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CSII-CMV-MCS-IRES2-Venus (#RDB04383)

Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.


Drawn by SnapGene® software
Sequence (full) RDB04383hts01.seq
Remarks, protocol and/or map (pdf) RDB04383.pdf
Publication Unpublished bioresource
Test sheet RDB17904_B0Aup1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-MCS (RDB04377) (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 30C, LB+Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04383 CSII-CMV-MCS-IRES2-Venus DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04383).

Reference section:

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

reference Ikemoto, M., Autologous transplantation of SM/C-2.6(+) satellite cells transduced with micro-dystrophin CS1 cDNA by lentiviral vector into mdx mice. Mo.l Ther. 15 (12): 2178-2185 (2007). PMID 17726457.
user_report Suzuki, Y., Identification of APBB1 as a substrate for anaplastic lymphoma kinase. J. Biochem. 176 (5): 395-403 (2024). PMID 39115278. [link to RRC of NBRP]
user_report Nakamura, K., The periosteum provides a stromal defence against cancer invasion into the bone. Nature 634 (8033): 474-481 (2024). PMID 39169177. [link to RRC of NBRP]
user_report Mizutani, T., Neutrophil S100A9 supports M2 macrophage niche formation in granulomas. iScience 26 (3): 106081 (2023). PMID 36843852. [link to RRC of NBRP]
user_report Watanabe ,Y., Insulin-induced genes INSIG1 and INSIG2 mediate oxysterol-dependent activation of the PERK-eIF2α-ATF4 axis. J. Biol. Chem. 297 (2): 100989 (2021). PMID 34298014. [link to RRC of NBRP]
user_report Kikuchi, J., Soluble SLAMF7 promotes the growth of myeloma cells via homophilic interaction with surface SLAMF7. Leukemia 34 (1): 180-195 (2020). PMID 31358854. [link to RRC of NBRP]
user_report Ishibashi, D., Type I interferon protects neurons from prions in in vivo models. Brain 142 (4): 1035-1050 (2019). PMID 30753318. [link to RRC of NBRP]
user_report Hui, K.K., GABARAPs dysfunction by autophagy deficiency in adolescent brain impairs GABAA receptor trafficking and social behavior. Sci. Adv. 5 (4): eaau8237 (2019). PMID 30989111. [link to RRC of NBRP]
user_report Watanabe, N., A pull-down and slot blot-based screening system for inhibitor compounds of the podoplanin-CLEC-2 interaction. PLoS One 14 (9): e0222331 (2019). PMID 31553741. [link to RRC of NBRP]
user_report Kikuchi, J., Myeloma Cells are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex which Senses Lipopolysaccharide. Cancer Res. 78 (7): 1766-1778 (2018). PMID 29363546. [link to RRC of NBRP]
user_report Osada, N., Lysine-specific demethylase 1 inhibitors prevent teratoma development from human induced pluripotent stem cells. Oncotarget 9 (5): 6450-6462 (2018). PMID 29464084. [link to RRC of NBRP]
user_report Uenaka, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437. [link to RRC of NBRP]
user_report Tanaka, M., Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease. J. Clin. Invest. 127 (4): 1438-1450 (2017). PMID 28263187. [link to RRC of NBRP]
user_report Su, Z., Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes. Oncotarget 8 (63): 106296-106310 (2017). PMID 29290949. [link to RRC of NBRP]
user_report Sasamoto, Y., PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes. Sci. Rep. 6: 20807, 2016. PMID 26899008. [link to RRC of NBRP]
user_report Lu, F., Downregulation of CREB Promotes Cell Proliferation by Mediating G1/S Phase Transition in Hodgkin Lymphoma. Oncol. Res. 24 (3): 171-179 (2016). PMID 27458098. [link to RRC of NBRP]
user_report Hikita, A., Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy. Bone 76: 5-17 (2015). PMID 25771421. [link to RRC of NBRP]
user_report Minamino, M., Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2. Curr. Biol. 25 (13): 1694-1706 (2015). PMID 26051894. [link to RRC of NBRP]
user_report Kikuchi, J., The novel orally active proteasome inhibitor K-7174 exerts anti-myeloma activity in vitro and in vivo by down-regulating the expression of class I histone deacetylases. J. Biol. Chem., 288 (35): 25593-25602 (2013). PMID 23878197. [link to RRC of NBRP]
user_report Matsushita, M., Meclozine Facilitates Proliferation and Differentiation of Chondrocytes by Attenuating Abnormally Activated FGFR3 Signaling in Achondroplasia. PLoS One, 8 (12): e81569 (2013). PMID 24324705. [link to RRC of NBRP]
user_report Kikuchi J., Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma. Blood., 116, 406-417 (2010). PMID 20351311. [link to RRC of NBRP]