Resource data sheet
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AxCAhp53WT (forward) (#RDB02755)

Recombinant adenovirus harboring human p53 cDNA

Clone info. Recombinant adenovirus harboring human p53 cDNA. Human wild type p53 is cloned into the SwaI site of pAxCAwt. (Direction:forward)
Comment Expression was confirmed by a recipient investigator by Western blotting.
Vector backbone pAxCAwt (Adenovirus)
Growth remarks 5 percent FCS is fine.
Gene/insert name Human tumor protein p53 cDNA
Depositor|Developer Yokoyama, Kazunari |
 
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

MTAに書く使用条件 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 【追加提供依頼書】受入れ確認書(書式J)
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB02755 AxCAhp53WT (forward) Under QC test. Please contact us. Virus, lysate of infected cell culture Please contact us.

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How to cite this biological resource

Materials & Methods section:

The AxCAhp53WT (forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02755).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

user_report Ichwan S.J., Defect in serine 46 phosphorylation of p53 contributes to acquisition of p53 resistance in oral squamous cell carcinoma cells. Oncogene, 25, 1216-1224 (2006). PMID 16247456. [link to RRC of NBRP]