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AxCALNLrAAT-RVRR

Recombinant adenovirus harbouring rat alpha1-antitrypsin cDNA

Catalog number RDB03210
Resource name AxCALNLrAAT-RVRR
Alternative name 1159 3S.4
Clone info. Recombinant adenovirus harbouring rat alpha1-antitrypsin cDNA. RVRR mutant form.
Comment Expression of proteins was not determined at the production of this virus.
Vector backbone pAxCALNLw (Adenovirus)
Growth remarks 5 percent FCS is fine.
Gene/insert name Rat alpha1-antitrypsin cDNA
Depositor|Developer DNA Bank, |
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External Database
Rat alpha1-antitrypsin

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuji et al. J. Biochem, 126, 591-603 (1999); Tsuji et al. Protein Engng., 15(2), 123-130 (2002).)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsuji et al. J. Biochem, 126, 591-603 (1999); Tsuji et al. Protein Engng., 15(2), 123-130 (2002).)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          備考 【追加提供依頼書】受入れ確認書(書式J)
          組換え体提供にかかる書式が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB03210 AxCALNLrAAT-RVRR Under QC test. Please contact us. Virus, lysate of infected cell culture Please contact us.

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB03210.gif

          Original reference

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          reference Tsuji, A., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148.
          reference Tsuji, A., Inactivation of proprotein convertase, PACE4, by alpha1-antitrypsin Portland (alpha1-PDX), a blocker of proteolytic activation of bone morphogenetic protein during embryogenesis: evidence that PACE4 is able to form an SDS-stable acyl intermediate with alpha1-PDX. J. Biochem, 126, 591-603 (1999). PMID 10467177.

          2022.05.18

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