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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGhp53R273H (Forward) (#RDB02433)

Mammalian vector expressing mutant of p53 gene (R273H)

Clone info. Mammalian vector expressing mutant of p53 gene
Vector backbone pCAG (Plasmid)
Selectable markers Amp^r
Gene/insert name Human tumor protein p53 cDNA
Depositor|Developer Yokoyama, Kazunari |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02433 pCAGhp53R273H (Forward) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGhp53R273H (Forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02433).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19202_B1G7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F (Pr0328)
Region: insert 5'
Sequence file: RDB19202_B1G7a.seq check
>02433_19202_B1G7_2_pCAG-F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA
   61 CCGGCGGCTC TANAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC
  121 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTG ATTTATCGAT
  181 TTAATTCTAG AGCCACCGTC CAGGGAGCAG GTAGCTGCTG GGCTCCGGGG ACACTTTGCG
  241 TTCGGGCTGG GAGCGTGCTT TCCACGACGG TGACACGCTT CCCTGGATTG GCAGCCAGAC
  301 TGCCTTCCGG GTCACTGCCA TGGAGGAGCC GCAGTCAGAT CCTAGCGTCG AGCCCCCTCT
  361 GAGTCAGGAA ACATTTTCAG ACCTATGGAA ACTACTTCCT GAAAACAACG TTCTGTCCCC
  421 CTTGCCNNNN NNNGCAATGG ATGATTTGAT GCTGTCCCCG GACGATATTG ANNNNNGGTT
  481 CACTGAAGAC CCANGTNNNN ANGAAGCTCC CANANNNNCA GAGGCTGCTC CCCCCGTGGC
  541 CCCTGCACCA NCNNCTCCTA CACCGGCGGC CCCTGCACCA GCCCCCNNCN TGGNCCCNNN
601 NNNNCTTCNG TCCCTTNNNN NNAAAANCTA NCNNNNNNNN NN
//
Primer: pCAGGS_R1 (Pr0614)
Region: insert 3'
Sequence file: RDB19202_B1G7b.seq check
>02433_19202_B1G7_2_pCAGGS_R1_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCNA TTGNNNTGAA AANAAATTTC CTTTATTANN NNNAAGTCAG
   61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG
  121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC
  181 CTGAGGAGTG AATTGATTAT CGATTCTAGA CTAGTTTAAT TAATTTAATT CCGGGACAAA
  241 GCAAATGGAA GTCCTGGGTG CTTCTGACGC ACACCTATTG CAAGCAAGGG TTCAAAGACC
  301 CAAAACCCAA AATGGCAGGG GAGGGAGAGA TGGGGGTGGG AGGCTGTCAG TGGGGAACAA
  361 GAAGTGGAGA ATGTCANNCN GAGTCANGCC CTTCTGTCNT GAACATGAGT TTTTTATGGC
421 GGGAGGTAGA NNGNNCCTTT TTGNANTNNN NNNNNNN
//
Primer: TP53-M_F (Pr1029)
Region: insert mid(273aa,H)
Sequence file: RDB19202_B1G7c.seq check
>02433_19202_B1G7_2_TP53-M_F_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNC NNNCNNCTAC NACTACNTGT GTAACAGTTC CTGCATGGGC
   61 GGCATGAACC GGAGGCCCAT CCTCACCATC ATCACACTGG AAGACTCCAG TGGTAATCTA
  121 CTGGGACGGA ACAGCTTTGA GGTGCACGTT TGTGCCTGTC CTGGGAGAGA CCGGCGCACA
  181 GAGGAAGAGA ATCTCCGCAA GAAAGGGGAG CCTCACCACG AGCTGCCCCC AGGGAGCACT
  241 AAGCGAGCAC TGCCCAACAA CACCAGCTCC TCTCCCCAGC CAAAGAAGAA ACCACTGGAT
  301 GGAGAATATT TCACCCTTCA GATCCGTGGG CGTGAGCGCT TCGAGATGTT CCGAGAGCTG
  361 AATGAGGCCT TGGAACTCAA GGATGCCCAG GCTGGGAAGG AGCCAGGGGG GAGCAGGGCT
  421 CACTCCAGCC ACCTGAAGTC CAAAAAGGGT CAGTCTACCT CCCGCCATAA AAAACTCATG
  481 TTCAAGACAG AAGGGCCTGA CTCAGACTGA CATTCTCCAC TTCTTGTTCC CCACTGACAG
  541 CCTCCCACCC CCATCTCTCC CTCCCCTGCC ATTTTGGGTT TTGGGTCTTT GAACCCTTGC
  601 TTGCAATAGG TGTGCGTCAG AAGCACCCAG GACTTCCATT TGCTTTGTCC CGGAATTAAA
  661 TTAATTAAAC TAGTCNAGAA NCNANNNTCN NNTCNCTCCT CNNNGCNNNN TNNNNATCNN
721 NNNNNNGNNN CNNGNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles