Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAxCALNLhCCNA1 (forward)

Shuttle vector to generate rAd harboring human CCNA1 (forward)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB03534_B0Fmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCLNLW_F Region: SV40 pA, loxP, insert 5' Sequence file: RDB03534_B0Fma.seq
>03534_03534_B0Fm_1_pAxCLNLW_F_A07_01_ABI24.ab1     1 NNNNNNNNNN NNGCNNNNNN NNCNTCNNGC GCGGGGNNCT CNTGCTGGNN TTCTTCGCCC    61 ACCCCATCGA TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA   121 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA   181 TCAATGTATC TTATCATGTC TGGATCAATC CGAACGCGTA TAACTTCGTA TAGCATACAT   241 TATACGAAGT TATCTCGAGT CGCTCGGTAC GATTTTCGAG CACTGTTGGC CTACTGGAGT   301 TGTTCCGGAC ACATAGAAAG ATAACGACGG GAAGAGCGGG GCCCGCTTTG GGGTCCAGGC   361 AGGTTTTGGG GCCTCCTGTC TGGTGGGAGG AGGCCGCAGC GCAGCACCCT GCTCGTCACT   421 TGGGATGGAG ACCGGCTTTC CCGCAATCAT GTACCCTGGA TCTTTTATTG GGGGCTGGGG   481 AGAAGAGTAT CTCAGCTGGG AAGGACCGGG GCTCCCAGAT TTCGTCTTCC AGCAGCAGCC   541 CGTGGAGTCT GAAGCAATGC ACTGCAGCAA CCCCAAGAGT GGAGTTGTGC TGGCTACAGT   601 GGCCCGAGGT CCCGATGCTT GTCAGATACT CACCAGAGCC CCGCTGGGCC AGGATCCCCC   661 GCAGAGGACA GTGCTAGGGC TGCTAACTGC AAATGGGCAG TACAGGANGA NCTGTGGCCA   721 GGGGATCACA AGAATCANGT GTTATTCTGG ATCAGAAAAT GCCTTCCCTC CNGCTGGAAA   781 GAAAGCACTC CCTGACTGTG GGGGNNCAAG ANCCCCCCCA AGCAAGGGTT TGACATCTAC   841 ATGGNTGAAC TANANCNNGG GGGANAGANA CNGCTGCTCN GNNCAGANAN GGGGANGGNN   901 TTTNANGNNG NGNATGAAGN ANANNANTTT GNCTNNNNNN NNNANNANAC ATACANNAAA 961 NNNNNNNNNN //
Primer: hCCNA1-M_F Region: insert 3' Sequence file: RDB03534_B0Fmb.seq
>03534_03534_B0Fm_1_hCCNA1-M_F_D06_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC NGCNTTNNCN GNGNANTCNT TAAGTGAAAT TGTGCCTTGC    61 CTGANTGAGC TTCATAAAGC GTACCTTGAT ATACCCCATC GACCTCAGCA AGCAATTAGG   121 GAGAAGTACA AGGCTTCAAA GTACCTGTGT GTGTCCCTCA TGGAGCCACC TGCAGTTCTT   181 CTTCTACAAT AAGTTTCTGA ATGGAAGCAC TTCCGGAACT TCACCTCCAT ATCAGAAGTG   241 CCAATAATCG TCATAGGCTT CTGCACGTTG GATCAACTAA TGTTGTTTAC AATATAGATG   301 ACATTTTAAA AATGTAAATG AATTTAGTTT CCCTTAGACT TTAGTAGTTT GTAATATAGT   361 CCAACATTTT TTAAACAATA AACTGCTTGT CTTATGAAAA AAAAAAAAAA AAAAAAAAAA   421 AAAAAGGCCA CATGTGCTCG AAAATCAATT CACTCCTCAG GTGCAGGCTG CCTATCANAA   481 GGTGGTGGCT GGTGTGGCCA ATGCCCTGGC TCACAAATAC CACTGANATC TTTTTCCCTC   541 TGCCAAAAAT TATGGGGACA TCATGAAGCC CCTTGAGCAT CTGACTTCTG GCTAATAAAG   601 GAAATTTATT TTCATTGCAA TAGTGTGTTG GAATTTTTTG TGTCTCTCAC TCGGAAGGAC   661 ATATGGGAGG GCAAATCATT TAAAACATCA NAATGAGTAT TTGGTTNANA GTTTGGCAAC   721 ATATGCCCAT ATGCTGGCTG CCATGAACAA AGGTTGGCTA TAAAGAGGTC ATCAGTATAT   781 GAAACAGCCC CCCNGCTGNC CATTCNTNAT TCCATANAAA ANCCTTGACT NGANGNTANA   841 NTTTTTTTTN ANATTTTGTT TTGNGNNANT TTTTTNNNTN NACATNCCCT AAAAATTTTN   901 CNTTANNNNT TTTNANNANN CNAGANTTTT TNCNNNCNNN NNCNNNANNN NNNCCNANNN 961 NNNANNNNNN NCNNNNTNNN NNNNNNN //
Primer: tetpro-B_R Region: ITR, Ad5 psi Sequence file: RDB03534_B0Fmc.seq
>03534_03534_B0Fm_1_tetpro-B_R_D07_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNAATGNN GGGGTGGNNT TTGTGNNGTG GCGCGGGGCG    61 TGGGAACGGG GCGGGTGNNG TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA   121 ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG   181 GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG CGTAACCGAG   241 TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA AGTGAAATCT GAATAATTTT   301 GTGTTACTCA TAGCGCGTAA TATTTGTCTA GGGCCGCGGG GACTTTGACC GTTTACGTGG   361 AGACTCGCCC AGGTGTTTTT CTCAGGTGTT TTCCGCGTTC CGGGTCAAAG TTGGCGTTTT   421 ATTATTATAG TCAGCATCGA TTTAGCTTGG GCTGCAGGTC GAGGGATCTC CATAAGAGAA   481 GAGGGACAGC TATGACTGGG ANTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT   541 GTAAGGAAAA TTTTAGGGAT GTTAAAGAAA AAAATAACAC AAAACAAAAT ATAAAAAAAA   601 TCTAACCTCN ANGTCAAGGC TTTTCTATGG AATAAGGAAT GGACAGCANG GGGCTGTTTC   661 ATATACTGAT GACCTCTTTA TAGCCAACCT TTGTTCATGG CAGCCAGCAT ATGGGCATAT   721 GTTGCCNAAC TCTAAACCNA ATACTCATTC TGATGTTTTA AATGANTTGC CCTCCCATAT   781 GTCCNTCCGA GTGANANACA CAAAAAAAAT TCCNACACAC TATTGCAATG AAAATAANTT   841 TCCTTTTATT ANNCAGAAAN NCANATGCTC AANGGGCTTC NTGATGTCCC CATANTTTTT   901 GGNANANGGN AAAAGATNNC ANNGGNNATT NNNGANCCAG GGNATNNGNN NNCNCNNNCN 961 NNCNNCNNTN NNGNNANGNN NNNCNNGNNN NNNNNNN //
Primer: pCAG-F_V2 Region: CAG pro, loxP, NeoR Sequence file: RDB03534_B0Fmd.seq
>03534_03534_B0Fm_1_pCAG-F_V2_C06_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GNGCNNNNNN GANTCTGGGN NGCGCCGCCG CACCCCCTCT    61 AGCGGGCGCG GGGCGNANCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT   121 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CCTCTCCAGC CTCGGGGCTG TCCGCGGGGG   181 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC   241 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC   301 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTAA TTCGAGCGAA   361 CGCGTATAAC TTCGTATAGC ATACATTATA CGAAGTTATC TCGAGTCGGA TTTGATCTGA   421 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC   481 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG   541 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC   601 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC   661 CACGACGGGC GTTCCTTGCG CANCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG   721 GCTGCTATTG GGCGAAGTGC CGGGGCANGA TCTCCTGTCA TCTCACCTTG CNCCTGCNGA   781 GAAAGTATCC ATCATGGCTG ANGCNATGCG GCGGCTGCAT ACGCTTGATN CGGCTANCNG   841 CCCATTCGAN CACNNAGCNA ANCATCGNAN NCNNNCGNNC NCGTACTNNG ATGNAAANNN   901 NGNNTNNNNN ATNNNNNANN NTCNGGNNNN AANNNNNTNN NNGGCNNNNN GNNNNNCNNN 961 ANNTNNTNNN NNNNGNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl