Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAxCALNLhCCNA1 (forward)

Shuttle vector to generate rAd harboring human CCNA1 (forward)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB03557_B0Fmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCLNLW_F Region: SV40 pA, loxP, insert 5' Sequence file: RDB03557_B0Fma.seq
>03557_03557_B0Fm_3_pAxCLNLW_F_A05_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCTCNNG CGCGGGGNNC TCNTGCTGGN GTTCTTCGCC    61 CACCCCATCG ATAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC   121 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC   181 ATCAATGTAT CTTATCATGT CTGGATCAAT CCGAACGCGT ATAACTTCGT ATAGCATACA   241 TTATACGAAG TTATCTCGAG TCGCTCGGTA CGATTTTCGA GCACTGTTGG CCTACTGGCT   301 CCCAGGCTGG AATCCCTCGA CACTTGGTCC TTCCCGCCCC GCCCTTCCGT GCCCTGCCCT   361 TCCCTGCCCT TCCCCGCCCT GCCCCGCCCG GCCCGGCCCG GCCCTGCCCA ACCCTGCCCC   421 GCCCTGCCCC GCCCAGCCGG CCACCTCTTA ACCGCGATCC TCCAGTGCAC TTGCCAGTTG   481 TTCCGGACAC ATAGAAAGAT AACGACGGGA AGAGCGGGGC CCGCTTTGGG GTCCAGGCAG   541 GTCTTGGGGC CTCCTGTCTG GTGGGAGGAG GCCGCAGCGC AGCACCCTGC TCGTCACTTG   601 GGATGGAGAC CGGCTTTCCC GCAATCATGT ACCCTGGATC TTTTATTGGG GGCTGGGGAG   661 AAGAGTATCT CAGCTGGGAA GGANCGGGGC TCCCAGATTT CGTCTTCCAG CAGCCCGTGG   721 AGTCTGAAGC AATGCACTGC AGCAACCCCA AGAGTGGAGT TGTGCTGGCT ACAGTGGCCC   781 GAGGTCCCGA TGCTTGTCAG ATACTCACCA GANCCCCGCT GGGCCNGGAT NCCCCGCAGA   841 NGNNAGTGCT ANGGCTGCTN NNNGNNAATG GGCAGTACAG GANGNNCTGN GGCNNGGGGN   901 NCACNANAAT CANGNGNTNN TCTGGATCAN NAAATGNNNN NCNNCCNNCN NGAAANAAAG 961 CACTNNNNNN NNNN //
Primer: pCAGGS_R1 Region: beta-globin pA, insert 3' Sequence file: RDB03557_B0Fmb.seq
>03557_03557_B0Fm_3_pCAGGS_R1_B05_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN TGNNNNNAAT TTCCTTTATT AGCCAGAAGT    61 CAGATGCTCA AGGGGCTTCA TGATGTCCCC ATAATTTTTG GCAGAGGGAA AAAGATCTCA   121 GTGGTATTTG TGAGCCAGGG CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG   181 CACCTGAGGA GTGAATTGAT TTTCGAGCAC ATGTGGCCTT TTTTTTTTTT TTTTTTTTTC   241 ACAAGACAAG CAGTTTATTG TTTAAAAAAT GTTGGACTAT ATTACAAACT ACTAAAGTCT   301 AAGGGAAACT AAATTCATTT ACATTTTTAA AATGTCATCT ATATTGTAAA CAACATTAGT   361 TGATCCAACG TGCAGAAGCC TATGACGATT ATTGGCACTT CTGATATGGA GGTGAAGTTC   421 TGGAAGTGCT TCCATTCANA AACTTATTGT ANAANAANAA CTGCAGGTGG CTCCATGAGG   481 GACACACACA GGTACTTTGA AGCCTTGTAC TTCTCCCTAA TTGCTTGCTG AGGTCGATGG   541 GGTATATCAA GGTACGCTTT ATGAAGCTCA CTCAGGCAAG GCACAATTTC ACTTAATGAA   601 TACCCTGTAA ATGCAGCAAG GGTTTCTGGC CAAAAGTGCT TGTTCACAGT ATAGTTTGCC   661 AGGCAAAAAG CTGCTGCAGC TATCAGTGAA GGAAGATATT TCAAGANTGG ATCTGCTTCA   721 AGTAGACTCA GCTCTGCTAC GTACTTAGCC AGGTTCTCAG NCCTGACNCA CACTCCTTGT   781 CGCCTCAAGT ACTGANNGAN AAANTGGTNG GNGGNTGGTN CTGTCANATC AAAAGCTANA   841 ANTTTNANAA NCAAGTGTTC CNNTTTTANN AGTTGTCNNT TTGNGTATGT NTCNTNNNNN   901 ATANNNNNNN AANTCNNNCT ACTTNNNNNA NNNNATNTCT CTNNATNNTT NNGANNNNNN 961 AANNNNNNNN NN //
Primer: tetpro-B_R Region: ITR, Ad5 psi Sequence file: RDB03557_B0Fmc.seq
>03557_03557_B0Fm_3_tetpro-B_R_D04_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNTGNN NNNGNNGGGG TGGNGTTTGT GNNGTGGCGC GGGGCGTGGG    61 ANCGGGGCGG GTGNNGTAGT AGTGTGGCGG AAGTGTGATG TTGCAAGTGT GGCGGAACAC   121 ATGTAAGCGA CGGATGTGGC AAAAGTGACG TTTTTGGTGT GCGCCGGTGT ACACAGGAAG   181 TGACAATTTT CGCGCGGTTT TAGGCGGATG TTGTAGTAAA TTTGGGCGTA ACCGAGTAAG   241 ATTTGGCCAT TTTCGCGGGA AAACTGAATA AGAGGAAGTG AAATCTGAAT AATTTTGTGT   301 TACTCATAGC GCGTAATATT TGTCTAGGGC CGCGGGGACT TTGACCGTTT ACGTGGAGAC   361 TCGCCCAGGT GTTTTTCTCA GGTGTTTTCC GCGTTCCGGG TCAAAGTTGG CGTTTTATTA   421 TTATAGTCAG CATCGATTTA GCTTGGGCTG CAGGTCGAGG GATCTCCATA AGAGAAGAGG   481 GACAGCTATG ACTGGGAGTA GTCAGGAGAG GAGGAAAAAT CTGGCTAGTA AAACATGTAA   541 GGAAAATTTT AGGGATGTTA AAGAAAAAAA TAACACAAAA CAAAATATAA AAAAAATCTA   601 ACCTCAAGTC AAGGCTTTTC TATGGAATAA GGAATGGACA GCAGGGGGCT GTTTCATATA   661 CTGATGACCT CTTTATAGCC AACCTTTGTT CATGGCAGCC AGCATATGGG CATATGTTGC   721 CAAACTCTAA ACCAAATACT CATTCTGATG TTTTAAATGA TTTGCCCTCC CATATGTCCT   781 TCCNAGTGAG AGACACAAAA AATTCCAACA CACTATTGCA ATGAAAATAA ATTTCCTTTA   841 TNAGCNNGAA NTCAGANGCT CAANGGGCTT CATGANGTCC CCNTAATTTT TGGCAGANGG   901 AAAAANATNN CANNGGNNTT TGTGANNCAG GGNNTTNGNC ACACCANNCN NCCNNCNTNN 961 TGANNNNNNN NCNNNNNNNN NN //
Primer: pAxCA_F1 Region: loxP, NeoR Sequence file: RDB03557_B0Fmd.seq
>03557_03557_B0Fm_3_pAxCA_F1_C04_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCTTCTTC TTTTTCCNNN NNNCTCCTGG    61 GCAACGTGCT GGTTATTGTG CTGNCTCATC ATTTTGGCAA AGAATTGNNN NNTTCGAGCG   121 AACGCGTATA ACTTCGTATA GCATACATTA TACGAAGTTA TCTCGAGTCG GATTTGATCT   181 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT   241 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC   301 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG   361 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG   421 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC   481 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC   541 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC   601 TGCCCATTCG ACCACCAAGC GAANCATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC   661 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GANCATCANG GGCTCGCGCC AGCCGAACTG   721 TTCGCCAGGC TCNANNGCGC GCATGCCCNA CGGCGANGAT CTCGTCGNGN NCCATGGCGA   781 TGCCTGCTTG CCNAATATCA TGGTGGAAAA NGGCCGCTTT TCTGGNNTCA TCGACNGNGG   841 CCGGCTGGGN GTGGCGGANN GCTATCAGGA CATANCGNTN NGCTACCCNN GATATTGNTN   901 ANNANNNTNG GCGGCNAATG GGNNTGANNN NNTNNCNNCN NGCTTTNNNG NATCGNNGNT   961 CCNNATTCGC ANCNNNNTCN NNNTTNTANN GNNNTTNNTN NNNNNNNNTN NNTNNNGNNN 1021 NNGNNNNNNN NGGNNNNNNN NAANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl