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AxCAhNF-IL6 beta (forward) (#RDB02747)

Recombinant adenovirus harboring human NF-IL6 beta cDNA

Clone info. Recombinant adenovirus harboring human NF-IL6 beta cDNA. Human NF-IL6 beta is cloned into the SwaI site of pAxCAwt. (Direction:forward)
Comment Expression of proteins was not determined at the production of this virus.
Vector backbone pAxCAwt (Adenovirus)
Growth remarks 5 percent FCS is fine.
Gene/insert name Human NF-IL6 beta cDNA
Depositor|Developer DNA Bank, |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Please ensure RCAs are not in stocks prior to use of recombinant adenoviruses. (https://brc.riken.jp/dna/GRP0046e)
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 【追加提供依頼書】受入れ確認書(書式J)
実験に使用する前に、タイターを上げた試料中にRCAが混入していないことを確認してください。(https://brc.riken.jp/dna/GRP0046j)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB02747 AxCAhNF-IL6 beta (forward) Under QC test. Please contact us. Virus, lysate of infected cell culture Please contact us.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The AxCAhNF-IL6 beta (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02747).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

reference Bu, D.F., Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene. Proc. Natl. Acad. Sci. USA, 89, 1473 (1992). PMID 1549570.