Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAhIL12p40

Expression vector of human IL-12 p40 cDNA

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15737_A8Ahp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 Sequence file: RDB15737_A8Aha.seq
>03959_15737_A8Ah_pCAG-F_V2_E01_05_ABI08.ab1     1 GCGGATGGTT GTTTCAATCT GGTGCGGAGC CGAATCTGGG AGGCGCCGCC GCACCCCCTC    61 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT   121 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG   181 GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG   241 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG   301 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCTAGA CTGCCATGTG   361 TCACCAGCAG TTGGTCATCT CTTGGTTTTC CCTGGTTTTT CTGGCATCTC CCCTCGTGGC   421 CATATGGGAA CTGAAGAAAG ATGTTTATGT CGTAGAATTG GATTGGTATC CGGATGCCCC   481 TGGAGAAATG GTGGTCCTCA CCTGTGACAC CCCTGAAGAA GATGGTATCA CCTGGACCTT   541 GGACCAGAGC AGTGAGGTCT TAGGCTCTGG CAAAACCCTG ACCATCCAAG TCAAAGAGTT   601 TGGAGATGCT GGCCAGTACA CCTGTCACAA AGGAGGCGAG GTTCTAAGCC ATTCGCTCCT   661 GCTGCTTCAC AAAAAGGAAG ATGGAATTTG GTCGACTGAT ATTTTAAAGG ACCAGAAAGA   721 ACCCAAAAAT AAGACCTTTC TAAGATGCGA GGCCAAGAAT TATTCTGGAC GTTTCACCTG   781 CTGGTGGCTG ACGACAATCA GTACTGATTT GACATTCAGT GTCAAAAGCA GCAGAGGCTC   841 TTCTGACCCC CAAGGGGTGA CGTGCGGAGC TGCTACACTC TCTGCAGAGA GAGTCAGAGG   901 GGACAACAAG GAGTATGAGT ACTCAGTGGA GTGCCAGGAG GACAGTGCCT GCCCAGCTGC   961 TGAGGAGAGT CTGCCCATTG AGGTCATGGT GGATGCCGTT CACAAGCTCA AGTATGAAAA  1021 CTACACCAGC AGCTTCTTCA TCAGGACATC ATCAAACCTG ACCCACCCAG ACTTGCAGCT  1081 GAGCCATTAA AGAATTCTCG CAGGTGAGTC AGCTGGGGAG TACTGGACAC TTGAAGTACT 1141 TCACATTCCT TACCTTCTCC TTGACACTTC TGCGTTCAGG GTTCCAAGG //
Primer: pCA-bgter Sequence file: RDB15737_A8Ahb.seq
>03959_15737_A8Ah_pCA-bgter_F01_06_ABI08.ab1     1 AACCCGGAAG GTCCCATAAT TTTTGGCAGA GGGAAAAGAT CTCAGTGGTA TTTGTGAGCC    61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT   121 TCTAGAGGAT CGATCCTAAC TGCAGGGCAC AGATGCCCAT TCGCTCCAAG ATGAGCTATA   181 GTAGCGGTCC TGGGCCCGCA CGCTAATGCT GGCATTTTTG CGGCAGATGA CCGTGGCTGA   241 GGTCTTGTCC GTGAAGACTC TATCTTTCTT TTCTCTCTTG CTCTTGCCCT GGACCTGAAC   301 GCAGAATGTC AGGGAGAAGT AGGAATGTGG AGTACTCCAG GTGTCAGGGT ACTCCCAGCT   361 GACCTCCACC TGCCGAGAAT TCTTTAATGG CTTCAGCTGC AAGTTCTTGG GTGGGTCAGG   421 TTTGATGATG TCCCTGATGA AGAAGCTGCT GGTGTAGTTT TCATACTTGA GCTTGTGAAC   481 GGCATCCACC ATGACCTCAA TGGGCAGACT CTCCTCAGCA GCTGGGCAGG CACTGTCCTC   541 CTGGCACTCC ACTGAGTACT CATACTCCTT GTTGTCCCCT CTGACTCTCT CTGCAGAGAG   601 TGTAGCAGCT CCGCACGTCA CCCCTTGGGG GTCAGAAGAG CCTCTGCTGC TTTTGACACT   661 GAATGTCAAA TCAGTACTGA TTGTCGTCAG CCACCAGCAG GTGAAACGTC CAGAATAATT   721 CTTGGCCTCG CATCTTAGAA AGGTCTTATT TTTGGGTTCT TTCTGGTCCT TTAAAATATC   781 AGTCGACCAA ATTCCATCTT CCTTTTTGTG AAGCAGCAGG AGCGAATGGC TTAGAACCTC   841 GCCTCCTTTG TGACAGGTGT ACTGGCCAGC ATCTCCAAAC TCTTTGACTT GGATGGTCAG   901 GGTTTTGCCA GAGCCTAAGA CCTCACTGCT CTGGTCCAAG GTCCAGGTGA TACCATCTTC   961 TTCAGGGGTG TCACAGGTGA GGACCACCAT TTCTCCAGGG GCATCCGGAT ACCAATCCCA  1021 ATTCTACGAC ATAAACATCT TTCTTCAGTT CCCATATGGG CCACGAGGGG AGATGCCAGA  1081 AAAACAGGGA AAACCAGGAG ATGACCAACT GCTGTGACAC ATGGCAGGTC TAGGAGTTCT  1141 TGCCCAAAAT GATGAAGACA GCCACATTAC TAGCACGTTG GCCAGAGCGT TAGGAAGGTA 1201 GAGACCATGT GTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl