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CSII-CMV-MCS-IRES2-Venus (#RDB04383)

Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-MCS (RDB04377) (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 30C, LB+Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB04383 CSII-CMV-MCS-IRES2-Venus DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04383).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

reference Ikemoto, M., Autologous transplantation of SM/C-2.6(+) satellite cells transduced with micro-dystrophin CS1 cDNA by lentiviral vector into mdx mice. Mo.l Ther. 15 (12): 2178-2185 (2007). PMID 17726457. [PubMed] [Article]
user_report Sato, Y., Viral Membrane-Targeting Amphipathic Helical Peptide-Based Fluorogenic Probes for the Analysis of Infectious Titers of Enveloped Viruses. Anal Chem. 2025 Jan 22. doi: 10.1021/acs.analchem.4c04852. Epub ahead of print. PMID 39840494. [PubMed] [Article] [RRC of NBRP]
user_report Suzuki, Y., Identification of APBB1 as a substrate for anaplastic lymphoma kinase. J. Biochem. 176 (5): 395-403 (2024). PMID 39115278. [PubMed] [Article] [RRC of NBRP]
user_report Nakamura, K., The periosteum provides a stromal defence against cancer invasion into the bone. Nature 634 (8033): 474-481 (2024). PMID 39169177. [PubMed] [Article] [RRC of NBRP]
user_report Mizutani, T., Neutrophil S100A9 supports M2 macrophage niche formation in granulomas. iScience 26 (3): 106081 (2023). PMID 36843852. [PubMed] [Article] [RRC of NBRP]
user_report Watanabe ,Y., Insulin-induced genes INSIG1 and INSIG2 mediate oxysterol-dependent activation of the PERK-eIF2α-ATF4 axis. J. Biol. Chem. 297 (2): 100989 (2021). PMID 34298014. [PubMed] [Article] [RRC of NBRP]
user_report Kikuchi, J., Soluble SLAMF7 promotes the growth of myeloma cells via homophilic interaction with surface SLAMF7. Leukemia 34 (1): 180-195 (2020). PMID 31358854. [PubMed] [Article] [RRC of NBRP]
user_report Ishibashi, D., Type I interferon protects neurons from prions in in vivo models. Brain 142 (4): 1035-1050 (2019). PMID 30753318. [PubMed] [Article] [RRC of NBRP]
user_report Hui, K.K., GABARAPs dysfunction by autophagy deficiency in adolescent brain impairs GABAA receptor trafficking and social behavior. Sci. Adv. 5 (4): eaau8237 (2019). PMID 30989111. [PubMed] [Article] [RRC of NBRP]
user_report Watanabe, N., A pull-down and slot blot-based screening system for inhibitor compounds of the podoplanin-CLEC-2 interaction. PLoS One 14 (9): e0222331 (2019). PMID 31553741. [PubMed] [Article] [RRC of NBRP]
user_report Kikuchi, J., Myeloma Cells are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex which Senses Lipopolysaccharide. Cancer Res. 78 (7): 1766-1778 (2018). PMID 29363546. [PubMed] [Article] [RRC of NBRP]
user_report Osada, N., Lysine-specific demethylase 1 inhibitors prevent teratoma development from human induced pluripotent stem cells. Oncotarget 9 (5): 6450-6462 (2018). PMID 29464084. [PubMed] [Article] [RRC of NBRP]
user_report Uenaka, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437. [PubMed] [Article] [RRC of NBRP]
user_report Tanaka, M., Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease. J. Clin. Invest. 127 (4): 1438-1450 (2017). PMID 28263187. [PubMed] [Article] [RRC of NBRP]
user_report Su, Z., Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes. Oncotarget 8 (63): 106296-106310 (2017). PMID 29290949. [PubMed] [Article] [RRC of NBRP]
user_report Sasamoto, Y., PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes. Sci. Rep. 6: 20807, 2016. PMID 26899008. [PubMed] [Article] [RRC of NBRP]
user_report Lu, F., Downregulation of CREB Promotes Cell Proliferation by Mediating G1/S Phase Transition in Hodgkin Lymphoma. Oncol. Res. 24 (3): 171-179 (2016). PMID 27458098. [PubMed] [Article] [RRC of NBRP]
user_report Hikita, A., Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy. Bone 76: 5-17 (2015). PMID 25771421. [PubMed] [Article] [RRC of NBRP]
user_report Minamino, M., Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2. Curr. Biol. 25 (13): 1694-1706 (2015). PMID 26051894. [PubMed] [Article] [RRC of NBRP]
user_report Kikuchi, J., The novel orally active proteasome inhibitor K-7174 exerts anti-myeloma activity in vitro and in vivo by down-regulating the expression of class I histone deacetylases. J. Biol. Chem., 288 (35): 25593-25602 (2013). PMID 23878197. [PubMed] [Article] [RRC of NBRP]
user_report Matsushita, M., Meclozine Facilitates Proliferation and Differentiation of Chondrocytes by Attenuating Abnormally Activated FGFR3 Signaling in Achondroplasia. PLoS One, 8 (12): e81569 (2013). PMID 24324705. [PubMed] [Article] [RRC of NBRP]
user_report Kikuchi J., Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma. Blood., 116, 406-417 (2010). PMID 20351311. [PubMed] [Article] [RRC of NBRP]