTGGCTC CTTCCCAGCC
  421 TGGGCATCCT TGAGTTCCAA GGCCTCATTC AGCTCTCGGA ACATCTCGAA GCGCTCACGC
  481 CCACGGATCT GAAGGGTGAA ATATTCTCCA TCCAGTGGTT TCTTCTTTGG CTGGGGAGAG
  541 GANCTGGTGT TGTTGGGCAN TGCTCGCTTA NTGCTCCCTG GGGGCAGCTC GTGGTGAGGC
  601 TCCCCTTTCT TGCGGANATT CTCTTCCTCT GTGCGCCGGT CTCTCCCANG GANAGGCACN
661 NANANNCACC TCAAAGCTGT TCNNNNNNNN NN
//
Primer: TP53-M_F (Pr1029)
Region: insert mid(273aa,R)
Sequence file: RDB13743_A5Loc.seq check
>02429_13743_A5Lo_TP53-M_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN NTCNNCTACA ACTACNTGTG TAACAGTTCC TGCATGGGCG
   61 GCATGAACCG GAGGCCCATC CTCACCATCA TCACACTGGA AGACTCCAGT GGTAATCTAC
  121 TGGGACGGAA CAGCTTTGAG GTGCGTGTTT GTGCCTGTCC TGGGAGAGAC CGGCGCACAG
  181 AGGAAGAGAA TCTCCGCAAG AAAGGGGAGC CTCACCACGA GCTGCCCCCA GGGAGCACTA
  241 AGCGAGCACT GCCCAACAAC ACCAGCTCCT CTCCCCAGCC AAAGAAGAAA CCACTGGATG
  301 GAGAATATTT CACCCTTCAG ATCCGTGGGC GTGAGCGCTT CGAGATGTTC CGAGAGCTGA
  361 ATGAGGCCTT GGAACTCAAG GATGCCCAGG CTGGGAAGGA GCCAGGGGGG AGCAGGGCTC
  421 ACTCCAGCCA CCTGAAGTCC AAAAAGGGTC AGTCTACCTC CCGCCATAAA AAACTCATGT
  481 TCAAGACAGA AGGGCCTGAC TCAGACTGAC ATTCTCCACT TCTTGTTCCC CACTGACAGC
  541 CTCCCACCCC CATCTCTCCC TCCCCTGCCA TTTTGGGTTT TGGGTCTTTG AACCCTTGCT
  601 TGCNATANGT GTGCGTCAGA AGCACCCAGG ACTTCCATTT GCTTTGTCCN GGAATTAATT
  661 AATTAAACTA GTCNANNNTC NANNATCNAN TCACTNNNNN NNGCNNNNTN NNNATCNNNN
721 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles